Human/Mouse Wnt-10a Antibody

Catalog #: MAB34691 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB34691
MAB34691-SP

Detection of Human and Mouse Wnt‑10a by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse Wnt-10a Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects mouse and human Wnt-10a in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse Wnt-1, -2, -2b, -3a, -4, -5a, -5b, recombinant human (rh) Wnt-6 or rhWnt-7a is observed.

Source

Monoclonal Rat IgG₁ Clone # 342811

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant mouse Wnt-10a
Leu55-Glu95 and Gln278-Arg360
Accession # P70701

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human and Mouse Wnt‑10a by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse Wnt-10a Monoclonal Antibody (Catalog # MAB34691) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Wnt-10a at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Wnt-10a

Wnt-10a is a presumably secreted, glycosylated member of the Wnt family of developmental proteins. Mouse Wnt-10a is synthesized as a 417 amino acid (aa) precursor that contains a 382 aa mature region. The mature region contains 24 cysteines and two potential N-linked glycosylation sites. Mature mouse Wnt-10a shares 96% and 99% aa sequence identity with mature human and rat Wnt-10a, respectively. Wnt-10a shares 64% aa sequence identity with Wnt-10b/12. Wnt-10a is produced by tumors and thymic epithelium.

Long Name

Wingless-type MMTV Integration Site Family Member 10A

Entrez Gene IDs

80326 (Human); 22409 (Mouse); 316527 (Rat)

Alternate Names

FLJ14301; protein Wnt-10a; SSPS; wingless-type MMTV integration site family, member 10A; Wnt10a; Wnt-10a