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Human Neuregulin-1/NRG1 Antibody

Catalog #: MAB377
18 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB377-SP
MAB377-100
MAB377-500
Product Details
Citations (18)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Neuregulin-1/NRG1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Neuregulin‑1/NRG1 in direct ELISAs and Western blots. In direct ELISAs, no cross‑reactivity with the EGF‑like domains of recombinant human (rh) NRG1-beta 1 (aa 176‑246) or rhNRG1-alpha (aa 177‑241). Therefore, this antibody recognizes an epitope located within aa 2‑176 segment N‑terminal to the EGF‑like domain. This segment is present in all NRG1‑ alpha, beta and gamma isoforms and in NDF43 and glial growth factor (GGF). It is absent from the NRG1 gene products GGF2 and SMDF.
Source
Monoclonal Mouse IgG2B Clone # 147724
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Neuregulin‑1/NRG1
Ser2-Lys246
Accession # AAA58639
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Neuregulin‑1/NRG1 (Catalog # 5898-NR)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neuregulin-1/NRG1

The Neuregulin family of structurally related glycoproteins comprises products from four distinct but related genes, Nrg-1, Nrg-2, Nrg-3, and Nrg-4. Through alternative splicing or the use of alternative promoters, Nrg-1 has been shown to encode more than 14 soluble or transmembrane proteins. The extracellular domain of the transmembrane NRG1 isoforms can be proteolytically cleaved to release soluble growth factors. All NRG1 isoforms contain an EGF-like domain ( alpha - or beta -splice variant that differ in their C-terminal region) that is required for their direct binding to the ErbB3 or ErbB4 receptor tyrosine kinases. The ErbB3 or ErbB4 subsequently recruits and heterodimerizes with ErbB2, resulting in tyrosine phosphorylation and NRG1 signaling. NRG1 isoforms can be classified into three major subtypes. Type I (Neu Differentiation Factor, NDF; Heregulin, HRG; Acetylcholine Receptor Inducing Activity, ARIA) and type II (Glial Growth Factor, GGF) NRG1s have an immunoglobulin (Ig)-like domain N-terminal to the EGF-like domain. Type I NRG1s differ from type II NRG1s by having a glycosylation-rich domain between the Ig-like and the EGF-like domains. Type III NRG1s (Sensory and Motor neuron-Derived Factor) lacks the Ig-like domain but has a cysteine rich domain (CRD) instead. NRG1 isoforms show distinct spatial and temporal expression patterns. These proteins play important roles during development of both the nervous system and the heart. They have been shown to regulate the selective expression of neurotransmitter receptors in neurons and at the neuromuscular junction, and promote the differentiation and development of Schwann cells from neural crest stem cells. NRG1s have also been shown to be involved in the establishment of the oligodendroglial lineage.

References
  1. Buonanno, A., and G.D Fischbach (2001) Curr. Opin. Neurobiol. 11:287.
  2. Adlkofer, K. and C. Lai (2000) Glia 29:104.
  3. Garratt, A.N. et al., (2000) BioEssays 22:987.
Entrez Gene IDs
3084 (Human); 211323 (Mouse); 112400 (Rat)
Alternate Names
ARIA; GGF2; GGFglial growth factor; Heregulin-1; HGL; HGLneu differentiation factor; HRG; HRG1; HRG1-alpha; HRG1-beta 1; HRGAneuregulin 1 type IV beta 1a; MST131; MSTP131; NDFheregulin, alpha (45kD, ERBB2 p185-activator); neuregulin 1 type IV beta 3; neuregulin 1; Neuregulin1; Neuregulin-1; NRG1; pro-neuregulin-1, membrane-bound isoform; pro-NRG1; sensory and motor neuron derived factor; SMDF

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Citations for Human Neuregulin-1/NRG1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

18 Citations: Showing 1 - 10
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  1. Real-time imaging of mitochondrial redox reveals increased mitochondrial oxidative stress associated with amyloid ? aggregates in vivo in a mouse model of Alzheimer's disease
    Authors: Calvo-Rodriguez, M;Kharitonova, EK;Snyder, AC;Hou, SS;Sanchez-Mico, MV;Das, S;Fan, Z;Shirani, H;Nilsson, KPR;Serrano-Pozo, A;Bacskai, BJ;
    Molecular neurodegeneration
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  2. Rab7a activation promotes degradation of select tight junction proteins at the blood-brain barrier after ischemic stroke
    Authors: Cottarelli A, Shahriar S, Arac A et al.
    bioRxiv
  3. Targeting the TCA cycle can ameliorate widespread axonal energy deficiency in neuroinflammatory lesions
    Authors: Tai YH, Engels D, Locatelli G et al.
    Nature Metabolism
  4. Adding of neurotensin to non-small cell lung cancer cells increases tyrosine phosphorylation of HER3
    Authors: TW Moody, I Ramos-Alva, RT Jensen
    Peptides, 2022-08-03;156(0):170858.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  5. Mu and Delta Opioid Receptor Targeting Reduces Connexin 43-Based Heterocellular Coupling during Neuropathic Pain
    Authors: Vicario N, Denaro S, Turnaturi R et al.
    International Journal of Molecular Sciences
  6. Chemogenetic stimulation of the G(i) pathway in astrocytes suppresses neuroinflammation
    Authors: Kim JH, Rahman MH, Lee WH, Suk K.
    Pharmacology Research & Perspectives
  7. Epigenetic treatment of behavioral and physiological deficits in a tauopathy mouse model
    Authors: Wang W, Cao Q, Tan T et al.
    Aging Cell
  8. An N-terminal fusion allele to study melanin concentrating hormone receptor 1
    Authors: Jasso KR, Kamba TK, Zimmerman AD et al.
    genesis
  9. mTOR kinase inhibition disrupts neuregulin 1-ERBB3 autocrine signaling and sensitizes NF2-deficient meningioma cellular models to IGF1R inhibition
    Authors: RL Beauchamp, S Erdin, L Witt, JT Jordan, SR Plotkin, JF Gusella, V Ramesh
    The Journal of Biological Chemistry, 2020-12-09;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. Soluble TREM2 ameliorates pathological phenotypes by modulating microglial functions in an Alzheimer's disease model
    Authors: L Zhong, Y Xu, R Zhuo, T Wang, K Wang, R Huang, D Wang, Y Gao, Y Zhu, X Sheng, K Chen, N Wang, L Zhu, D Can, Y Marten, M Shinohara, CC Liu, D Du, H Sun, L Wen, H Xu, G Bu, XF Chen
    Nat Commun, 2019-03-25;10(1):1365.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  11. miR-296-5p suppresses EMT of hepatocellular carcinoma via attenuating NRG1/ERBB2/ERBB3 signaling
    Authors: DM Shi, LX Li, XY Bian, XJ Shi, LL Lu, HX Zhou, TJ Pan, J Zhou, J Fan, WZ Wu
    J. Exp. Clin. Cancer Res., 2018-11-29;37(1):294.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  12. Expression of the neuroprotective protein aryl hydrocarbon receptor nuclear translocator 2 correlates with neuronal stress and disability in models of multiple sclerosis
    Authors: T Rahim, P Becquart, ME Baeva, J Quandt
    J Neuroinflammation, 2018-09-19;15(1):270.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC
  13. BRD4 amplification facilitates an oncogenic gene expression program in high-grade serous ovarian cancer and confers sensitivity to BET inhibitors
    Authors: GW Rhyasen, Y Yao, J Zhang, A Dulak, L Castriotta, K Jacques, W Zhao, F Gharahdagh, MM Hattersley, PD Lyne, E Clark, M Zinda, SE Fawell, GB Mills, H Chen
    PLoS ONE, 2018-07-23;13(7):e0200826.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  14. Dual effects of carbon monoxide on pericytes and neurogenesis in traumatic brain injury
    Nat Med, 2016-09-26;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC, Western Blot
  15. Fibroblast-derived neuregulin 1 promotes compensatory ErbB3 receptor signaling in mutant BRAF melanoma.
    Authors: Capparelli C, Rosenbaum S, Berger A, Aplin A
    J Biol Chem, 2015-08-12;290(40):24267-77.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  16. BRCA1-IRIS inactivation sensitizes ovarian tumors to cisplatin.
    Authors: Paul B, Blanchard Z, Ridgway M, ElShamy W
    Oncogene, 2014-08-18;34(23):3036-52.
    Species: Human
    Sample Types: Cell Culture Supernates, Whole Cells
    Applications: ELISA Development (Capture), Neutralization
  17. The NRG1 gene is frequently silenced by methylation in breast cancers and is a strong candidate for the 8p tumour suppressor gene.
    Authors: Chua YL, Ito Y, Pole JC, Newman S, Chin SF, Stein RC, Ellis IO, Caldas C, O'Hare MJ, Murrell A, Edwards PA
    Oncogene, 2009-10-05;28(46):4041-52.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  18. Fractalkine/CX3CL1 enhances GABA synaptic activity at serotonin neurons in the rat dorsal raphe nucleus.
    Authors: Heinisch S, Kirby LG
    Neuroscience, 2009-09-10;164(3):1210-23.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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