Human/Mouse WASP Antibody Summary
Lys230-Gln310
Accession # P42768
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
![Detection of Human, Mouse and Rat WASP antibody by Western Blot. Detection of Human, Mouse and Rat WASP antibody by Western Blot.](https://resources.rndsystems.com/images/datasheets/antibody/WASP_AF3070_Western_Blot_5242.jpg)
Detection of Human, Mouse and Rat WASP by Western Blot. Western blot shows lysates of NRK rat normal kidney cell, K562 human chronic myelogenous leukemia cell line, and Balb/3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse WASP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3070) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for WASP at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
![WASP antibody in Jurkat Human Cell Line by Immunocytochemistry (ICC). WASP antibody in Jurkat Human Cell Line by Immunocytochemistry (ICC).](https://resources.rndsystems.com/images/datasheets/antibody/WASP_AF3070_Immunocytochemistry__Immunofluorescence_19228.jpg)
WASP in Jurkat Human Cell Line. WASP was detected in immersion fixed Jurkat human acute T cell leukemia cell line using Goat Anti-Human/Mouse WASP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3070) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: WASP
WASP is a 54 kDa protein belonging to the WASP/SCAR/WAVE family of proteins involved in actin polymerization. It is a highly conserved protein and is expressed selectively in hematopoietic stem cell-derived lineages. WASP contains multiple domains with unique functions. Mutations in WASP are associated with diseases of the hematopoietic/immunologic system.
Product Datasheets
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