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Human/Mouse SSEA-4 APC-conjugated Antibody

Catalog #: FAB1435A
18 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB1435A-100
FAB1435A-025
Detection of SSEA‑4 in NTera‑2 Human Cell Line by Flow Cytometry.
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Product Details
Citations (18)
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Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse SSEA-4 APC-conjugated Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Recognizes a carbohydrate epitope of SSEA-4 (1, 2).
Source
Monoclonal Mouse IgG3 Clone # MC-813-70
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
2120Ep human embryonal carcinoma cell line
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of SSEA-4 antibody in NTera-2 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of SSEA‑4 in NTera‑2 Human Cell Line by Flow Cytometry. NTera-2 human testicular embryonic carcinoma cell line was stained with Mouse Anti-Human/Mouse SSEA-4 APC-conjugated Monoclonal Antibody (Catalog # FAB1435A, filled histogram) or isotype control antibody (Catalog # IC007A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: SSEA-4

SSEA-4 is expressed on the surface of human embryonal carcinoma (EC) cells (the pluripotent stem cells of teratocarcinomas), human embryonic germ cells (EG), and human embryonic stem cells (ES). Expression of SSEA-4 is down-regulated following differentiation of human EC cells. In contrast, the differentiation of murine EC and ES cells may be accompanied by an increase in SSEA-4 expression (1-4).

References
  1. Shevinsky, L.H. et al. (1982) Cell 30:697.
  2. Kannagi, R. et al. (1983) EMBO J. 2:2355.
  3. Thomson, J.A. and J.S. Odorico (2000) Trends Biotechnol. 18:53.
  4. Draper, J.S. et al. (2002) J. Anat. 200:249.
Long Name
Stage-specific Embryonic Antigen-4
Alternate Names
SSEA4; SSEA-4; SSEA-4;stage-specific embryonic antigen 4

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Citations for Human/Mouse SSEA-4 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

18 Citations: Showing 1 - 10
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  1. Revealing cell populations catching the early stages of human embryo development in naive pluripotent stem cell cultures
    Authors: M Moya-Jódar, A Ullate-Ago, P Barlabé, JR Rodríguez-, G Abizanda, C Barreda, X Carvajal-V, A Vilas-Zorn, JP Romero, L Garate, X Agirre, G Coppiello, F Prósper, XL Aranguren
    Stem Cell Reports, 2022-12-22;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Protection of nascent DNA at stalled replication forks is mediated by phosphorylation of RIF1 intrinsically disordered region
    Authors: S Balasubram, M Andreani, JG Andrade, T Saha, D Sundaravin, J Garzón, W Zhang, O Popp, SI Hiraga, A Rahjouei, DB Rosen, P Mertins, BT Chait, AD Donaldson, M Di Virgili
    Elife, 2022-04-13;11(0):.
    Species: Human
    Sample Types: Whole Cell
    Applications: IF
  3. Multi-omic rejuvenation of human cells by maturation phase transient reprogramming
    Authors: D Gill, A Parry, F Santos, H Okkenhaug, CD Todd, I Hernando-H, TM Stubbs, I Milagre, W Reik
    Elife, 2022-04-08;11(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC/IF
  4. Generation of three human induced pluripotent stem cell sublines (UCLAi004-A, UCLAi004-B, and UCLAi004-C) for reproductive science research
    Authors: EC Pandolfi, TJ Hunt, S Goldsmith, K Hurlbut, SJ Silber, AT Clark
    Stem Cell Research, 2021-06-24;54(0):102446.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  5. Generation of three human induced pluripotent stem cell sublines (UCLAi005-A, UCLAi005-B and UCLAi005-C) for reproductive science research
    Authors: EC Pandolfi, TJ Hunt, S Goldsmith, K Hurlbut, SJ Silber, AT Clark
    Stem Cell Research, 2021-06-08;54(0):102409.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  6. Modeling Progressive Fibrosis with Pluripotent Stem Cells Identifies an Anti-fibrotic Small Molecule
    Authors: P Vijayaraj, A Minasyan, A Durra, S Karumbayar, M Mehrabi, CJ Aros, SD Ahadome, DW Shia, K Chung, JM Sandlin, KF Darmawan, KV Bhatt, CC Manze, MK Paul, DC Wilkinson, W Yan, AT Clark, TM Rickabaugh, WD Wallace, TG Graeber, R Damoiseaux, BN Gomperts
    Cell Rep, 2019-12-10;29(11):3488-3505.e9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Generation of three human induced pluripotent stem cell sublines (MZT04D, MZT04J, MZT04C) for reproductive science research
    Authors: EC Pandolfi, EJ Rojas, E Sosa, JJ Gell, TJ Hunt, S Goldsmith, Y Fan, SJ Silber, AT Clark
    Stem Cell Res, 2019-09-16;40(0):101576.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Generation and characterization of two human induced pluripotent stem cell (hiPSC) lines homozygous for the Apolipoprotein e4 (APOE4) risk variant-Alzheimer's disease (ASUi005-A) and healthy non-demented control (ASUi006-A)
    Authors: N Brookhouse, P Zhang, R Caselli, JJ Kim, DA Brafman
    Stem Cell Res, 2018-09-21;32(0):145-149.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Generation of hiPSTZ16 (ISMMSi003-A) cell line from normal human foreskin fibroblasts
    Authors: M Dejosez, TP Zwaka
    Stem Cell Res, 2017-12-02;26(0):44-46.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Establishment of an induced pluripotent stem (iPS) cell line from dermal fibroblasts of an asymptomatic patient with dominant PRPF31 mutation
    Authors: A Terray, V Fort, A Slembrouck, C Nanteau, JA Sahel, S Reichman, I Audo, O Goureau
    Stem Cell Res, 2017-10-07;25(0):26-29.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Generation of an induced pluripotent stem cell (iPSC) line from a patient with autosomal dominant retinitis pigmentosa due to a mutation in the NR2E3 gene
    Authors: A Terray, A Slembrouck, C Nanteau, C Chondroyer, C Zeitz, JA Sahel, I Audo, S Reichman, O Goureau
    Stem Cell Res, 2017-08-05;24(0):1-4.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. Generation and characterization of human induced pluripotent stem cell (hiPSC) lines from an Alzheimer's disease (ASUi001-A) and non-demented control (ASUi002-A) patient homozygous for the Apolipoprotein e4 (APOE4) risk variant
    Authors: N Brookhouse, P Zhang, R Caselli, JJ Kim, DA Brafman
    Stem Cell Res, 2017-06-08;24(0):160-163.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Prospectively Isolated Human Bone Marrow Cell-Derived MSCs Support Primitive Human CD34-Negative Hematopoietic Stem Cells.
    Authors: Matsuoka Y, Nakatsuka R, Sumide K, Kawamura H, Takahashi M, Fujioka T, Uemura Y, Asano H, Sasaki Y, Inoue M, Ogawa H, Takahashi T, Hino M, Sonoda Y
    Stem Cells, 2015-05-01;33(5):1554-65.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. JMJD5 regulates cell cycle and pluripotency in human embryonic stem cells.
    Authors: Zhu H, Hu S, Baker J
    Stem Cells, 2014-08-01;32(8):2098-110.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  15. Human embryonic stem cell-derived mesenchymal stroma cells (hES-MSCs) engraft in vivo and support hematopoiesis without suppressing immune function: implications for off-the shelf ES-MSC therapies.
    Authors: Li O, Tormin A, Sundberg B, Hyllner J, Le Blanc K, Scheding S
    PLoS ONE, 2013-01-29;8(1):e55319.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. Evaluation of ABCG2 expression in human embryonic stem cells: crossing the same river twice?
    Authors: Sarkadi B, Orban TI, Szakacs G, Varady G, Schamberger A, Erdei Z, Szebenyi K, Homolya L, Apati A
    Stem Cells, 2010-01-01;28(1):174-6.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  17. High concentration of synthetic serum, stepwise equilibration and slow cooling as an efficient technique for large-scale cryopreservation of human embryonic stem cells.
    Authors: Lee JY, Lee JE, Kim DK, Yoon TK, Chung HM, Lee DR
    Fertil. Steril., 2008-11-19;93(3):976-85.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  18. Expression and functional analysis of G1 to S regulatory components reveals an important role for CDK2 in cell cycle regulation in human embryonic stem cells.
    Authors: Neganova I, Zhang X, Atkinson S, Lako M
    Oncogene, 2008-09-22;28(1):20-30.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Human/Mouse SSEA‑4 APC‑conjugated Antibody
By Anonymous on 10/26/2015
Application: Flow Sample Tested: Human embryonic stem cells (H9 from Wicell) Species: Human

hPSC were grown for 20 passages as monolayers on matrigel.
Specificity: Specific
Sensitivity: Reasonably sensitive
Buffer: PBS+.1% FBS
Dilution: 1:20

Flow Cytometry Human/Mouse SSEA‑4 APC‑conjugated Antibody FAB1435A