Human N-Cadherin APC-conjugated Antibody Summary
Asp160-Ala724
Accession # P19022
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of N‑Cadherin in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Sheep Anti-Human N-Cadherin APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB6426A, filled histogram) or isotype control antibody (Catalog # IC016A, open histogram). View our protocol for Staining Membrane-associated Proteins.
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N-Cadherin Specificity is Shown by Flow Cytometry in Knockout Cell Line N-Cadherin knockout HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human N-Cadherin APC-conjugated Monoclonal Antibody (Catalog # FAB6426A, filled histogram) or isotype control antibody (Catalog # IC016A, open histogram). No staining in the N-Cadherin knockout HeLa cell line was observed. View our protocol for Staining Membrane-associated Proteins.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: N-Cadherin
N-Cadherin (Neural Cadherin; also CD325 and Cadherin-2) is a 130-135 kDa member of the "classical" (or type I) cadherin subfamily, cadherin superfamily of proteins. It is expressed on multiple cell types, including neurons, fibroblasts, Schwann cells, endothelial cells and hepatic stellate cells. N-Cadherin mediates homotypic binding, either in cis (same cell) or trans (adjacent cell). proN-Cadherin is expressed as an 881 amino acid (aa) type I transmembrane glycoprotein. It may be initially inserted into the ER, where the 15-20 kDa prodomain (aa 26-159) is cleaved by proprotein convertase, and the mature molecule (aa 160-906) is transported to the surface. Mature N-Cadherin contains a 565 aa extracellular region (aa 160-724) that possesses five cadherin domains (aa 160-714), and a 161 aa cytoplasmic tail that undergoes phosphorylation at Tyr785. There is one splice variant that contains a 10 aa substitution for aa 839-906. Over aa 160-724, human N Cadherin shares 98% aa identity with mouse N-Cadherin.
Product Datasheets
Citation for Human N-Cadherin APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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The p63 protein isoform DeltaNp63alpha inhibits epithelial-mesenchymal transition in human bladder cancer cells: role of MIR-205.
Authors: Tran M, Choi W, Wszolek M, Navai N, Lee I, Nitti G, Wen S, Flores E, Siefker-Radtke A, Czerniak B, Dinney C, Barton M, McConkey D
J Biol Chem, 2012-12-13;288(5):3275-88.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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