Human/Mouse SMOC-2 Isoform 1 Biotinylated Antibody

Catalog #: BAF5140 Datasheet / COA / SDS

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BAF5140

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Human/Mouse SMOC-2 Isoform 1 Biotinylated Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse SMOC-2 Isoform 1 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human SMOC-1 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human SMOC-2 Isoform 1
Gln22-Gly446
Accession # AAH47583

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human SMOC‑2 (Catalog # 5140-SM)

Recombinant Mouse SMOC‑2 (Catalog # 6075-SM)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SMOC-2

SMOC-2 (secreted modular calcium-binding protein 2; also SMAP2) is a 55 kDa glycoprotein member of the SPARC family of matricellular proteins. It is widely expressed, perhaps by endothelium and apparently participates in both growth factor signaling and ECM organization. Mature human SMOC-2 is 425 amino acids (aa) in length. It contains one Kazal-like domain (aa 40‑84), two thyroglobulin type-1 segments (aa 87‑153 and 213‑281) and two EF-hand sequences (aa 347‑382 and 384‑419). There are three splice variants. One shows a 13 aa substitution for aa 442‑446, another shows an 11 aa insertion after Thr170 and a third represents a combination of the above two variations. Over aa 22‑446, human SMOC-2 shares 95% aa identity with mouse SMOC-2.

Long Name

Secreted Modular Calcium-binding Protein 2

Entrez Gene IDs

64094 (Human); 64074 (Mouse); 292401 (Rat)

Alternate Names

bA270C4A.1; bA37D8.1; dJ421D16.1; MST117; MSTP117; MSTP140; SMAP2; SMAP-2; SMAP2Secreted modular calcium-binding protein 2; SMOC2; SMOC-2; smooth muscle associated protein 2; Smooth muscle-associated protein 2; SPARC related modular calcium binding 2; SPARC-related modular calcium-binding protein 2; thyroglobulin type-1 repeat containing protein