Human/Mouse Renin R Antibody

Catalog #: AF5716 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5716
AF5716-SP

Detection of Human and Mouse Renin R by Western Blot.

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Human/Mouse Renin R Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse Renin R in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Renin R
Asn17-Glu302
Accession # O75787

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Immunogen affinity purified

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human and Mouse Renin R by Western Blot. Western blot shows lysates of human and mouse heart tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Renin R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5716) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Renin R at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Renin R

Renin R (Renin Receptor; also ATPase H⁺-transporting lysosomal accessory/interacting protein 2, M8.9 and ELDF10) is a 39‑45 kDa protein that belongs to no known family. It is expressed on the surface of macrophages, vascular smooth muscle cells, renal mesangial cells and T cells. Renin R has at least two functions. First, it binds both renin and prorenin, promoting the conversion of angiotensinogen to angiotensin I. Second, its ligation induces PAI‑1 synthesis. Mature human Renin R is a type I transmembrane protein 334 amino acids (aa) in length. It contains a 286 aa extracellular region (aa 17‑302) and a 27 aa cytoplasmic tail. It is believed to form homodimers. Potential isoforms exist. One termed M8.9 shows a deletion of aa 1‑252 and constitutes a component of the vacuolar proton ATPase. Two others show an alternate start site at Met77, and a deletion of aa 101‑132. Over aa 17‑302, the human Renin R shares 95% aa identity with the mouse Renin R.

Long Name

Renin Receptor

Entrez Gene IDs

10159 (Human); 70495 (Mouse); 302526 (Rat)

Alternate Names

APT6M8-9; ATP6AP2; ATP6IP2; ATP6M8-9; ATP6M8-9MRXE; ATPase H(+)-transporting lysosomal accessory protein 2; ATPase H(+)-transporting lysosomal-interacting protein 2; ATPase, H+ transporting, lysosomal accessory protein 2; ATPase, H+ transporting, lysosomal interacting protein 2; CAPER; ELDF10; Embryonic liver differentiation factor 10; ER-localized type I transmembrane adaptor; H+ transporting, lysosomal (vacuolar proton pump) membrane sectorassociated protein M8-9; HT028; M8-9; MGC99577; MRXE; MSTP009; N14F; Renin R; renin receptor; ReninR; vacuolar proton ATP synthase membrane sector associated protein M8-9; V-ATPase M8.9 subunit; XMRE