Human/Mouse SPRY2 Antibody Summary
Met1-Ala175
Accession # O43597
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human and Mouse SPRY2 by Western Blot. Western blot shows lysates of SH-SY5Y human neuroblastoma cell line, PC-3 human prostate cancer cell line, and C2C12 mouse myoblast cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse SPRY2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6157) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for SPRY2 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Human and Mouse SPRY2 by Simple WesternTM. Simple Western lane view shows lysates of C2C12 mouse myoblast cell line and SH-SY5Y human neuroblastoma cell line, loaded at 0.2 mg/mL. A specific band was detected for SPRY2 at approximately 45 kDa (as indicated) using 50 µg/mL of Goat Anti-Human/Mouse SPRY2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6157) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SPRY2
SPRY2 (sprouty homolog 2) is a 35 kDa member of the sprouty family of proteins. It is widely expressed in embryonic and adult tissues, including endothelium. SPRY2 is considered a negative regulator of Ras/ERK signaling. For example, it appears to form a trimeric complex with PKCδ and PKD1, blocking ERK phosphorylation. It also interacts with a number of other molecules, including CBL and SIAH2, which likely limit its availability. Human SPRY2 is 315 amino acids (aa) in length and contains one CBL-TKB binding site (aa 53‑59) that is phosphorylated at Tyr55, a Ser-rich region (aa 125‑131), a Cys-rich domain (aa 178‑301) and a PxxPxR motif that mediates ERK inhibitory activity. Over aa 1‑175, human SPRY2 shares 94% aa identity with mouse SPRY2.
Product Datasheets
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