Human Neuropeptide Y/NPY Antibody

Catalog #: MAB85172 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB85172-100
MAB85172-SP

Human Neuropeptide Y/NPY ELISA Standard Curve.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Neuropeptide Y/NPY Antibody Summary

Species Reactivity

Human

Specificity

Detects Neuropeptide Y/NPY in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 904049

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Neuropeptide Y/NPY conjugated to KLH
Accession # P01303

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human Neuropeptide Y/NPY Monoclonal Antibody (Catalog # MAB85171).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Neuropeptide Y/NPY DuoSet ELISA Kit (Catalog # DY8517-05) for convenient development of a sandwich ELISA.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA

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Human Neuropeptide Y/NPY ELISA Standard Curve. Recombinant Human Neuropeptide Y/NPY protein was serially diluted 2-fold and captured by Mouse Anti-Human Neuropeptide Y/NPY Monoclonal Antibody (Catalog # MAB85171) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Neuropeptide Y/NPY Monoclonal Antibody (Catalog # MAB85172) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neuropeptide Y/NPY

Neuropeptide Y (NPY) is a 36 amino acid peptide that was isolated from hypothalamus in porcine brain in 1982 and lately it belongs to a family of peptides which include Pancreatic Polypeptide (PP) and Peptide YY (PYY) which exert their pharmacological action via interaction with G-protein coupled receptors Y1, Y2, Y4, Y5 and y6. NPY is the most abundant peptide in brain and in nervous system NPY functions as a neurotransmitter regulating many processes including memory and learning, pain, fat storage and blood pressure. NPY also regulates stress by stimulating secretion of corticotropin-releasing hormone in brain. It appears there is a correlation between the increased levels of NPY gene expression in hippocampus and epileptic seizures. Cocaine reduces the levels of NPY and such a decrease is thought to be related to depression and anxiety. NPY receptors are rhodopsin-like G-protein coupled receptors (GPCR) coupled to G_i or G₀ proteins, which inhibit adenylate cyclase and reduce cAMP accumulation and modulate Calcium and Potassium channels.

Entrez Gene IDs

4852 (Human); 109648 (Mouse); 24604 (Rat); 397304 (Porcine)

Alternate Names

170 kDa melanoma membrane-bound gelatinase; DKFZp686G13158; DPPIV; EC 3.4.21.-; FAPA; Fibroblast activation protein alpha; fibroblast activation protein, alpha; Integral membrane serine protease; Neuropeptide Y; NPY; PYY4; seprase