Human/Mouse/Rat Thioredoxin-2 Antibody

Catalog #: AF3254
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3254
AF3254-SP

Detection of Human/Mouse/Rat Thioredoxin‑2 by Western Blot.

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Human/Mouse/Rat Thioredoxin-2 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects endogenous human, mouse and rat Thioredoxin 2 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse Thioredoxin-2 Thr60-Gly166 Accession # P97493

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Immunogen affinity purified

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human/Mouse/Rat Thioredoxin‑2 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, CH-1 mouse B cell lymphoma cell line, C2C12 mouse myoblast cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat Thioredoxin-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3254) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Thioredoxin-2 at approximately 12 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Background: Thioredoxin-2

Thioredoxin-2 (Trx2), also known as mitochondrial Thioredoxin, belongs to the evolutionarily conserved Thioredoxin family. These proteins share the Thioredoxin fold containing the active site-CGPC motif. In their reduced form, the active site cysteine residues reduce protein disulfides. The resulting active site disulfide is subsequently reduced in a reaction catalyzed by a NADPH-dependent Thioredoxin reductase. Thioredoxin-2 contains an N-terminal 59 amino acid (aa) transit sequence that is cleaved upon translocation to mitochondria. The amino acid sequence of mature mouse Thioredoxin-2 is identical to mature rat Thioredoxin-2 and 98% identical to human Thioredoxin-2. Thioredoxin-2 interacts with specific components of the mitochondrial respiratory chain and helps regulate the membrane potential. Thioredoxin-2 is ubiquitously but variably expressed and high expression confers resistance to oxidant-induced apoptosis.

Entrez Gene IDs

25828 (Human); 56551 (Mouse); 79462 (Rat)

Alternate Names

mitochondrial thioredoxin; MTRX; MT-TRX; thioredoxin 2; thioredoxin, mitochondrial; Thioredoxin2; Thioredoxin-2; Trx2; TXN2

Product Datasheets

Product Datasheet

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Related Research Areas

Anti-oxidant Molecules

Citation for Human/Mouse/Rat Thioredoxin-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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