Human MyD88 Antibody

Detection of Human MyD88 by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line, Jurkat human acute T cell leukemia cell line, and HT-29 human colon adenocarcinoma cell line. PVDF membrane was probed with 0.5 µg/mL Goat Anti-Human MyD88 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2928) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). For additional reference, recombinant human MyD88 (1 ng) was included. A specific band for MyD88 was detected at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

MyD88 in Raji Human Cell Line. MyD88 was detected in immersion fixed Raji human Burkitt's lymphoma cell line using Goat Anti-Human MyD88 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2928) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Detection of MyD88 in Raji Human Cell Line by Flow Cytometry. Raji human Burkitt's lymphoma cell line was stained with Goat Anti-Human MyD88 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2928, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Detection of Human MyD88 by Simple Western^TM. Simple Western lane view shows lysates of Raji human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for MyD88 at approximately 41 kDa (as indicated) using 5 µg/mL of Goat Anti-Human MyD88 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2928) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Western Blot Shows Human MyD88 Specificity by Using Knockout Cell Line. Western blot shows lysates of Raji human Burkitt's lymphoma cell line and human MyD88 knockout Raji human Burkitt's lymphoma cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human MyD88 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2928) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for MyD88 at approximately 35 kDa (as indicated) in the parental Raji human Burkitt's lymphoma cell line, but is not detectable in knockout Raji human Burkitt's lymphoma cell line. GAPDH (AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.