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Human/Mouse TREM2 Antibody

Catalog #: MAB17291
22 Citations 2 Reviews Datasheet / COA / SDS
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MAB17291-SP
MAB17291-500
MAB17291-100
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Detection of TREM2 in RAW 264.7 Mouse Cell Line by Flow Cytometry.
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Citations (22)
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Reviews (2)
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Human/Mouse TREM2 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse TREM2 in direct ELISAs. Stains TREM2 transfectants but not TREM1 transfectants in flow cytometry. In sandwich ELISAs, detects mouse TREM2 when paired with a mouse TREM2 detection antibody.
Source
Monoclonal Rat IgG2B Clone # 237920
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse TREM2
extracellular domain
Accession # Q99NH8
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
ELISA

This antibody functions as a mouse TREM2 ELISA capture antibody when paired with Sheep Anti-Mouse TREM2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1729).

This product is intended for assay development on various assay platforms requiring antibody pairs.

 
Flow Cytometry
2.5 µg/106 cells
See below
Immunohistochemistry
0.5-15 µg/mL
Immersion fixed paraffin-embedded sections of Mouse Spleen
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
5-25 µg/mL
immersion fixed RAW 264.7 mouse monocyte/macrophage cell line
Knockout Validated
TREM2 was detected in immersion fixed RAW 264.7 mouse cell line but is not detected TREM2 knockout (KO) RAW 264.7 mouse cell line.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of TREM2 antibody in RAW 264.7 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of TREM2 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Human/Mouse TREM2 Monoclonal Antibody (Catalog # MAB17291, filled histogram) or isotype control antibody (MAB0061, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')2Secondary Antibody (F0105B).

Flow Cytometry Detection of TREM2 antibody in THP-1 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of TREM2 in THP‑1 Human Cell Line by Flow Cytometry. THP-1 human acute monocytic leukemia cell line was stained with Rat Anti-Human/Mouse TREM2 Monoclonal Antibody (Catalog # MAB17291, filled histogram) or isotype control antibody (MAB0061, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (F0113).

Flow Cytometry Detection of TREM2 antibody in Human Mature Dendritic Cells antibody by Flow Cytometry. View Larger

Detection of TREM2 in Human Mature Dendritic Cells by Flow Cytometry. Human mature dendritic cells were stained with Rat Anti-Human/Mouse TREM2 Monoclonal Antibody (Catalog # MAB17291, filled histogram) or isotype control antibody (MAB0061, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (F0113).

Flow Cytometry Detection of TREM2 antibody in HEK293 Human Cell Line Transfected with eGFP and Either Human TREM2 or Human TREM1 antibody by Flow Cytometry. View Larger

Detection of TREM2 in HEK293 Human Cell Line Transfected with eGFP and Either Human TREM2 or Human TREM1 by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with eGFP and either (A) human TREM2 or (B) human TREM1 was stained with Rat Anti-Human/Mouse TREM2 Monoclonal Antibody (Catalog # MAB17291) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (F0113). Quadrant markers were set based on control antibody staining (MAB0061).

Immunocytochemistry TREM2 Specificity is Shown by Immunocytochemistry antibody in Knockout Cell Line by Immunocytochemistry (ICC). View Larger

TREM2 Specificity is Shown by Immunocytochemistry in Knockout Cell Line. Triggering Receptor Expressed on Myeloid Cells 2 (TREM2) was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line but is not detected in TREM2 knockout (KO) RAW 264.7 Mouse Cell Line cell line using Rat Anti-Human/Mouse TREM2 Monoclonal Antibody (Catalog # MAB17291) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.Staining was performed using our Fluorescent ICC Staining of Cells on Coverslips.

ELISA Mouse TREM2 Antibody in ELISA Standard Curve. View Larger

Mouse TREM2 ELISA Standard Curve. Recombinant Mouse TREM2 protein was serially diluted 2-fold and captured by Rat Anti-Human/Mouse TREM2 Monoclonal Antibody (Catalog # MAB17291) coated on a Clear Polystyrene Microplate (DY990). Sheep Anti-Mouse TREM2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (BAF1729) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).

Immunohistochemistry View Larger

Detection of TREM2 in Mouse Spleen. TREM2 was detected in immersion fixed paraffin-embedded sections of Mouse Spleen using Rat Anti-Human/Mouse TREM2 Monoclonal Antibody (Catalog # MAB17291) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC005). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to Secretion & cell surface. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TREM2

TREM2 (Triggering Receptor Expressed on Myeloid cells-2) is a 35 kDa molecular weight type I transmembrane member of the TREM family and Ig superfamily. Mature human TREM2 consists of a 156 amino acid (aa) extracellular domain (ECD) with one V-type Ig-like domain, a 21 aa transmembrane (TM) domain, and a 35 aa cytoplasmic tail. Within the ECD, human TREM2 shares 73% and 74% aa sequence identity with mouse and rat TREM2, respectively. Two closely related transcripts were reported in mouse and designated TREM2a and TREM2b. Soluble forms of the TREM2 ECD are generated by alternative splicing or proteolytic cleavage, and the cytoplasmic domain can be liberated by gamma-Secretase mediated intramembrane cleavage. It is a pattern recognition receptor that binds anionic ligands. A positively charged lysine within the transmembrane segment allows association with the signal adapter protein, DAP12 to deliver an activating signal that plays a role in both innate and adaptive immune responses, including inhibition of macrophage activation. TREM2 is expressed on macrophages, immature myeloid dendritic cells, osteoclasts, microglia, and adipocytes. It promotes the differentiation and function of osteoclasts, the production of inflammatory cytokines by adipocytes, insulin resistance, and the phagocytic clearance of bacteria. In the CNS, TREM2 binds to ApoE, ApoA1, and ApoB and mediates the clearance of apoptotic neurons, amyloid plaques, and cell debris following demyelination. TREM2 also interacts with and modifies signaling through Plexin A1 on dendritic cells and osteoclasts. Mutations in TREM2 or DAP12 are associated with the development of Alzheimer's disease and Nasu-Hakola disease (NHD/PLOSL) which is characterized by presenile dementia and bone cysts. Soluble TREM2 is elevated in cerebrospinal fluid of patients with active multiple sclerosis (MS), and TREM2 blockade exacerbates disease symptoms in the experimental EAE model of MS.

Long Name
Triggering Receptor Expressed on Myeloid Cells 2
Entrez Gene IDs
54209 (Human); 102133279 (Cynomolgus Monkey)
Alternate Names
PLOSL2; TREM2; TREM-2; Trem2a; Trem2b; Trem2c; TREM-2triggering receptor expressed on myeloid cells 2a; Triggering receptor expressed on monocytes 2; triggering receptor expressed on myeloid cells 2

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Citations for Human/Mouse TREM2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

22 Citations: Showing 1 - 10
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  1. sTREM2 Differentially Affects Cytokine Expression in Myeloid-Derived Cell Models via MAPK-JNK Signaling Pathway
    Authors: Arsenault, R;Marshall, S;Salois, P;Li, Q;Zhang, W;
    Biology
    Species: Human hepegivirus
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. TREM2 expression on the microglia resolved lead exposure-induced neuroinflammation by promoting anti-inflammatory activities
    Authors: Su, P;Zhang, J;Wu, J;Chen, H;Luo, W;Hu, M;
    Ecotoxicology and environmental safety
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Skin basal cell carcinomas assemble a pro-tumorigenic spatially organized and self-propagating Trem2+ myeloid niche
    Authors: Haensel, D;Daniel, B;Gaddam, S;Pan, C;Fabo, T;Bjelajac, J;Jussila, AR;Gonzalez, F;Li, NY;Chen, Y;Hou, J;Patel, T;Aasi, S;Satpathy, AT;Oro, AE;
    Nature communications
    Species:  Human
    Sample Types: Organoids
    Applications: Neutralization
  4. Negative regulation of TREM2-mediated C9orf72 poly-GA clearance by the NLRP3 inflammasome
    Authors: X Shu, C Wei, WY Tu, K Zhong, S Qi, A Wang, L Bai, SX Zhang, B Luo, ZZ Xu, K Zhang, C Shen
    Cell Reports, 2023-02-16;42(2):112133.
    Species: Mouse
    Sample Types: Cerebrospinal Fluid
    Applications: ELISA Capture
  5. A TREM2-activating antibody with a blood-brain barrier transport vehicle enhances microglial metabolism in Alzheimer's disease models
    Authors: B van Lenger, L Zhan, D Xia, D Chan, D Joy, JI Park, D Tatarakis, M Calvert, S Hummel, S Lianoglou, ME Pizzo, R Prorok, E Thomsen, LM Bartos, P Beumers, A Capell, SS Davis, L de Weerd, JC Dugas, J Duque, T Earr, K Gadkar, T Giese, A Gill, J Gnörich, C Ha, M Kannuswamy, DJ Kim, ST Kunte, LH Kunze, D Lac, K Lechtenber, AW Leung, CC Liang, I Lopez, P McQuade, A Modi, VO Torres, HN Nguyen, I Pesämaa, N Propson, M Reich, Y Robles-Col, K Schlepckow, L Slemann, H Solanoy, JH Suh, RG Thorne, C Vieira, K Wind-Mark, K Xiong, YJY Zuchero, D Diaz, MS Dennis, F Huang, K Scearce-Le, RJ Watts, C Haass, JW Lewcock, G Di Paolo, M Brendel, PE Sanchez, KM Monroe
    Nature Neuroscience, 2023-01-12;0(0):.
    Species: Human
    Sample Types: Transfected Whole Cells
    Applications: Flow Cytometry
  6. Naturally-aged microglia exhibit phagocytic dysfunction accompanied by gene expression changes reflective of underlying neurologic disease
    Authors: AL Thomas, MA Lehn, EM Janssen, DA Hildeman, CA Chougnet
    Scientific Reports, 2022-11-14;12(1):19471.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Human iPSC co-culture model to investigate the interaction between microglia and motor neurons
    Authors: BF Vahsen, E Gray, A Candalija, KML Cramb, J Scaber, R Dafinca, A Katsikoudi, Y Xu, L Farrimond, R Wade-Marti, WS James, MR Turner, SA Cowley, K Talbot
    Scientific Reports, 2022-07-23;12(1):12606.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  8. Microglial? signalling pathway deficits associated with the patient derived R47H TREM2 variants linked to AD indicate inability to activate inflammasome
    Authors: K Cosker, A Mallach, J Limaye, TM Piers, J Staddon, SJ Neame, J Hardy, JM Pocock
    Scientific Reports, 2021-06-25;11(1):13316.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  9. Generation of cryopreserved macrophages from normal and genetically engineered human pluripotent stem cells for disease modelling
    Authors: C Munn, S Burton, S Dickerson, K Bakshy, A Strouse, D Rajesh
    PLoS ONE, 2021-04-22;16(4):e0250107.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  10. Monocytic Infiltrates Contribute to Autistic-like Behaviors in a Two-Hit Model of Neurodevelopmental Defects
    Authors: HR Chen, CW Chen, N Mandhani, JC Short-Mill, MR Smucker, YY Sun, CY Kuan
    J Neurosci, 2020-10-30;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Resveratrol mediates mechanical allodynia through modulating inflammatory response via the TREM2-autophagy axis in SNI rat model
    Authors: Y Wang, Y Shi, Y Huang, W Liu, G Cai, S Huang, Y Zeng, S Ren, H Zhan, W Wu
    J Neuroinflammation, 2020-10-21;17(1):311.
    Species: Rat
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Western Blot
  12. Noncanonical function of an autophagy protein prevents spontaneous Alzheimer's disease
    Authors: BL Heckmann, BJW Teubner, E Boada-Rome, B Tummers, C Guy, P Fitzgerald, U Mayer, S Carding, SS Zakharenko, T Wileman, DR Green
    Sci Adv, 2020-08-14;6(33):eabb9036.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Receptor Recycling, Western Blot
  13. Alzheimer's-associated PLCgamma2 is a signaling node required for both TREM2 function and the inflammatory response in human microglia
    Authors: BJ Andreone, L Przybyla, C Llapashtic, A Rana, SS Davis, B van Lenger, K Lin, J Shi, Y Mei, G Astarita, G Di Paolo, T Sandmann, KM Monroe, JW Lewcock
    Nat. Neurosci., 2020-06-08;0(0):.
    Species: Human, Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. Opposite microglial activation stages upon loss of PGRN or TREM2 result in reduced cerebral glucose metabolism
    Authors: JK Götzl, M Brendel, G Werner, S Parhizkar, L Sebastian, G Kleinberge, AV Colombo, M Deussing, M Wagner, J Winkelmann, J Diehl-Schm, J Levin, K Fellerer, A Reifschnei, S Bultmann, P Bartenstei, A Rominger, S Tahirovic, ST Smith, C Madore, O Butovsky, A Capell, C Haass
    EMBO Mol Med, 2019-06-01;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA Detection
  15. Soluble TREM2 ameliorates pathological phenotypes by modulating microglial functions in an Alzheimer's disease model
    Authors: L Zhong, Y Xu, R Zhuo, T Wang, K Wang, R Huang, D Wang, Y Gao, Y Zhu, X Sheng, K Chen, N Wang, L Zhu, D Can, Y Marten, M Shinohara, CC Liu, D Du, H Sun, L Wen, H Xu, G Bu, XF Chen
    Nat Commun, 2019-03-25;10(1):1365.
    Species: Mouse, Transgenic Mouse
    Sample Types: Cell Culture Supernates, Tissue Homogenates
    Applications: ELISA Development (Capture)
  16. Expansion and activation of distinct central memory T lymphocyte subsets in complex regional pain syndrome
    Authors: MA Russo, NT Fiore, C van Vreden, D Bailey, DM Santarelli, HM McGuire, B Fazekas de, PJ Austin
    J Neuroinflammation, 2019-03-18;16(1):63.
    Species: Human
    Sample Types: Whole Cells
    Applications: CyTof
  17. Human Induced Pluripotent Stem Cell-Derived Microglia-Like Cells Harboring TREM2 Missense Mutations Show Specific Deficits in Phagocytosis
    Authors: P Garcia-Rei, A Phillips, TM Piers, C Villegas-L, M Butler, A Mallach, C Rodrigues, CE Arber, A Heslegrave, H Zetterberg, H Neumann, S Neame, H Houlden, J Hardy, JM Pocock
    Cell Rep, 2018-08-28;24(9):2300-2311.
    Species: Human
    Sample Types: Cell Culture Supernates
  18. A split-luciferase complementation, real-time reporting assay enables monitoring of the disease-associated transmembrane protein TREM2 in live cells
    Authors: MM Varnum, KA Clayton, A Yoshii-Kit, G Yonemoto, L Koro, S Ikezu, T Ikezu
    J. Biol. Chem., 2017-05-10;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  19. Interleukin-2 improves amyloid pathology, synaptic failure and memory in Alzheimer's disease mice
    Authors: Sandro Alves
    Brain, 2017-03-01;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  20. Nuclear receptors license phagocytosis by trem2+ myeloid cells in mouse models of Alzheimer's disease.
    Authors: Savage J, Jay T, Goduni E, Quigley C, Mariani M, Malm T, Ransohoff R, Lamb B, Landreth G
    J Neurosci, 2015-04-22;35(16):6532-43.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  21. TREM-2 promotes macrophage survival and lung disease after respiratory viral infection.
    Authors: Wu K, Byers D, Jin X, Agapov E, Alexander-Brett J, Patel A, Cella M, Gilfilan S, Colonna M, Kober D, Brett T, Holtzman M
    J Exp Med, 2015-04-20;212(5):681-97.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: Flow Cytometry, IHC-P
  22. Triggering receptor expressed on myeloid cells 2 (TREM2) deficiency attenuates phagocytic activities of microglia and exacerbates ischemic damage in experimental stroke.
    Authors: Kawabori M, Kacimi R, Kauppinen T, Calosing C, Kim J, Hsieh C, Nakamura M, Yenari M
    J Neurosci, 2015-02-25;35(8):3384-96.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC

FAQs

  1. What type of light chain does the Human/Mouse TREM-2 Antibody, Cat# MAB17291, clone # 237920 have?

    • This antibody has a kappa light chain.

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Reviews for Human/Mouse TREM2 Antibody

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Human/Mouse TREM-2 Antibody
By Leslie Priddy on 04/13/2018
Application: IHC Sample Tested: Tumor cell lyastes Species: Mouse
Human/Mouse TREM-2 Antibody
By Ju Shi on 12/01/2017
Application: Flow Sample Tested: 293T human embryonic kidney cell line Species: Human