Human/Mouse SOD1/Cu-Zn SOD Antibody Summary
Met1-Gln154
Accession # P00441
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human/Mouse SOD1/Cu‑Zn SOD by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.2 µg/mL Goat Anti-Human/Mouse SOD1/Cu-Zn SOD Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3418) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human SOD1, SOD2, and SOD3 (1 ng/lane) were included. A specific band for SOD1/Cu-Zn SOD was detected at approximately 16-19 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
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Detection of Human SOD1/Cu‑Zn SOD by Simple WesternTM. Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for SOD1/Cu-Zn SOD at approximately 25 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse SOD1/Cu-Zn SOD Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3418) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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Detection of Human SOD1/Cu‑Zn SOD by Simple WesternTM. Simple Western lane view shows lysates of A549 human lung carcinoma cell line and SK-BR-3 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for SOD1/Cu-Zn SOD at approximately 24 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse SOD1/Cu-Zn SOD Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3418) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SOD1/Cu-Zn SOD
Superoxide Dismutases (SODs), originally identified as Indophenoloxidase (IPO), are enzymes that catalyze the converversion of naturally-occuring but harmful superoxide radicals into molecular oxygen and hydrogen peroxide. Superoxide Dismutases 1, SOD1, also known as Cu/Zn SOD, soluble SOD, and IPO-A, is a soluble, cytoplasmic 16 kDa homodimer. Each SOD1 monomer binds one Cu2+ and Zn2+ ion. Three isozymes of SOD have been identified and are functionally related but have very modest sequence homology. SOD1 shares 23% and 27% sequence identity with SOD2 and SOD3, respectively. Mutations in SOD1 have been suggested to be the cause of familial amyotrophic lateral sclerosis (ALS). The ALS-causing mutations of SOD1 are scattered throughout the protein and provide no clear functional or structural clues to the underlying disease mechanism. The oligomerization hypothesis suggests that mutant SOD1 proteins become misfolded and consequently oligomerize into high molecular weight aggregates that result in the death of motor neurons. The oxidative damage hypothesis suggests that loss of function mutations in SOD1 result in the intracellular accumulation of the superoxide radical, leading to free radical-mediated damage, the release of cytochrome c, and apoptosis.
Product Datasheets
Citations for Human/Mouse SOD1/Cu-Zn SOD Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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MSC Pretreatment for Improved Transplantation Viability Results in Improved Ventricular Function in Infarcted Hearts
Authors: MF Pittenger, S Eghtesad, PG Sanchez, X Liu, Z Wu, L Chen, BP Griffith
International Journal of Molecular Sciences, 2022-01-08;23(2):.
Species: Rat
Sample Types: Cell Lysates
Applications: Western Blot -
CETSA-based target engagement of taxanes as biomarkers for efficacy and resistance
Authors: A Langebäck, S Bacanu, H Laursen, L Mout, T Seki, S Erkens-Sch, AD Ramos, A Berggren, Y Cao, J Hartman, W van Weerde, J Bergh, P Nordlund, S Lööf
Sci Rep, 2019-12-18;9(1):19384.
Species: Human, Xenograft
Sample Types: Beads
Applications: Immunoassay -
Neuroprotective and neuritogenic activities of novel multimodal iron-chelating drugs in motor-neuron-like NSC-34 cells and transgenic mouse model of amyotrophic lateral sclerosis.
Authors: Kupershmidt L, Weinreb O, Amit T, Mandel S, Carri MT, Youdim MB
FASEB J., 2009-07-28;23(11):3766-79.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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