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Human NGL-3/LRRC4B Antibody

Catalog #: MAB69191
1 Citation Datasheet / COA / SDS
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MAB69191
MAB69191-SP
Detection of Human NGL‑3/LRRC4B by Western Blot.
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Human NGL-3/LRRC4B Antibody Summary

Species Reactivity
Human
Specificity
Detects human NGL-3/LRRC4B in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) LRRC3, rhLRRC4, or rhLRRC32 is observed.
Source
Monoclonal Mouse IgG2A Clone # 702008
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human NGL-3/LRRC4B
Ala47-Lys576
Accession # Q9NT99
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Purity
Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human NGL-3/LRRC4B antibody by Western Blot. View Larger

Detection of Human NGL‑3/LRRC4B by Western Blot. Western blot shows lysates of human brain (hypothalamus) tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human NGL-3/LRRC4B Monoclonal Antibody (Catalog # MAB69191) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for NGL-3/LRRC4B at approximately 115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NGL-3/LRRC4B

NGL-3 (netrin-G ligand-3), also called LRRC4B (Leu-rich repeat/LRR-containing glycoprotein 4B), is a 72 kDa (predicted) post-synaptic adhesion molecule of the LRR protein family. Mature human LRRC4B is a 678 amino acid (aa) type I transmembrane protein with a 541 aa extracellular region (aa 36-576) containing nine LRRs, a C2-type Ig-like domain, and a Thr-rich segment. Over the region used as an immunogen, human LRRC4B shares 97% aa sequence identity with mouse and rat LRRC4B. LRRC4B also shares ~55% aa identity with LRRC4C/NGL-1 and LRRC4/NGL-2, but each recognizes different ligands. LRRC4B recognition of the receptor tyrosine phosphatases LAR/PTPRF, PTPR-d and PTPR-s induces excitatory synapse formation.

Long Name
Netrin-G3 Ligand/Leucine-rich Repeat Containing 4B
Entrez Gene IDs
94030 (Human); 272381 (Mouse); 308571 (Rat)
Alternate Names
DKFZp761A179; HSM; Leucine Rich Repeat Containing 4B; Leucine-Rich Repeat-Containing Protein 4B; Leucine-Rich Repeats And Immunoglobulin-Like Domains 4; LRIG4; LRRC4B; Netrin-G3 Ligand; NGL3; NGL-3

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Citation for Human NGL-3/LRRC4B Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Tandem Mass Tag LC-MS/MS of Aqueous Humor From Individuals With Type 2 Diabetes Without Retinopathy Reveals Early Dysregulation of Synaptic Proteins
    Authors: Sachdeva, MM;Lee, Y;Unlu, EK;Koseoglu, ND;Cha, E;Wang, J;Prescott, CR;Eghrari, AO;Na, CH;
    Investigative ophthalmology & visual science
    Species: Human hepegivirus
    Sample Types: Aqueous Humor
    Applications: Western Blot

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