Human NAALADase-like 2/NAALADL2 Antibody

Catalog #: AF4665 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4665
AF4665-SP

Detection of NAALADase‑like 2/ NAALADL2 in LnCAP Human Cell Line by Flow Cytometry.

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Human NAALADase-like 2/NAALADL2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human NAALADase-like 2/NAALADL2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than
5% cross‑reactivity with recombinant mouse NAALADase 2 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human NAALADase-like 2/NAALADL2
Ser152-Asn795 (Met194Thr)
Accession # CAE75743

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human NAALADase-like 2/NAALADL2

Flow Cytometry

2.5 µg/10⁶ cells

See below

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human NAALADase-like 2/
NAALADL2, see our available Western blot detection antibodies

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

5-15 µg/mL

Immersion fixed PC-3 human prostate cancer cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of NAALADase‑like 2/ NAALADL2 in LnCAP Human Cell Line by Flow Cytometry. LnCAP human prostate cancer cell line was stained with Goat Anti-Human NAALADase-like 2/NAALADL2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4665, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoe-rythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NAALADase-like 2/NAALADL2

NAALADL2 (N-acetylated alpha -linked acidic dipeptidase like 2) is a member of the peptidase M28 family of enzymes. It is presumably a type II transmembrane (TM) protein that may have O-glycosyl hydrolase activity. Human NAALADL2 is 795 amino acids (aa) in length. It is believed to contain a cytoplasmic and TM segment at the N-terminus, followed by a peptidase domain (aa 444‑596) and a TfR-like dimerization region (aa 688‑777). There are multiple splice forms. One shows a 4 aa substitution for aa 292‑795, a second shows an alternate start site at Met18 with a 24 aa substitution for aa 314‑795, and a third contains an alternate start site at Met283, accompanied by a deletion of aa 363‑411 and 633‑795. Over aa 152‑795, human NAADADL2 is 87% and 82% aa identical to mouse and canine NAADADL2, respectively.

Long Name

N-Acetylated Alpha-Linked Acidic Dipeptidase-like 2

Entrez Gene IDs

254827 (Human); 635702 (Mouse); 499583 (Rat)

Alternate Names

inactive N-acetylated-alpha-linked acidic dipeptidase-like protein 2; NAALADase L2; NAALADase like 2; NAALADase-like 2; NAALADL2; N-acetylated alpha-linked acidic dipeptidase 2; N-acetylated alpha-linked acidic dipeptidase-like 2