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Human/Mouse/Rat TPT1/TCTP Antibody

Catalog #: MAB4648 2 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB4648
MAB4648-SP
Detection of Human/Mouse/Rat TPT1/TCTP by Western Blot.
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Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat TPT1/TCTP Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat TPT1/TCTP in Western blots.
Source
Monoclonal Mouse IgG Clone # 488411
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human TPT1/TCTP
Met1-Cys172
Accession # P13693
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunocytochemistry
3-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse/Rat TPT1/TCTP antibody by Western Blot. View Larger

Detection of Human/Mouse/Rat TPT1/TCTP by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line, LNCaP human prostate cancer cell line, Y3-Ag rat myeloid cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Mouse/Rat TPT1/TCTP Monoclonal Antibody (Catalog # MAB4648) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human TPT1 (5 ng) was included. A specific band for TPT1/TCTP was detected at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Immunocytochemistry TPT1/TCTP antibody in HeLa Human Cell Line by Immunocytochemistry (ICC). View Larger

TPT1/TCTP in HeLa Human Cell Line. TPT1/TCTP was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line cell line using Mouse Anti-Human/Mouse/Rat TPT1/TCTP Monoclonal Antibody (Catalog # MAB4648) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TPT1/TCTP

TPT1 (translationally controlled tumor protein 1; also TCTP, HRF and fortilin) is a 23 kDa member of the TCTP family of calcium‑binding proteins. It is widely expressed (but not in kidney), and apparently serves as a transcriptional activator, calcium transporter, histamine inducer and antiapoptotic, caspase-3 inhibitor. Human TPT1 is 172 amino acids (aa) in length, with multiple potential splice variants. There is an alternate start site at Met35 that may be accompanied by a deletion of aa 99‑172. In addition, there may be either a 25 aa or 16 aa C-terminal extension after Cys172. Over aa 1‑172, human TPT1 is 96% aa identical to mouse TPT1.

Long Name
Translationally-controlled Tumor Protein 1
Entrez Gene IDs
7178 (Human); 22070 (Mouse); 116646 (Rat)
Alternate Names
FLJ27337; Fortilin; Histamine-releasing factor; HRF; p02; p23; TCTP; TCTPHRFfortilin; TPT1; translationally-controlled tumor protein; tumor protein, translationally-controlled 1

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Reviews for Human/Mouse/Rat TPT1/TCTP Antibody

Average Rating: 4.5 (Based on 2 Reviews)

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Human/Mouse/Rat TPT1/TCTP Antibody
By Anonymous on 11/29/2021
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Skin fibroblasts Species: Mouse
Human/Mouse/Rat TPT1/TCTP Antibody
By Anonymous on 12/15/2016
Application: WB Sample Tested: mammalian cell line Species: Human

Transfer a mammalian cell line with Tpt1 and use MAB4648 to perform immunoblot analysis to see if transfection occurred.