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Human/Mouse STAT5a/b Pan Specific Antibody

Catalog #: AF2168
15 Citations 1 Review Datasheet / COA / SDS
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AF2168
AF2168-SP
Detection of Human/Mouse STAT5a/b by Western Blot.
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Citations (15)
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse STAT5a/b Pan Specific Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects endogenous human and mouse STAT5A and STAT5B in Western blots.
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
Human STAT5a and STAT5b synthetic peptide
aa 219-390
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
20 µg/mL
See below
Chromatin Immunoprecipitation (ChIP)
5 µg/5 x 106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse STAT5a/b antibody by Western Blot. View Larger

Detection of Human/Mouse STAT5a/b by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and M1 mouse myeloid leukemia cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human/Mouse STAT5a/b Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2168) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for STAT5a at approximately 94 kDa and STAT5b at approximately 92 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Chromatin Immunoprecipitation (ChIP) Detection of STAT5a/b-regulated Genes antibody by Chromatin Immunoprecipitation. View Larger

Detection of STAT5a/b-regulated Genes by Chromatin Immunoprecipitation. BaF3 mouse pro-B cell line treated with 10 ng/mL Recombinant Mouse IL-3 (Catalog # 403-ML) for 1 hour was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. STAT5a/b/DNA complexes were immunoprecipitated using 5 µg Rabbit Anti-Human/Mouse STAT5a/b Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2168) or control antibody (Catalog # AB-105-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Rabbit IgG Secondary Antibody (Catalog # BAF008). Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. Thebcl-xpromoter was detected by standard PCR.

Simple Western Detection of Human STAT5a/b antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Human STAT5a/b by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and K562 human chronic myelogenous leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for STAT5a/b at approximately 91 kDa (as indicated) using 20 µg/mL of Rabbit Anti-Human/Mouse STAT5a/b Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2168). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: STAT5a/b

Signal transduction and activator of transcription 5 (STAT5) is a member of the Jak/STAT signal transduction pathway and is activated by a variety of cytokines (IL22, IL6, IFN-α). STAT5 has two isoforms (A and B) that share 93% amino acid identity and bind the DNA consensus site TTCN3GAA. STAT5 mediates cytokine signaling by acting as a signal transducer in the cytoplasm and, upon phosphorylation, translocates to the nucleus and activates transcription of specific genes. STAT5 is involved in a wide array of biological processes ranging from regulating apoptosis to adult mammary gland proliferation, differentiation and survival.

Long Name
Signal Transducer and Activator of Transcription 5b
Alternate Names
MGF; STAT5; STAT5A signal transducer and activator of transcription 5A ; STAT5a/b

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Citations for Human/Mouse STAT5a/b Pan Specific Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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  1. Aiolos represses CD4+ T cell cytotoxic programming via reciprocal regulation of TFH transcription factors and IL-2 sensitivity
    Authors: KA Read, DM Jones, S Pokhrel, EDS Hales, A Varkey, JA Tuazon, CD Eisele, O Abdouni, A Saadey, MR Leonard, RT Warren, MD Powell, JM Boss, EA Hemann, JS Yount, G Xin, HE Ghoneim, CJ Lio, AG Freud, PL Collins, KJ Oestreich
    Nature Communications, 2023-03-24;14(1):1652.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: ChIP
  2. Deconvoluting global cytokine signaling networks in natural killer cells
    Authors: GM Wiedemann, EK Santosa, S Grassmann, S Sheppard, JB Le Luduec, NM Adams, C Dang, KC Hsu, JC Sun, CM Lau
    Nature Immunology, 2021-04-15;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: ChIP
  3. Tackling resistance in chronic myeloid leukemia: Novel cell death modulators with improved efficacy
    Authors: AM Schoepf, S Salcher, P Obexer, R Gust
    European Journal of Medicinal Chemistry, 2021-02-18;216(0):113285.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Divergent Role for STAT5 in the Adaptive Responses of Natural Killer Cells
    Authors: GM Wiedemann, S Grassmann, CM Lau, M Rapp, AV Villarino, C Friedrich, G Gasteiger, JJ O'Shea, JC Sun
    Cell Reports, 2020-12-15;33(11):108498.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: ChIP, Flow Cytometry
  5. Ikaros antagonizes DNA binding by STAT5 in pre-B cells
    Authors: B Heizmann, S Le Gras, C Simand, P Marchal, S Chan, P Kastner
    PLoS ONE, 2020-11-12;15(11):e0242211.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: ChIP
  6. Benzodiazepines Drive Alteration of Chromatin at the Integrated HIV-1 LTR
    Authors: W Elbezanti, A Lin, A Schirling, A Jackson, M Marshall, RV Duyne, F Maldarelli, L Sardo, Z Klase
    Viruses, 2020-02-09;12(2):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  7. Elevated Prolactin during Pregnancy Drives a Phenotypic Switch in Mouse Hypothalamic Dopaminergic Neurons
    Authors: SH Yip, N Romanò, P Gustafson, DJ Hodson, EJ Williams, IC Kokay, AO Martin, P Mollard, DR Grattan, SJ Bunn
    Cell Rep, 2019-02-12;26(7):1787-1799.e5.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  8. Phosphorylated STAT5 directly facilitates parvovirus B19 DNA replication in human erythroid progenitors through interaction with the MCM complex
    Authors: SS Ganaie, W Zou, P Xu, X Deng, S Kleiboeker, J Qiu
    PLoS Pathog., 2017-05-01;13(5):e1006370.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. Identification of Novel STAT6-Regulated Proteins in Mouse B Cells by Comparative Transcriptome and Proteome Analysis
    Authors: L Mokada-Gop, A Boeser, CH Lehmann, F Drepper, D Dudziak, B Warscheid, D Voehringer
    J. Immunol, 2017-03-27;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. STAT5 Orchestrates Local Epigenetic Changes for Chromatin Accessibility and Rearrangements by Direct Binding to the TCRgamma Locus.
    Authors: Wagatsuma K, Tani-ichi S, Liang B, Shitara S, Ishihara K, Abe M, Miyachi H, Kitano S, Hara T, Nanno M, Ishikawa H, Sakimura K, Nakao M, Kimura H, Ikuta K
    J Immunol, 2015-07-20;195(4):1804-14.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ChIP
  11. The TNF-family ligand TL1A and its receptor DR3 promote T cell-mediated allergic immunopathology by enhancing differentiation and pathogenicity of IL-9-producing T cells.
    Authors: Richard A, Tan C, Hawley E, Gomez-Rodriguez J, Goswami R, Yang X, Cruz A, Penumetcha P, Hayes E, Pelletier M, Gabay O, Walsh M, Ferdinand J, Keane-Myers A, Choi Y, O'Shea J, Al-Shamkhani A, Kaplan M, Gery I, Siegel R, Meylan F
    J Immunol, 2015-03-18;194(8):3567-82.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: ChIP
  12. Reversal of acquired drug resistance in FLT3-mutated acute myeloid leukemia cells via distinct drug combination strategies.
    Authors: Zhang W, Gao C, Konopleva M, Chen Y, Jacamo R, Borthakur G, Cortes J, Ravandi F, Ramachandran A, Andreeff M
    Clin Cancer Res, 2014-03-11;20(9):2363-74.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  13. Opposing actions of IL-2 and IL-21 on Th9 differentiation correlate with their differential regulation of BCL6 expression.
    Authors: Liao W, Spolski R, Li P, Du N, West E, Ren M, Mitra S, Leonard W
    Proc Natl Acad Sci U S A, 2014-02-18;111(9):3508-13.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ChIP
  14. Cutaneous T cell lymphoma expresses immunosuppressive CD80 (B7-1) cell surface protein in a STAT5-dependent manner.
    Authors: Zhang, Qian, Wang, Hong Yi, Wei, Fang, Liu, Xiaobin, Paterson, Jennifer, Roy, Darshan, Mihova, Daniela, Woetmann, Anders, Ptasznik, Andrzej, Odum, Niels, Schuster, Stephen, Marafioti, Teresa, Riley, James L, Wasik, Mariusz
    J Immunol, 2014-02-12;192(6):2913-9.
    Species: Human
    Sample Types: Cell Extracts, Cell Lysates
    Applications: ChIP, Functional Assay
  15. Growth factor independent-1 induced by IL-4 regulates Th2 cell proliferation.
    Authors: Zhu J, Guo L, Min B, Watson CJ, Hu-Li J, Young HA, Tsichlis PN, Paul WE
    Immunity, 2002-05-01;16(5):733-44.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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Human/Mouse STAT5a/b Pan Specific Antibody
By Anonymous on 06/04/2019
Application: ChIP Sample Tested: Alzheimer's disease brain Species: Human