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Human MUC-1 Antibody

Catalog #: AF6298
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF6298
AF6298-SP
Detection of Human MUC‑1 by Western Blot.
2 Images
Product Details
Citations (3)
FAQs
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human MUC-1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human MUC‑1 in direct ELISAs and Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli‑derived recombinant human MUC-1
Pro126-Arg145
Accession # P15941
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human MUC-1 antibody by Western Blot. View Larger

Detection of Human MUC‑1 by Western Blot. Western blot shows lysates of human lung tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human MUC-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6298) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for MUC-1 at approximately 230 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry MUC-1 antibody in Human Breast by Immunohistochemistry (IHC-P). View Larger

MUC‑1 in Human Breast. MUC-1 was detected in immersion fixed paraffin-embedded sections of human breast using Sheep Anti-Human MUC-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6298) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei and cytoplasm in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MUC-1

MUC-1 (Mucin-1; also episialin, MAM-6, PEM and PUM) is a 150-450 kDa type I transmembrane glycoprotein that belongs to the Mucin family of molecules. It forms a protective barrier against microbes on the apical surface of most glandular epithelium, and becomes prominently displayed on tumor cells. Mature human MUC-1 is 1232 amino acids (aa) in length. It contains an 1135 aa extracellular domain (ECD) (aa 24-1158), and a signal transducing 73 aa cytoplasmic tail (aa 1182-1255). The ECD is highly polymorphic as evidenced by a highly variable number of 20 aa O-glycosylated tandem repeats. Intracellular processing cleaves MUC-1 between Gly1097 and Ser1098, generating a noncovalently linked heterodimer that is expressed on the cell surface. The C-terminus is 22-25 kDa in size, while the N-terminus is highly variable (70-400 kDa in size), a function of the number of resident tandem repeats. Cell surface processing by TACE releases a soluble ECD fragment of variable MW. There are multiple splice variants that show massive reductions in the size of the ECD. Over aa 126-145, human MUC-1 shares < 50% aa identity with mouse MUC-1. When compared to human, mouse MUC-1 is found not to be polymorphic and possess poorly conserved tandem repeat sequences.

Long Name
Mucin 1, Cell Surface-associated
Entrez Gene IDs
4582 (Human)
Alternate Names
Breast carcinoma-associated antigen DF3; Carcinoma-associated mucin; CD227 antigen; CD227; DF3 antigen; EMA; Episialin; H23 antigen; H23AG; KL-6; MAM6; MUC-1; MUC1/ZD; mucin 1, cell surface associated; mucin 1, transmembrane; Mucin-1; Peanut-reactive urinary mucin; PEM; PEMMUC-1/SEC; PEMT; Polymorphic epithelial mucin; PUMMUC-1/X; tumor associated epithelial mucin; Tumor-associated epithelial membrane antigen; Tumor-associated mucin

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Citations for Human MUC-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. IL-22-Activated MUC13 Impacts on Colonic Barrier Function through JAK1/STAT3, SNAI1/ZEB1 and ROCK2/MAPK Signaling
    Authors: Tom Breugelmans, Wout Arras, Baptiste Oosterlinck, Aranzazu Jauregui-Amezaga, Michaël Somers, Bart Cuypers et al.
    Cells
  2. Mucin-microbiome signatures shape the tumor microenvironment in gastric cancer
    Authors: Oosterlinck B, Ceuleers H, Arras W et al.
    Microbiome
  3. A dynamic mucin mRNA signature associates with COVID-19 disease presentation and severity
    Authors: A Smet, T Breugelman, J Michiels, K Lamote, W Arras, JG De Man, L Heyndrickx, A Hauner, M Huizing, S Malhotra-K, M Lammens, A Hotterbeek, S Kumar-Sing, A Verstraete, B Loeys, V Verhoeven, R Jacobs, K Dams, S Coenen, KK Ariën, PG Jorens, BY De Winter
    JCI Insight, 2021-10-08;6(19):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC

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