Human/Mouse Snapin Antibody

Catalog #: MAB7795 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7795
MAB7795-SP

Detection of Human and Mouse Snapin by Western Blot.

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All Snapin Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse Snapin Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human Snapin in direct ELISAs and human and mouse Snapin in Western blots.

Source

Monoclonal Mouse IgG2a Clone # 809116

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human and Mouse Snapin by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and Hepa 1-6 mouse hepatoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Snapin Monoclonal Antibody (Catalog # MAB7795) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Snapin at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: Snapin

Snapin (SNARE-associated protein) is an approximately 15 kDa, 136 amino acid vesicular phosphoprotein that contains a coiled-coil interacting domain. In neurons, it binds SNAP25 and RGS7 and regulates steps between synaptic vesicle docking and neurotransmitter release. In pancreatic beta cells, it mediates incretin-stimulated insulin secretion. In germ cells, it binds PUM2 and Nanos1 as a putative adaptor protein. Human Snapin shares 98% aa sequence identity with mouse and rat Snapin, while mouse and rat Snapin share 100% aa sequence identity with each other.

Long Name

SNAP-associated Protein

Entrez Gene IDs

23557 (Human); 20615 (Mouse)

Alternate Names

SNAP-25-binding protein; SNAP25BP; SNAPAP; SNAPAPSNARE-associated protein Snapin; SNAP-associated proteinSNARE associated protein snapin; Snapin; Synaptosomal-associated protein 25-binding protein