Human Nidogen-1/Entactin Antibody Summary
Leu29-Lys1114 (Gln1113Arg)
Accession # AAH45606.1
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Applications
Human Nidogen-1/Entactin Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Nidogen‑1/Entactin in Human Heart. Nidogen‑1/Entactin was detected in immersion fixed paraffin-embedded sections of human heart using Mouse Anti-Human Nidogen‑1/Entactin Monoclonal Antibody (Catalog # MAB2570) at 5 µg/ml for 1 hour at room temperature followed by incubation with the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007) or the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Nidogen‑1/Entactin in Human Chondrocytes. Nidogen-1/Entactin was detected in immersion fixed human mesenchymal stem cells differentiated into chondrocytes using Mouse Anti-Human Nidogen-1/Entactin Monoclonal Antibody (Catalog # MAB2570) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Nidogen-1/Entactin
Nidogen-1 (also entactin) is a 150 kDa, secreted, monomeric glycoprotein that serves as a major linking component of basement membranes (1-4). It is synthesized as a 1247 amino acid (aa) precursor with a 28 aa signal sequence and a 1219 aa mature protein. The molecule is modular in structure with five distinct regions. There are three globular domains (G1-3) separated by a mucin region and an extended rod-shaped segment (5-7). The N-terminal globular domain (G1) is 200 aa in length and seemingly unrelated to any known motif (8). The mucin region is nearly 160 aa in length and presumably O-glycosylated (2, 8). G2 and G3 are both approximately 300 aa in length. G2 is described as a Nidogen ( beta -barrel) domain, while C-terminal G3 assumes a beta -propeller configuration (1). The 250 aa rod-shaped segment has multiple EGF-like motifs and two thyroglobulin type 1 domains. Functionally, G1 is reported to bind type IV collagen (2, 7). The mucin region contains a short peptide that ligates alpha 3 beta 1 integrins (9, 10). G2 interacts with perlecan, and an RGD motif in the rod-shaped segment serves as a binding site for alpha v beta 3 integrins (9, 10). Finally, G3 is associated with laminin binding (2, 7). As a full-length molecule, the multiple extracellular matrix-binding sites of Nidogen-1 are well positioned to serve as anchor sites for basement membrane molecules. Nidogen-1 also undergoes proteolytic processing by at least two MMPs, MMP-7 and MMP-19 (10, 11). While this destroys the integrity of Nidogen-associated matrices, it also generates peptide fragments that are capable of inducing neutrophil chemotaxis and phagocytosis (10). Nidogen-2 is related to Nidogen-1 (≈ 50% aa identity) and shares many of the same adhesive properties as Nidogen-1 (12). Both bind perlecan plus collagens I and IV. Nidogen‑2, however, does not bind fibulin-1 or 2, and shows only modest interaction with laminin. Thus, although coexpressed, Nidogen-2 serves as only a partial substitute for Nidogen-1 (2, 12). Human Nidogen-1 shares 85% aa sequence identity with both mouse and rat Nidogen-1, and 88% aa sequence identity with canine Nidogen-1.
- Hohenester, E. and J. Engel (2002) Matrix Biol. 21:115.
- Miosge, N. et al. (2001) Histochem. J. 33:523.
- Charonis, A. et al. (2005) Curr. Med. Chem. 12:1495.
- Timpl, R. and J.C. Brown (1996) BioEssays 18:123.
- Nagayoshi, T. et al. (1989) DNA 8:581.
- Zimmerman, K. et al. (1995) Genomics 27:245.
- Fox, J.W. et al. (1991) EMBO J. 10:3137.
- Mayer, U. et al. (1995) Eur. J. Biochem. 227:681.
- Gresham, H.D. et al. (1996) J. Biol. Chem. 271:30587.
- Dong, L-J. et al. (1995) J. Biol. Chem. 270:15383.
- Titz, B. et al. (2004) Cell. Mol. Life Sci. 61:1826.
- Kohfeldt, K. et al. (1998) J. Mol. Biol. 282:99.
Product Datasheets
Citations for Human Nidogen-1/Entactin Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Alterations of epithelial stem cell marker patterns in human diabetic corneas and effects of c-met gene therapy
Authors: Mehrnoosh Saghizadeh, Siavash Soleymani, Angel Harounian, Bhavik Bhakta, Sergey M. Troyanovsky, William J. Brunken et al.
Mol Vis
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Visualization of basement membranes by a nidogen-based fluorescent reporter in mice
Authors: Sugiko Futaki, Ayano Horimoto, Chisei Shimono, Naoko Norioka, Yukimasa Taniguchi, Hitomi Hamaoka et al.
Matrix Biology Plus
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Stromal-like Wilms tumor cells induce human Natural Killer cell degranulation and display immunomodulatory properties towards NK cells
Authors: Claudia Cantoni, Martina Serra, Erica Parisi, Bruno Azzarone, Angela Rita Sementa, Luigi Aurelio Nasto et al.
OncoImmunology
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Endothelial cell-derived nidogen-1 inhibits migration of SK-BR-3 breast cancer cells
Authors: DA Ferraro, F Patella, S Zanivan, C Donato, N Aceto, M Giannotta, E Dejana, M Diepenbruc, G Christofor, M Buess
BMC Cancer, 2019-04-04;19(1):312.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Enhanced wound healing, kinase and stem cell marker expression in diabetic organ-cultured human corneas upon MMP-10 and cathepsin F gene silencing.
Authors: Saghizadeh M, Epifantseva I, Hemmati D, Ghiam C, Brunken W, Ljubimov A
Invest Ophthalmol Vis Sci, 2013-12-17;54(13):8172-80.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Normalization of wound healing and diabetic markers in organ cultured human diabetic corneas by adenoviral delivery of c-Met gene.
Authors: Saghizadeh M, Kramerov AA, Yu FS, Castro MG, Ljubimov AV
Invest. Ophthalmol. Vis. Sci., 2009-11-20;51(4):1970-80.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Novel nanopolymer RNA therapeutics normalize human diabetic corneal wound healing and epithelial stem cells
Authors: Andrei A. Kramerov, Ruchi Shah, Hui Ding, Eggehard Holler, Sue Turjman, Yaron S. Rabinowitz et al.
Nanomedicine: Nanotechnology, Biology and Medicine
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Adenovirus-driven overexpression of proteinases in organ-cultured normal human corneas leads to diabetic-like changes.
Authors: Saghizadeh M, Kramerov AA, Yaghoobzadeh Y et al.
Brain Res Bull
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Normalization of wound healing and stem cell marker patterns in organ-cultured human diabetic corneas by gene therapy of limbal cells.
Authors: Saghizadeh M, Dib Cm, Brunken Wj et al.
Exp. Eye Res.
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Identification of NID1 as a novel candidate susceptibility gene for familial non-medullary thyroid carcinoma using whole-exome sequencing
Authors: Luis Eduardo Barbalho de Mello, Thaise Nayane Ribeiro Carneiro, Aline Neves Araujo, Camila Xavier Alves, Pedro Alexandre Favoretto Galante, Vanessa Candiotti Buzatto et al.
Endocrine Connections
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1% formalin fixed
Staining for basement membrane
Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma
