Human Neuroplastin Isoform 1 Antibody

Catalog #: AF5174 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5174
AF5174-SP

Detection of Human Neuroplastin by Western Blot.

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Human Neuroplastin Isoform 1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Neuroplastin in direct ELISAs and Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Neuroplastin 55 isoform 1
Gln29-Ser219
Accession # Q9Y639.1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.2 µg/mL

See below

Immunohistochemistry

1-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Neuroplastin by Western Blot. Western blot shows lysates of human brain (cerebellum, hypothalamus, and hippocampus) tissue. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human Neuroplastin Isoform 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5174) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Neuroplastin at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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Neuroplastin in Human Brain. Neuroplastin was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using 1.7 µg/mL Sheep Anti-Human Neuroplastin Isoform 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5174) overnight at 4 °C. Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific labeling was localized to the dendrites of Purkinje cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neuroplastin

Neuroplastin (NPTN; also stromal cell-derived receptor 1 and Np55) is a 52‑57 kDa member of the Ig-superfamily. It is widely expressed, and likely serves as a cell adhesion molecule in multiple tissues. Human Neuroplastin is 282 amino acids (aa) in length. It is a type I transmembrane glycoprotein that contains two Ig-like domains (aa 32‑119 and 122‑213) and a 38 aa cytoplasmic region (aa 245‑282). There is a brain-enriched 65 kDa form (Np65) that negatively regulates LTP in the hippocampus. It contains an N-terminal 116 aa Ig-like domain-containing insert after Asn30, and interacts homophilically. There is a third potential splice variant that shows an alternate start site at Met85 accompanied by a 23 aa substitution for aa 200‑282. Over aa 29‑229, human Neuroplastin is 91% aa identical to mouse Neuroplastin.

Entrez Gene IDs

27020 (Human); 20320 (Mouse); 56064 (Rat)

Alternate Names

AW554172; GP55; GP55Stromal cell-derived receptor 1; GP65SDR-1; GP66; MGC102805; Neuroplastin; np55; np65; NPTN; SDFR1; SDFR1DKFZp686L2477; SDR1; SDR1stromal cell derived factor receptor 1