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Human Myeloperoxidase Quantikine ELISA Kit

Catalog #: DMYE00
35 Citations 2 Reviews Datasheet / COA / SDS
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Human Myeloperoxidase/MPO ELISA Cell Culture Supernate/Cell Lysate/Saliva Standard Curve
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Product Details
Procedure
Citations (35)
FAQs
Reviews (2)
Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human Myeloperoxidase Quantikine ELISA Kit Summary

Assay Length
3.5 hours or 4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Cell Lysates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Saliva (10 uL), Urine (50 uL), Human Milk (13 uL)
Sensitivity
0.618 ng/mL
Assay Range
1.6 - 100 ng/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk)
Specificity
Natural and recombinant human Myeloperoxidase
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human Myeloperoxidase Immunoassay is a 3.5 or 4.5 hour solid phase ELISA designed to measure human Myeloperoxidase in cell culture supernates, cell lysates, serum, plasma, saliva, urine, and human milk. It contains NS0-expressed recombinant human Myeloperoxidase and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Myeloperoxidase showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring Myeloperoxidase.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Cell Lysates, Saliva, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (ng/mL) 10.3 20.4 39.4 10.3 21.7 42.8
Standard Deviation 0.75 1.5 3.6 1 1.9 4
CV% 7.2 7.6 9.1 10 8.8 9.2

Serum, EDTA Plasma, Heparin Plasma, Human Milk

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (ng/mL) 15.7 32.4 64.1 19 38.3 69.2
Standard Deviation 1.2 2.5 4.3 1.8 3.2 4.5
CV% 7.5 7.7 6.6 9.4 8.3 6.5

Recovery

The recovery of Myeloperoxidase spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 104 88-119

Linearity

To assess the linearity of the assay, samples containing high concentrations of Myeloperoxidase were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human Myeloperoxidase/MPO ELISA Linearity

Scientific Data

N/A Human Myeloperoxidase/MPO ELISA Cell Culture Supernate/Cell Lysate/Saliva Standard Curve View Larger

Human Myeloperoxidase/MPO ELISA Cell Culture Supernate/Cell Lysate/Saliva Standard Curve

N/A Human Myeloperoxidase/MPO ELISA Serum/Plasma/Urine Standard Curve View Larger

Human Myeloperoxidase/MPO ELISA Serum/Plasma/Urine Standard Curve

Product Datasheets

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Product Datasheet
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Preparation and Storage

Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: Myeloperoxidase/MPO

Myeloperoxidase (MPO) is a heme-containing enzyme belonging to the XPO subfamily of peroxidases. It is an abundant neutrophil and monocyte glycoprotein that catalyzes the hydrogen peroxide dependent formation of hypochlorus acid (HOCl) and other reactive species. Enzymatically active MPO is a disulfide-linked tetramer that contains two heme groups and two copies each of the heavy and light chains. MPO binds Albumin, MMR, Cytokeratin 1 on vascular endothelial cells, HMW Kininogen, and Integrin CD11b/CD18 on neutrophils. These interactions promote MPO clearance, a reduction of nitric oxide and bradykinin levels, reduced vasodilation, and continued neutrophil activation. Neutrophil MPO is stored in cytoplasmic azurophilic granules. Upon cellular activation and degranulation, MPO is delivered into phagosomes where it is required for the killing of phagocytosed bacteria. Activated neutrophils also release granule contents extracellularly. Elevated plasma MPO levels have been associated with a variety of clinical conditions including systemic inflammation, eclampsia, risk of cardiovascular events, vascular endothelial dysfunction, severity of multiple sclerosis, and prospective mortality and oxidative stress during hemodialysis.

Entrez Gene IDs:
4353 (Human); 17523 (Mouse); 303413 (Rat)
Alternate Names:
EC 1.11.1; EC 1.11.1.7; MPO; Myeloperoxidase

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. Add Assay Diluent
  3.   For Serum, Plasma, & Human Milk Samples: Add 50 µL of Assay Diluent to each well.
    For Cell Culture Supernate, Cell Lysate, Saliva, & Urine Samples: Add 100 µL Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well.
  7.   For Serum, Plasma, & Human Milk Samples: Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
    For Cell Culture Supernate, Cell Lysate, Saliva, & Urine Samples: Cover with a new plate sealer, and incubate at room temperature for 1 hour on the shaker.
  8.   Aspirate and wash 4 times.
    9. 200 µL Substrate Solution
  9.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 20 minutes on the benchtop. PROTECT FROM LIGHT.
    10. 50 µL Stop Solution
  10. Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human Myeloperoxidase Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

35 Citations: Showing 1 - 10
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  1. Blood concentrations of mediators released from activated neutrophils are related to the severity of alcohol-induced liver damage
    Authors: B Kasztelan-, B Zygo, A Rycyk-Boja, A Surdacka, J Rolinski, H Cichoz-Lac
    PLoS ONE, 2023-01-06;18(1):e0280068.
    Species: Human
    Sample Types: Serum
  2. NETosis and Nucleosome Biomarkers in Septic Shock and Critical COVID-19 Patients: An Observational Study
    Authors: L Morimont, M Dechamps, C David, C Bouvy, C Gillot, H Haguet, J Favresse, L Ronvaux, J Candiracci, M Herzog, PF Laterre, J De Poorter, S Horman, C Beauloye, J Douxfils
    Biomolecules, 2022-07-27;12(8):.
    Species: Human
    Sample Types: Plasma
  3. Rare germline alterations of myeloperoxidase predispose to myeloid neoplasms
    Authors: S Kongkiatka, L Terkawi, Y Guan, V Adema, M Hasipek, T Dombrovski, M Co, W Walter, H Awada, Y Parker, S Hutter, S Pagliuca, C Gurnari, HJ Rogers, M Meggendorf, DJ Lindner, T Haferlach, V Visconte, T LaFrambois, BK Jha, JP Maciejewsk
    Leukemia, 2022-06-27;0(0):.
    Species: Human
    Sample Types: Cell Lysates
  4. Cell-Free DNA, Neutrophil extracellular traps (NETs), and Endothelial Injury in Coronavirus Disease 2019- (COVID-19-) Associated Acute Kidney Injury
    Authors: BM Henry, MHS de Oliveir, I Cheruiyot, J Benoit, J Rose, EJ Favaloro, G Lippi, S Benoit, N Pode Shakk
    Mediators of Inflammation, 2022-04-22;2022(0):9339411.
    Species: Human
    Sample Types: Plasma
  5. Tumour cell apoptosis modulates the colorectal cancer immune microenvironment via interleukin-8-dependent neutrophil recruitment
    Authors: V Schimek, K Strasser, A Beer, S Göber, N Walterskir, C Brostjan, C Müller, T Bachleitne, M Bergmann, H Dolznig, R Oehler
    Cell Death & Disease, 2022-02-04;13(2):113.
    Species: Human
    Sample Types: Cell Culture Supernates
  6. Association of Lipoproteins with Neutrophil Extracellular Traps in Patients with Abdominal Aortic Aneurysm
    Authors: A Brandau, N Ibrahim, J Klopf, H Hayden, M Ozsvar-Koz, T Afonyushki, S Bleichert, L Fuchs, V Watzinger, V Nairz, E Manville, V Kessler, H Stangl, W Eilenberg, C Neumayer, C Brostjan
    Biomedicines, 2022-01-20;10(2):.
    Species: Human
    Sample Types: Plasma
  7. Association of circulating MR-proADM with all-cause and cardiovascular mortality in the general population: Results from the KORA F4 cohort study
    Authors: C Gar, B Thorand, C Herder, C Sujana, M Heier, C Meisinger, A Peters, W Koenig, W Rathmann, M Roden, M Stumvoll, H Maalmi, T Meitinger, H Then, J Seissler, C Then
    PLoS ONE, 2022-01-06;17(1):e0262330.
    Species: Human
    Sample Types: Serum
  8. NETs biomarkers in saliva and serum OSCC patients: One hypothesis, two conclusions
    Authors: M Garley, D Dziemia?cz, W Ratajczak-, A Pryczynicz, K Nowak, B ?azarczyk, E Jab?o?ska
    Advances in medical sciences, 2021-12-28;67(1):45-54.
    Species: Human
    Sample Types: Saliva
  9. Role of Clinical Characteristics and Biomarkers at Admission to Predict One-Year Mortality in Elderly Patients with Pneumonia
    Authors: A Malézieux-, L Azurmendi, S Pagano, N Vuilleumie, JC Sanchez, D Zekry, JL Reny, J Stirnemann, N Garin, V Prendki, On Behalf
    Journal of Clinical Medicine, 2021-12-25;11(1):.
    Species: Human
    Sample Types: Plasma
  10. Association between Biomarkers of Oxidative Stress and Inflammation with Cardiac Necrosis and Heart Failure in Non-ST Segment Elevation Myocardial Infarction Patients and Various Degrees of Kidney Function
    Authors: S Roumelioti, A Veljkovic, PI Georgianos, G Lazarevic, Z Perisic, J Hadzi-Djok, V Liakopoulo, G Kocic
    Oxidative Medicine and Cellular Longevity, 2021-11-01;2021(0):3090120.
    Species: Human
    Sample Types: Serum
  11. ELISA detection of MPO-DNA complexes in human plasma is error-prone and yields limited information on neutrophil extracellular traps formed in vivo
    Authors: H Hayden, N Ibrahim, J Klopf, B Zagrapan, LM Mauracher, L Hell, TM Hofbauer, AS Ondracek, C Schoergenh, B Jilma, IM Lang, I Pabinger, W Eilenberg, C Neumayer, C Brostjan
    PLoS ONE, 2021-04-22;16(4):e0250265.
    Species: Human
    Sample Types: Plasma
  12. Profile of new vascular damage biomarkers in middle-aged men with arterial hypertension
    Authors: AE Charkiewic, M Garley, W Ratajczak-, K Nowak, E Jab?o?ska, D Ma?lach, WJ Omeljaniuk
    Advances in medical sciences, 2021-03-05;66(1):185-191.
    Species: Human
    Sample Types: Serum
  13. Characterization of ADAMTS13 and von Willebrand factor levels in septic and non-septic ICU patients
    Authors: K Singh, AC Kwong, H Madarati, S Kunasekara, T Sparring, AE Fox-Robich, PC Liaw, CA Kretz
    PLoS ONE, 2021-02-19;16(2):e0247017.
    Species: Human
    Sample Types: Plasma
  14. Granulocyte and monocyte adsorptive apheresis induces apoptosis of neutrophils and release of a novel chemoattractant for desensitization of interleukin-8 response - In vitro and in vivo results
    Authors: N Kashiwagi, F Saito, H Maegawa, K Kaneda
    Cytokine, 2021-01-01;139(0):155410.
    Species: Human
    Sample Types: Plasma
  15. Resveratrol and exercise combined to treat functional limitations in late life: A pilot randomized controlled trial
    Authors: SA Harper, JR Bassler, S Peramsetty, Y Yang, LM Roberts, D Drummer, RT Mankowski, C Leeuwenbur, K Ricart, RP Patel, MM Bamman, SD Anton, BC Jaeger, TW Buford
    Exp Gerontol, 2020-10-15;0(0):111111.
    Species: Human
    Sample Types: Plasma
  16. The role of specific biomarkers, as predictors of post-operative complications following flexible ureterorenoscopy (FURS), for the treatment of kidney stones: a single-centre observational clinical pilot-study in 37 patients
    Authors: SF Hughes, AJ Moyes, RM Lamb, P Ella-Tongw, C Bell, A Moussa, I Shergill
    BMC Urol, 2020-08-14;20(1):122.
    Species: Human
    Sample Types: Plasma, Serum
  17. Biomarkers of neutrophil extracellular traps (NETs) and nitric oxide-(NO)-dependent oxidative stress in women who miscarried
    Authors: WJ Omeljaniuk, E Jab?o?ska, M Garley, A Pryczynicz, W Ratajczak-, K Socha, MH Borawska, AE Charkiewic
    Sci Rep, 2020-08-04;10(1):13088.
    Species: Human
    Sample Types: Serum
  18. Neutrophil Phenotypes in Coronary Artery Disease
    Authors: P Maréchal, J Tridetti, ML Nguyen, O Wéra, Z Jiang, M Gustin, AF Donneau, C Oury, P Lancellott
    J Clin Med, 2020-05-25;9(5):.
    Species: Human
    Sample Types: Plasma
  19. Neutrophil activation and NETosis are the major drivers of thrombosis in heparin-induced thrombocytopenia
    Authors: J Perdomo, HHL Leung, Z Ahmadi, F Yan, JJH Chong, FH Passam, BH Chong
    Nat Commun, 2019-03-21;10(1):1322.
    Species: Human
    Sample Types: Plasma
  20. Neutrophil Dysfunction in the Airways of Children with Acute Respiratory Failure Due to Lower Respiratory Tract Viral and Bacterial Coinfections
    Authors: JR Grunwell, VD Giacalone, S Stephenson, C Margaroli, BS Dobosh, MR Brown, AM Fitzpatric, R Tirouvanzi
    Sci Rep, 2019-02-27;9(1):2874.
    Species: Human
    Sample Types: Tracheal Aspirate
  21. Cardiac magnetic resonance using late gadolinium enhancement and atrial T1 mapping predicts poor outcome in patients with atrial fibrillation after catheter ablation therapy
    Authors: JA Luetkens, AC Wolpers, T Beiert, D Kuetting, D Dabir, R Homsi, H Meenderman, NA Dayé, V Knappe, M Karsdal, SH Nielsen, F Genovese, F Stöckigt, M Linhart, D Thomas, G Nickenig, HH Schild, JW Schrickel, RP Andrié
    Sci Rep, 2018-09-11;8(1):13618.
    Species: Human
    Sample Types: Serum
  22. Myeloperoxidase level and inflammatory markers and lipid and lipoprotein parameters in stable coronary artery disease
    Authors: E Kimak, B Zi?ba, D Duma, J Solski
    Lipids Health Dis, 2018-04-04;17(1):71.
    Species: Human
    Sample Types: Serum
  23. Citrullinated histone H3 as a novel prognostic blood marker in patients with advanced cancer
    Authors: C Thålin, S Lundström, C Seignez, M Daleskog, A Lundström, P Henriksson, T Helleday, M Phillipson, H Wallén, M Demers
    PLoS ONE, 2018-01-11;13(1):e0191231.
    Species: Human
    Sample Types: Plasma
  24. Paraoxonase-1 and myeloperoxidase correlate with vascular biomarkers in overweight patients with newly diagnosed untreated hyperlipidaemia
    Authors: A Szentpéter, N Zsíros, VE Varga, H L?rincz, M Katkó, I Seres, P Fülöp, G Paragh, M Harangi
    VASA, 2017-06-12;0(0):1-7.
    Species: Human
    Sample Types: Serum
  25. Inflammatory Differences in Plaque Erosion and Rupture in Patients With ST-Segment Elevation Myocardial Infarction
    Authors: S Chandran, J Watkins, A Abdul-Aziz, M Shafat, PA Calvert, KM Bowles, MD Flather, SA Rushworth, AD Ryding
    J Am Heart Assoc, 2017-05-03;6(5):.
    Species: Human
    Sample Types: Plasma
  26. The interdependencies of viral load, the innate immune response, and clinical outcome in children presenting to the emergency department with respiratory syncytial virus-associated bronchiolitis
    Authors: FA Piedra, M Mei, V Avadhanula, R Mehta, L Aideyan, RP Garofalo, PA Piedra
    PLoS ONE, 2017-03-07;12(3):e0172953.
    Species: Human
    Sample Types: Nasal Lavage Fluid
  27. Complex regulation of neutrophil-derived MMP-9 secretion in central nervous system tuberculosis
    Authors: CW Ong, PJ Pabisiak, S Brilha, P Singh, F Roncaroli, PT Elkington, JS Friedland
    J Neuroinflammation, 2017-02-07;14(1):31.
    Species: Human
    Sample Types: Whole Cells
  28. Combined administration of anisodamine and neostigmine rescued acute lethal crush syndrome through ?7nAChR-dependent JAK2-STAT3 signaling
    Sci Rep, 2016-11-22;6(0):37709.
    Species: Mouse
    Sample Types: Serum
  29. Inflammatory thresholds and the species-specific effects of colonising bacteria in stable chronic obstructive pulmonary disease.
    Authors: Singh R, Mackay A, Patel A, Garcha D, Kowlessar B, Brill S, Donnelly L, Barnes P, Donaldson G, Wedzicha J
    Respir Res, 2014-09-14;15(0):114.
    Species: Human
    Sample Types: Cell Culture Supernates
  30. Growth differentiation factor-15 (GDF-15) levels are associated with cardiac and renal injury in patients undergoing coronary artery bypass grafting with cardiopulmonary bypass.
    Authors: Kahli A, Guenancia C, Zeller M, Grosjean S, Stamboul K, Rochette L, Girard C, Vergely C
    PLoS ONE, 2014-08-29;9(8):e105759.
    Species: Human
    Sample Types: Plasma
  31. Increased levels of leukocyte-derived MMP-9 in patients with stable angina pectoris.
    Authors: Jonasson S, Lundberg A, Kalvegren H, Bergstrom I, Szymanowski A, Jonasson L
    PLoS ONE, 2011-04-29;6(4):e19340.
    Species: Human
    Sample Types: Serum
  32. Presence of IL-5 protein and IgE antibodies to staphylococcal enterotoxins in nasal polyps is associated with comorbid asthma.
    Authors: Bachert C, Zhang N, Holtappels G, De Lobel L, Van Cauwenberge P, Liu S, Lin P, Bousquet J, Van Steen K
    J. Allergy Clin. Immunol., 2010-11-01;126(5):962.
    Species: Human
    Sample Types: Tissue Homogenates
  33. Interleukin-8 is increased in the membrane of circulating erythrocytes in patients with acute coronary syndrome.
    Authors: Tziakas DN, Chalikias GK, Tentes IK, Stakos D, Chatzikyriakou SV, Mitrousi K, Kortsaris AX, Kaski JC, Boudoulas H
    Eur. Heart J., 2008-09-02;29(22):2713-22.
    Species: Human
    Sample Types: Plasma
  34. Effects of atorvastatin added to inhaled corticosteroids on lung function and sputum cell counts in atopic asthma.
    Authors: Hothersall EJ, Chaudhuri R, McSharry C, Donnelly I, Lafferty J, McMahon AD, Weir CJ, Meiklejohn J, Sattar N, McInnes I, Wood S, Thomson NC
    Thorax, 2008-08-29;63(12):1070-5.
    Species: Human
    Sample Types: Sputum
  35. Computed tomographic scan-diagnosed chronic obstructive pulmonary disease-emphysema: eotaxin-1 is associated with bronchodilator response and extent of emphysema.
    Authors: Miller M, Ramsdell J, Friedman PJ, Cho JY, Renvall M, Broide DH
    J. Allergy Clin. Immunol., 2007-11-01;120(5):1118-25.
    Species: Human
    Sample Types: BALF

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Reviews for Human Myeloperoxidase Quantikine ELISA Kit

Average Rating: 4.5 (Based on 2 Reviews)

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Human Myeloperoxidase Quantikine ELISA Kit
By Anonymous on 09/11/2017
Sample Tested: Blood monocytes
Human Myeloperoxidase Quantikine ELISA Kit
By Anonymous on 09/07/2017
Sample Tested: Peripheral blood neutrophils