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Human/Mouse/Rat Transglutaminase 2/TGM2 Antibody

Catalog #: AF4376
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF4376
AF4376-SP
Human/Mouse/Rat Transglutaminase 2/TGM2 Antibody
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Citations (1)
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Human/Mouse/Rat Transglutaminase 2/TGM2 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human Transglutaminase 2/TGM2 in direct ELISAs and human, mouse, and rat Transglutaminase 2/TGM2 in Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) TGM3, rhTGM4, and rhTGM7 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Transglutaminase 2/TGM2 Ala2-Ala687 (Asn533Thr, Leu655Val) Accession # P21980
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated
Purity
Immunogen affinity purified

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Simple Western
5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Simple Western Detection of Human Transglutaminase 2/TGM2 antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Human Transglutaminase 2/TGM2 by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line loaded at 0.2 mg/mL. A specific band was detected for Transglutaminase 2/TGM2 at approximately 82 kDa (as indicated) using 5 µg/mL of Sheep Anti-Human/Mouse/Rat Transglutaminase 2/TGM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4376) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Background: Transglutaminase 2/TGM2

Transglutaminase 2 (TG2), encoded by the TGM2 gene, is also known as tissue transglutaminase (tTG), transgluytaminase C (TGC), and protein-glutamine-gamma -glutamyltransferase. It belongs to the family of transglutaminases that catalyze the posttranslational modification of proteins via calcium dependent cross-linking reactions (1-3). In addition to its function in protein cross-linking, TGM2 is also capable of hydrolyzing both GTP and ATP (4) and has intrinsic kinase activity (5). TGM2 has been implicated in a variety of human diseases including celiac disease, inclusion body myositis, atherosclerosis, and neurodegenerative diseases (6-7).

References
  1. Gentile, V. et al. (1991) J. Biol. Chem. 266:478.
  2. Chen, J.S.K. and K. Mehta (1999) Internat. J. Biochem. Cell Biol. 31:817.
  3. Griffin, M. et al. (2002) Biochem. J. 368:377.
  4. Lai, T.S. et al. (1998) J. Biol. Chem. 273:1776.
  5. Mishra, S. et al. (2007) J. Biol. Chem. 282:18108.
  6. Kim, S-Y. et al. (2002) Neurochem. Int. 40:85.
  7. Lesort, M. et al. (2000) Prog. Neurobiol. 61:439.
Entrez Gene IDs
7052 (Human); 21817 (Mouse); 56083 (Rat)
Alternate Names
C polypeptide; EC 2.3.2.13; G-ALPHA-h; protein-glutamine gamma-glutamyltransferase 2; protein-glutamine-gamma-glutamyltransferase; TG(C); TG2; TGase C; TGase H; TGase-2; TGase-H; TGC; TGCGNAH; TGM2; Tissue transglutaminase; transglutaminase 2 (C polypeptide, protein-glutamine-gamma-glutamyltransferase); Transglutaminase 2; Transglutaminase C; Transglutaminase H; transglutaminase-2; tTG

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Citation for Human/Mouse/Rat Transglutaminase 2/TGM2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Distinct fibroblast lineages determine dermal architecture in skin development and repair.
    Authors: Driskell R, Lichtenberger B, Hoste E, Kretzschmar K, Simons B, Charalambous M, Ferron S, Herault Y, Pavlovic G, Ferguson-Smith A, Watt F
    Nature, 2013-12-12;504(7479):277-81.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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