Human Nectin-2/CD112 Antibody Summary
Gln32-Leu360
Accession # NP_002847
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Nectin‑2/CD112 by Western Blot. Western blot shows lysates of 293T human embryonic kidney cell line, A549 human lung carcinoma cell line, Daudi human Burkitt's lymphoma cell line, and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human Nectin-2/CD112 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2229) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Nectin-2/CD112 at approximately 60-75 kDa (as indicated). Daudi human Burkitt's lymphoma cell line and Raji human Burkitt's lymphoma cell line are shown as negative controls. GAPDH (Catalog # AF5718) is shown as a loading control.This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Nectin‑2/CD112 in MCF‑7 Human Cell Line. Nectin-2/CD112 was detected in immersion fixed MCF-7 human breast cancer cell line using Goat Anti-Human Nectin-2/CD112 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2229) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Nectin‑2/CD112 in Human Liver. Nectin-2/CD112 was detected in immersion fixed paraffin-embedded sections of human liver using Goat Anti-Human Nectin-2/CD112 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2229) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
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Detection of Human Nectin‑2/CD112 by Simple WesternTM. Simple Western lane view shows lysates of HEK293T human embryonic kidney cell line, A549 human lung carcinoma cell line, Daudi human Burkitt's lymphoma cell line, and Raji human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Nectin-2/CD112 at approximately 71-86 kDa (as indicated) using 20 µg/mL of Goat Anti-Human Nectin-2/CD112 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2229) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Nectin-2/CD112
Nectins are a small family of Ca++-independent immunoglobulin (Ig)-like cell adhesion molecules (CAMs) that organize intercellular junctions (1). The nectin family has at least four members (nectin-1-4), all of which show alternate splicing (except for Nectin-4), a transmembrane (TM) region (except for Nectin-1 gamma ), and three extracellular Ig-domains. Nectins are highly homologous to the human receptor for poliovirus, and as such have been alternately named poliovirus receptor-related proteins. They do not, however, appear to bind poliovirus (1). Nectin-2 is a 60 or 65 kDa type I TM glycoprotein that is found on a variety of cell types (2, 3). It has two splice forms (4, 5). Nectin-2δ is a 65 kDa long form and is synthesized as a 538 amino acid precursor. It contains a 31 amino acid (aa) signal sequence, a 329 aa extracellular region, a 21 aa TM segment, and a 157 aa cytoplasmic domain. The extracellular region contains one N-terminal 85 aa V-type Ig domain and two 45-55 aa C2-type Ig domains. The V-domain is believed to mediate nectin binding to its ligands (6). The short, 60 kDa isoform of Nectin-2 (Nectin-2 alpha ) has the same signal sequence and extracellular domain as nectin-2δ, but differs in the TM and cytoplasmic region (4, 5). In this case, the cytoplasmic tail is only 94 aa in length. The human extracellular region shows 72% aa sequence identity with the equivalent region in mouse. Nectin-2 is known to bind the pseudorabies virus, and herpes simplex virus-2 (HSV-2), but not HSV-1. It does not bind poliovirus. As a cell adhesion molecule, Nectin-2 will form cis-homodimers (same cell), followed by trans-dimers (across cells). Nectin-2 will not cis-dimerize with other nectins, but will cis-dimerize with its two splice forms. Notably, a Nectin-2 cis-dimer on one cell will heterodimerize with a Nectin-3 cis-dimer on another cell (1). Nectin-2 is found concentrated in adherens junctions, and exists on neurons, endothelial cells, epithelial cells and fibroblasts.
- Takai, Y. and H. Nakanishi, 2003, J. Cell Sci. 116:17.
- Bottino, C. et al. (2003) J. Exp. Med. 198:557.
- Pende, D. et al. (2005) Mol. Immunol. 42:463.
- Eberle, F. et al. (1995) Gene 159:267.
- Warner, M.S. et al. (1998) Virology 246:179.
- Struyf, F. et al. (2002) J. Virol. 76:12940.
Product Datasheets
Citations for Human Nectin-2/CD112 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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CRISPR activation screen identifies BCL-2 proteins and B3GNT2 as drivers of cancer resistance to T cell-mediated cytotoxicity
Authors: J Joung, PC Kirchgatte, A Singh, JH Cho, SP Nety, RC Larson, RK Macrae, R Deasy, YY Tseng, MV Maus, F Zhang
Nature Communications, 2022-03-25;13(1):1606.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Blockade of checkpoint receptor PVRIG unleashes anti-tumor immunity of NK cells in murine and human solid tumors
Authors: Y Li, Y Zhang, G Cao, X Zheng, C Sun, H Wei, Z Tian, W Xiao, R Sun, H Sun
Journal of hematology & oncology, 2021-06-26;14(1):100.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
The Ubiquitin-proteasome pathway regulates Nectin2/CD112 expression and impairs NK cell recognition and killing
Authors: R Molfetta, ND Milito, B Zitti, M Lecce, C Fionda, M Cippitelli, A Santoni, R Paolini
Eur. J. Immunol., 2019-03-27;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation, Western Blot -
Blockade of the checkpoint receptor TIGIT prevents NK cell exhaustion and elicits potent anti-tumor immunity
Authors: Q Zhang, J Bi, X Zheng, Y Chen, H Wang, W Wu, Z Wang, Q Wu, H Peng, H Wei, R Sun, Z Tian
Nat. Immunol., 2018-06-18;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Low-dose bortezomib increases the expression of NKG2D and DNAM-1 ligands and enhances induced NK and ?? T cell-mediated lysis in multiple myeloma
Authors: C Niu, H Jin, M Li, S Zhu, L Zhou, F Jin, Y Zhou, D Xu, J Xu, L Zhao, S Hao, W Li, J Cui
Oncotarget, 2017-01-24;8(4):5954-5964.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
p120 regulates endothelial permeability independently of its NH2 terminus and Rho binding.
Authors: Herron CR, Lowery AM, Hollister PR, Reynolds AB, Vincent PA
Am. J. Physiol. Heart Circ. Physiol., 2010-10-22;300(1):H36-48.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot
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