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Human/Mouse/Rat XIAP Antibody

Catalog #: AF8221
16 Citations Datasheet / COA / SDS
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AF8221
AF8221-SP
XIAP in Human Lymphoma.
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Citations (16)
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Human/Mouse/Rat XIAP Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse and rat XIAP in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human XIAP
Met1-Ser497 (Ser162Cys)
Accession # P98170
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Simple Western
5 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
Knockout Validated
XIAP is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in XIAP knockout HeLa cell line.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry XIAP antibody in Human Lymphoma by Immunohistochemistry (IHC-P). View Larger

XIAP in Human Lymphoma. XIAP was detected in immersion fixed paraffin-embedded sections of human lymphoma using 5 µg/mL Goat Anti-Human/Mouse/Rat XIAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8221) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry XIAP antibody in Human Breast Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

XIAP in Human Breast Cancer Tissue. XIAP was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 15 µg/mL Goat Anti-Human/Mouse/Rat XIAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8221) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Western Blot Detection of Human/Mouse/Rat XIAP antibody by Western Blot. View Larger

Detection of Human/Mouse/Rat XIAP by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, C2C12 mouse myoblast cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat XIAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8221) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for XIAP at approximately 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

Simple Western Detection of Human and Mouse XIAP antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Human and Mouse XIAP by Simple WesternTM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line and C2C12 mouse myoblast cell line, loaded at 0.2 mg/mL. A specific band was detected for XIAP at approximately 59 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse/Rat XIAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8221) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Knockout Validated Western Blot Shows Human XIAP Antibody Specificity by Using Knockout Cell Line. View Larger

Western Blot Shows Human XIAP Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and XIAP knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat XIAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8221) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for XIAP at approximately 52 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: XIAP

XIAP (X-chromosome linked inhibitor of apoptosis) is a member of the apoptosis (IAP) family of proteins that inhibit caspases. The BIR2 domain of XIAP inhibits caspase-3 and caspase-7. The ability of XIAP to inhibit caspases is prevented by SMAC/Diablo through binding to XIAP-BIR2 and -BIR3 domains.

Long Name
X-linked Inhibitor of Apoptosis
Entrez Gene IDs
331 (Human); 11798 (Mouse); 63879 (Rat)
Alternate Names
API3X-linked inhibitor of apoptosis protein; apoptosis inhibitor 3; baculoviral IAP repeat-containing 4; baculoviral IAP repeat-containing protein 4; BIRC4; BIRC4XLP2; E3 ubiquitin-protein ligase XIAP; EC 6.3.2.-; hIAP3; hIAP-3; hILP; IAP3; IAP-3; IAP-like protein; ILP; ILP1; Inhibitor of apoptosis protein 3; MIHA; XIAP; X-linked IAP; X-linked inhibitor of apoptosis

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Citations for Human/Mouse/Rat XIAP Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
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  1. cIAP1/TRAF2 interplay promotes tumor growth through the activation of STAT3
    Authors: B Dumétier, A Zadoroznyj, J Berthelet, S Causse, J Allègre, P Bourgeois, F Cattin, C Racoeur, C Paul, C Garrido, L Dubrez
    Oncogene, 2022-11-18;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Nociceptor-derived Reg3gamma prevents endotoxic death by targeting kynurenine pathway in microglia
    Authors: E Sugisawa, T Kondo, Y Kumagai, H Kato, Y Takayama, K Isohashi, E Shimosegaw, N Takemura, Y Hayashi, T Sasaki, MM Martino, M Tominaga, K Maruyama
    Cell Reports, 2022-03-08;38(10):110462.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Death agonist antibody against TRAILR2/DR5/TNFRSF10B enhances birinapant anti-tumor activity in HPV-positive head and neck squamous cell carcinomas
    Authors: Y An, J Jeon, L Sun, A Derakhshan, J Chen, S Carlson, H Cheng, C Silvin, X Yang, C Van Waes, Z Chen
    Scientific Reports, 2021-03-18;11(1):6392.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. ASTX660, a novel non-peptidomimetic antagonist of cIAP1/2 and XIAP, potently induces TNF-? dependent apoptosis in cancer cell lines and inhibits tumor growth
    Authors: GA Ward, EJ Lewis, JS Ahn, CN Johnson, JF Lyons, V Martins, JM Munck, SJ Rich, T Smyth, NT Thompson, PA Williams, NE Wilsher, NG Wallis, G Chessari
    Mol. Cancer Ther., 2018-04-25;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation, Western Blot
  5. Chronic oxycodone induces axonal degeneration in rat brain
    Authors: R Fan, LM Schrott, T Arnold, S Snelling, M Rao, D Graham, A Cornelius, NL Korneeva
    BMC Neurosci, 2018-03-23;19(1):15.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  6. Anticancer efficacy of the hypoxia-activated prodrug evofosfamide is enhanced in combination with proapoptotic receptor agonists against osteosarcoma
    Authors: V Liapis, A Zysk, M DeNichilo, I Zinonos, S Hay, V Panagopoul, A Shoubridge, C Difelice, V Ponomarev, W Ingman, GJ Atkins, DM Findlay, ACW Zannettino, A Evdokiou
    Cancer Med, 2017-08-10;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Delayed apoptosis allows islet ?-cells to implement an autophagic mechanism to promote cell survival
    Authors: HL Hayes, BS Peterson, JM Haldeman, CB Newgard, HE Hohmeier, SB Stephens
    PLoS ONE, 2017-02-17;12(2):e0172567.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Pro-Apoptotic Activity of New Honokiol/Triphenylmethane Analogues in B-Cell Lymphoid Malignancies
    Molecules, 2016-07-30;21(8):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Delivery of a survivin promoter-driven antisense survivin-expressing plasmid DNA as a cancer therapeutic: a proof-of-concept study
    Onco Targets Ther, 2016-05-03;9(0):2601-13.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. Doxorubicin overcomes resistance to drozitumab by antagonizing Inhibitor of Apoptosis Proteins (IAPs).
    Authors: Zinonos I, Labrinidis A, Liapis V, Hay S, Panagopoulos V, Denichilo M, Ponomarev V, Ingman W, Atkins G, Findlay D, Zannettino A, Evdokiou A
    Anticancer Res, 2014-12-01;34(12):7007-20.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  11. Cytotoxic activity of the amphibian ribonucleases onconase and r-amphinase on tumor cells from B cell lymphoproliferative disorders.
    Authors: Smolewski P, Witkowska M, Zwolinska M, Cebula-Obrzut B, Majchrzak A, Jeske A, Darzynkiewicz Z, Ardelt W, Ardelt B, Robak T
    Int J Oncol, 2014-04-28;45(1):419-25.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. Admixture fine-mapping in African Americans implicates XAF1 as a possible sarcoidosis risk gene.
    Authors: Levin A, Iannuzzi M, Montgomery C, Trudeau S, Datta I, Adrianto I, Chitale D, McKeigue P, Rybicki B
    PLoS ONE, 2014-03-24;9(3):e92646.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  13. Serum levels of inhibitors of apoptotic proteins (IAPs) change with IVIg therapy in pemphigus.
    Authors: Toosi, Siavash, Habib, Nancy, Torres, Geneviev, Reynolds, Sandra R, Bystryn, Jean-Cla
    J Invest Dermatol, 2011-06-30;131(11):2327-9.
    Species: Human
    Sample Types: Serum
    Applications: Western Blot
  14. Involvement of the Edar signaling in the control of hair follicle involution (catagen).
    Authors: Fessing MY, Sharova TY, Sharov AA, Atoyan R, Botchkarev VA
    Am. J. Pathol., 2006-12-01;169(6):2075-84.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  15. Acquired resistance to TRAIL-induced apoptosis in human ovarian cancer cells is conferred by increased turnover of mature caspase-3.
    Authors: Lane D, Cote M, Grondin R, Couture MC, Piche A
    Mol. Cancer Ther., 2006-03-01;5(3):509-21.
    Species: Human
    Sample Types: Whole Cells
    Applications: Western Blot
  16. Activation of NF-kappaB and upregulation of intracellular anti-apoptotic proteins via the IGF-1/Akt signaling in human multiple myeloma cells: therapeutic implications.
    Authors: Mitsiades CS, Mitsiades N, Poulaki V, Schlossman R, Akiyama M, Chauhan D, Hideshima T, Treon SP, Munshi NC, Richardson PG, Anderson KC
    Oncogene, 2002-08-22;21(37):5673-83.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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