Human MuSK Antibody Summary
Glu22-Ser503
Accession # O15146
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MuSK
MuSK (muscle-specific kinase) is a 100 kDa type I transmembrane (TM) protein belonging to the receptor tyrosine kinase family. It is found in the postsynaptic membrane of skeletal muscle motor endplates. MuSK does not bind agrin directly, but is part of an agrin receptor complex that promotes the clustering of acetylcholine receptor at the neuromuscular junction (NMJ). Over residues 22‑503, human MuSK shares 92% amino acid sequence identity with mouse MuSK. Human MuSK apparently has multiple isoforms. One contains deletions at residues 307‑394 and 454‑461, while a second is a short soluble form that contains residues 120‑209 plus a unique 24 aa C‑terminal tail.
Product Datasheets
Citation for Human MuSK Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
-
Dok-7 regulates neuromuscular synapse formation by recruiting Crk and Crk-L.
Authors: Hallock PT, Xu CF, Park TJ
Genes Dev., 2010-11-01;24(21):2451-61.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Human MuSK Antibody
Average Rating: 3 (Based on 2 Reviews)
Have you used Human MuSK Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Filter by:
C2C12 differentiated myotubes, following IP with an anti-MuSK antibody (different from this Ab), SDS-PAGE, and transferred into PVDF membrane. After blocking with 5% BSA/TBST, blot was probed with 1:400 of AF562 (4C, o/n). Subsequent probing with a HRP-anti-goat secondary Ab yielded excellent publishable results, with 100 kDa MuSK band visible.
Differentiated C2C12 mouse skeletal muscle lysates IP'ed for MuSK using a donated Ab. IP works well and I can detect phosphorylation of MuSK using a general phosphotyrosine Ab, but upon stripping, blocking, and probing with this Ab, I am unable to detect MuSK (perhaps due to high background signal). Probing with a different MuSK Ab is much cleaner
Technical Service is following up
