Human/Mouse Wnt-8b Antibody

Catalog #: MAB3367 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3367
MAB3367-SP

All Wnt-8b Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse Wnt-8b Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects mouse Wnt-8b in direct ELISAs. In direct ELISAs, no cross‑reactivity with recombinant mouse Wnt-1, -2b, -3a, -4, -5a, -5b, -8a, -9b,
-10b, recombinant human Wnt-2, -7a, -7b, or -9a is observed. Immunohistochemical staining of the human neural cell line, NTera2, demonstrates that this antibody also recognizes human Wnt-8b.

Source

Monoclonal Rat IgG_2A Clone # 341504

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant mouse Wnt‑8b

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

8-25 µg/mL

Immersion fixed frozen sections of embryonic mouse brain

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Wnt-8b

Mouse Wnt-8b is a 40-50 kDa secreted glycoprotein member of the Wnt family of proteins. Mature mouse Wnt-8b is 329 aa in length and contains 24 cysteines plus three potential N-linked glycosylation sites. Little is known about Wnt-8b. It appears to play a role in forebrain development. The mature mouse protein is 100%, 98%, and 97% identical to mature rat, canine, and human Wnt-8b, respectively.

Long Name

Wingless-type MMTV Integration Site Family, Member 8b

Entrez Gene IDs

7479 (Human); 22423 (Mouse); 293990 (Rat)

Alternate Names

protein Wnt-8b; wingless-type MMTV integration site family, member 8B; Wnt8b; Wnt-8b