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Human/Mouse Wnt-5a Antibody

Catalog #: MAB645
20 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB645
MAB645-SP
Wnt‑5a in Mouse Embryo.
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Product Details
Citations (20)
FAQs
Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse Wnt-5a Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects mouse Wnt-5a in direct ELISAs and human and mouse Wnt-5a in Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse Wnt-1, 2b, 3a, 4, 5b, 8a, 8b, 9b, 10a, 10b, 11, or 16 is observed.
Source
Monoclonal Rat IgG2A Clone # 442625
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
CHO-derived recombinant mouse Wnt-5a Gln38-Lys380 Accession # P22725
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Wnt-5a antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr). View Larger

Wnt‑5a in Mouse Embryo. Wnt-5a was detected in immersion fixed frozen sections of mouse embryo using Human/Mouse Wnt-5a Monoclonal Antibody (Catalog # MAB645) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (orange; Catalog # NL013) and counter-stained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Background: Wnt-5a

Wnt proteins are secreted glycoproteins that contain a conserved pattern of 23-24 cysteine residues. Wnts play critical roles in both carcinogenesis and embryonic development for a variety of organisms. Wnts bind to receptors of the Frizzled family, sometimes in conjunction with other membrane-associated proteins such as LRPs or proteoglycans. Downstream effects of Wnt signaling occur through different intracellular components, depending on which pathway is activated. Three pathways have been characterized: the canonical Wnt/ beta -catenin pathway, the Wnt/Ca 2+ pathway, and the planar cell polarity (1-2). Wnt-5a is part of the subgroup of Wnts that are not axis-inducing in Xenopus embryos and do not transform C57MG mammary epithelial cells. This subgroup is also implicated in the Wnt/Ca2+ pathway, playing roles in cell movements and cell adhesion (3). This non-canonical Wnt pathway can inhibit canonical Wnt/ beta -catenin signaling. In Wnt-5a deficient mouse embryos, beta -catenin accumulates in the limb bud suggesting that Wnt-5a normally promotes degradation of beta -catenin (4). Likewise, in Xenopus embryos Wnt-5a antagonizes the ability of the canonical Wnt subgroup to induce a secondary axis (5). Wnt-5a is implicated in various types of cancer and has complex roles. It acts as a tumor suppressor for mammary, B-cell, colon, and uroepithelial cancer cells but is up-regulated in melanomas, where expression levels correlate with severity of metastasis (3). Furthermore, aberrant Wnt-5a signaling results in other diseases such as rheumatoid arthritis (6). Like other developmental growth factors Wnt-5a has diverse roles in development. They are too numerous to enunciate here, as functions span from early anterior-posterior development and gastrulation movements to maintaining hematopoietic stem cell population, lung morphogenesis, and limb outgrowth. Mature Wnt-5a is a 49 kDa protein that shares 99% amino acid identity in mouse, rat and human.

References
  1. Miller, J.R. (2002) Genome Biol. 3:3001.
  2. Roelink, H. and R. Nusse (1991) Genes Dev. 5:381.
  3. Veeman, M.T. et al. (2003) Developmental Cell 5:367.
  4. Topol, L. et al. (2003) J. Cell Biol 162:899.
  5. Torres, M. et al. (1996) J. Cell Biol. 133:1123.
  6. Sen, M. et al. (2001) Arthritis & Rheumatism 44:772.
Long Name
Wingless-type MMTV Integration Site Family, Member 5a
Entrez Gene IDs
7474 (Human); 22418 (Mouse)
Alternate Names
hWNT5A; protein Wnt-5a; wingless-type MMTV integration site family, member 5A; WNT-5A protein; Wnt5a; Wnt-5a

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Citations for Human/Mouse Wnt-5a Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

20 Citations: Showing 1 - 10
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  1. Development of opioid-induced hyperalgesia depends on reactive astrocytes controlled by Wnt5a signaling
    Authors: Liu X, Bae C, Liu B et al.
    Molecular Psychiatry
  2. Increased MARCKS Activity in BRAF Inhibitor-Resistant Melanoma Cells Is Essential for Their Enhanced Metastatic Behavior Independent of Elevated WNT5A and IL-6 Signaling
    Authors: V Yadav, N Jobe, SR Satapathy, P Mohapatra, T Andersson
    Cancers, 2022-12-10;14(24):.
    Species: Human
    Sample Types: Protein Extracts
    Applications: Western Blot
  3. YAP-dependent Wnt5a induction in hypertrophic adipocytes restrains adiposity
    Authors: GJ Lee, YJ Kim, B Park, S Yim, C Park, H Roh, Y Moon, JK Seong, H Park
    Cell Death & Disease, 2022-04-27;13(4):407.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. RNF43 inhibits WNT5A-driven signaling and suppresses melanoma invasion and resistance to the targeted therapy
    Authors: T Radaszkiew, M Nosková, K Gömöryová, O Vondálová, KA Radaszkiew, M Picková, R Víchová, T Gybe?, K Kaiser, L Demková, L Ku?erová, T Bárta, D Pot?šil, Z Zdráhal, K Sou?ek, V Bryja
    Elife, 2021-10-27;10(0):.
    Species: Human, Transgenic Human, Xenograft
    Sample Types: Cell Culture Supernates, Cell Lysates, Tissue Homogenates
    Applications: Western Blot
  5. HDAC6 suppresses microRNA-199a transcription and augments HPV-positive cervical cancer progression through Wnt5a upregulation
    Authors: Y Shao, F Zhu, S Zhu, L Bai
    The international journal of biochemistry & cell biology, 2021-04-29;0(0):106000.
    Species: Human
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC, Western Blot
  6. Myeloid-Derived Suppressor Cells Are a Major Source of Wnt5A in the Melanoma Microenvironment and Depend on Wnt5A for Full Suppressive Activity
    Authors: Douglass SM, Fane ME, Sanseviero E et al.
    Cancer Research
  7. Deletion of Wnt5a in osteoclasts results in bone loss through decreased bone formation
    Authors: Roberts JL, Liu G, Paglia DN et al.
    Annals of the New York Academy of Sciences
  8. Host Wnt5a Potentiates Microenvironmental Regulation of Ovarian Cancer Metastasis
    Authors: M Asem, AM Young, C Oyama, AG Claure De, Y Liu, J Yang, TS Hilliard, J Johnson, EI Harper, I Guldner, S Zhang, TM Page-Maybe, WJ Kaliney, MS Stack
    Cancer Res., 2020-01-13;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Wnt5a causes ROR1 to complex and activate cortactin to enhance migration of chronic lymphocytic leukemia cells.
    Authors: Hasan M, Rassenti L, Widhopf G, Yu J, Kipps T
    Leukemia, 2018-12-19;33(3):653-661.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  10. Distinct roles and differential expression levels of Wnt5a mRNA isoforms in colorectal cancer cells
    Authors: TC Huang, PT Lee, MH Wu, CC Huang, CY Ko, YC Lee, DY Lin, YW Cheng, KH Lee
    PLoS ONE, 2017-08-31;12(8):e0181034.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  11. ABCB1 and ABCG2 drug transporters are differentially expressed in non-small cell lung cancers (NSCLC) and expression is modified by cisplatin treatment via altered Wnt signaling
    Authors: M Vesel, J Rapp, D Feller, E Kiss, L Jaromi, M Meggyes, G Miskei, B Duga, G Smuk, T Laszlo, I Karner, JE Pongracz
    Respir. Res, 2017-03-24;18(1):52.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  12. Noncanonical WNT-5A signaling impairs endogenous lung repair in COPD
    Authors: Hoeke A Baarsma
    J. Exp. Med, 2016-12-15;0(0):.
    Species: Human, Mouse
    Sample Types: In Vivo, Tissue Homogenates
    Applications: Neutralization, Western Blot
  13. WNT5A signaling impairs breast cancer cell migration and invasion via mechanisms independent of the epithelial-mesenchymal transition
    J Exp Clin Cancer Res, 2016-09-13;35(1):144.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  14. Noncanonical Wnt signaling promotes obesity-induced adipose tissue inflammation and metabolic dysfunction independent of adipose tissue expansion.
    Authors: Fuster J, Zuriaga M, Ngo D, Farb M, Aprahamian T, Yamaguchi T, Gokce N, Walsh K
    Diabetes, 2014-10-28;64(4):1235-48.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  15. Stage of breast cancer progression influences cellular response to activation of the WNT/planar cell polarity pathway.
    Authors: MacMillan, Connor D, Leong, Hon S, Dales, David W, Robertson, Amy E, Lewis, John D, Chambers, Ann F, Tuck, Alan B
    Sci Rep, 2014-09-10;4(0):6315.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  16. Wnt5a promotes inflammatory responses via nuclear factor kappaB (NF-kappaB) and mitogen-activated protein kinase (MAPK) pathways in human dental pulp cells.
    Authors: Zhao Y, Wang C, Li R, Hui T, Su Y, Yuan Q, Zhou X, Ye L
    J Biol Chem, 2014-07-25;289(30):21028-39.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  17. Interleukin-6 drives melanoma cell motility through p38alpha-MAPK-dependent up-regulation of WNT5A expression.
    Authors: Linnskog R, Jonsson G, Axelsson L, Prasad C, Andersson T
    Mol Oncol, 2014-05-27;8(8):1365-78.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  18. Wnt5a/beta-catenin signaling drives calcium-induced differentiation of human primary keratinocytes.
    Authors: Popp T, Steinritz D, Breit A, Deppe J, Egea V, Schmidt A, Gudermann T, Weber C, Ries C
    J Invest Dermatol, 2014-03-21;134(8):2183-91.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  19. Oncogenic effects of WNT5A in Epstein-Barr virusassociated nasopharyngeal carcinoma.
    Authors: Yap L, Ahmad M, Zabidi M, Chu T, Chai S, Lee H, Lim P, Wei W, Dawson C, Teo S, Khoo A
    Int J Oncol, 2014-03-13;44(5):1774-80.
    Species: Human
    Sample Types: Protein
    Applications: Western Blot
  20. Diverse mechanisms for activation of Wnt signalling in the ovarian tumour microenvironment.
    Authors: Barbolina MV, Burkhalter RJ, Stack MS
    Biochem. J., 2011-07-01;437(1):1-12.
    Species: Human
    Sample Types: Ascites Fluid
    Applications: Western Blot

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Human/Mouse Wnt-5a Antibody
By Anonymous on 12/09/2020
Application: ICC/IF Sample Tested: E11.5 mouse embryo fixed in 4% PFA Species: Mouse

Staining was done on E11.5 mouse embryo heart sections (Right Ventricle) (4% PFA fixed).
Concentration used - 8ug/mL.

Immunocytochemistry/Immunofluorescence Human/Mouse Wnt-5a Antibody MAB645