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Human NKG2D/CD314 Antibody

Catalog #: MAB139
100 Citations 2 Reviews Datasheet / COA / SDS
Clone 149810 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
MAB139-SP
MAB139-500
MAB139-100
Detection of NKG2D/CD314 in Human PBMCs gated on CD3- cells by Flow Cytometry.
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Citations (100)
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Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human NKG2D/CD314 Antibody Summary

Species Reactivity
Human
Specificity
Detects human NKG2D/CD314 in Western blots.
Source
Monoclonal Mouse IgG1 Clone # 149810
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
BaF3 mouse pro-B cell line transfected with human NKG2D/CD314
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human NKG2D/CD314 Fc Chimera (Catalog # 1299-NK)
under non-reducing conditions only
Flow Cytometry
0.25 µg/106 cells
See below
Costimulation of T Cells
Rincon-Orozco, B. et al. (2005) J. Immunol. 175:2144.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Neutralization
Kwong, K.Y. et al. (2008) J. Mol. Biol. 384:1143.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of NKG2D/CD314 antibody in Human PBMCs gated on CD3- cells antibody by Flow Cytometry. View Larger

Detection of NKG2D/CD314 in Human PBMCs gated on CD3- cells by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) gated on CD3- cells were stained with Mouse Anti-Human NCAM-1/CD56 APC-conjugated Monoclonal Antibody (Catalog # FAB2408A) and either (A) Mouse Anti-Human NKG2D/CD314 Monoclonal Antibody (Catalog # MAB139) or (B) Mouse IgG1 Isotype Control (Catalog # MAB002) followed by anti-Mouse IgG PE-conjugated Monoclonal Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry View Larger

Detection of NKG2D/CD314 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD8 APC-conjugated Monoclonal Antibody (FAB1509A) and either (A) Mouse Anti-Human NKG2D/CD314 Monoclonal Antibody (Catalog # MAB139) or (B) Mouse IgG1 Isotype Control (MAB002) followed by anti-Mouse IgG PE-conjugated Monoclonal Antibody (F0102B). Staining was performed using our Staining Membrane-associated Proteins protocol.

Flow Cytometry Detection of Human NKG2D/CD314 by Flow Cytometry View Larger

Detection of Human NKG2D/CD314 by Flow Cytometry Multiple receptors and ligands are involved in NK cell-mediated lysis of activated CD4+ T cells.Role of (A) activating and (B) inhibitory NK receptors in NK cell degranulation. Left column: representative histograms (of n≥3) for surface expression of ligands on activated (thick black line) and resting CD4+ T cells (thin black line). Isotype-matched control Ig are represented by dashed line (activated CD4+ T) and filled histogram (resting CD4+ T). Middle- and right column: NK and CD4+ T cells were activated for 4 days in vitro as described, and co-cultured for 4 hours with 10 ug/mL mAb (or relevant isotype-matched control Ig). Degranulation is shown for CD56dim (middle column) and CD56bright (right column) NK cells. Representative histograms of surface expression of receptors on activated (thick black line) and resting NK cells (thin black line). Isotype-matched control Ig are represented by dashed line (activated NK) and filled histogram (resting NK). * P<0.05, ** P<0.005, *** P<0.001. (C) Sorted IL-2-activated CD56dim and CD56bright NK cells were co-cultured with 51Cr-labeled activated CD4+ T cells in a 51Cr-release assay with human IgG4 isotype control (•) or anti-NKG2A mAb (○). Data represents n = 3 experiments. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/22384114), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NKG2D/CD314

NKG2D, also known as CD314, is a type II transmembrane glycoprotein having an extracellular lectin-like domain. This domain lacks the recognizable calcium-binding sites found in true C‑type lectins and binds protein rather than carbohydrate ligands. Human NKG2D is expressed on CD8+ alpha beta T cells, gamma δ T cells, NK cells, and NKT cells. In mouse systems NKG2D also occurs on macrophages. Human ligands for NKG2D include MICA, MICB, and ULBP1, 2, and 3. Expression of NKG2D ligands occurs in epithelial cells, tumor cells and under conditions of stress or infection. NKG2D exists as a disulfide-linked homodimer that delivers an activating signal upon ligand binding. Signaling requires association with an adapter protein. Alternative splicing of the NKG2D mRNA results in isoforms with different cytoplasmic domains that can associate either with DAP12 to deliver a true activating signal or with DAP10 resulting in a costimulatory signal. NKG2D has been implicated in anti-tumor surveillance and the immune response against viral infection.

References
  1. Li, P. et al. (2001) Nature Immunol. 2:443.
  2. Steinle, A. et al. (2001) Immunogenetics 53:279.
  3. Cosman, D. et al. (2001) Immunity 14:123.
  4. Cerwenka, A. and L. Lanier (2001) Immunol. Rev. 181:158.
  5. Wu, J. et al. (1999) Science 285:730.
  6. Diefenbach, A. et al. (2002) Nature Immunol. 3:1142.
  7. Gilfillan, S. et al. (2002) Nature Immunol. 3:1150.
  8. Groh, V. et al. (2001) Nature Immunol. 2:255.
  9. Cerwenka, A. et al. (2001) Proc. Natl. Acad. Sci. USA 98:11521.
  10. Diefenbach, A. et al. (2001) Nature 413:165.
  11. NKG2D and its Ligands (2002) www.RnDSystems.com.
Long Name
Natural Killer G2D
Entrez Gene IDs
22914 (Human); 27007 (Mouse); 102120479 (Cynomolgus Monkey)
Alternate Names
CD314 antigen; CD314; D12S2489E; FLJ17759; FLJ75772; Killer cell lectin-like receptor subfamily K member 1; killer cell lectin-like receptor subfamily K, member 1; KLR; KLRK1; NK cell receptor D; NKG2-D type II integral membrane protein; NKG2D; NKG2-D; NKG2-D-activating NK receptor; NKG2DDNA segment on chromosome 12 (unique) 2489 expressed sequence

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Citations for Human NKG2D/CD314 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

100 Citations: Showing 1 - 10
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  1. Low‑dose valproic acid with low‑dose gemcitabine augments MHC class I‑related chain A/B expression without inducing the release of soluble MHC class I‑related chain A/B
    Authors: Tomoharu Miyashita, Kenji Miki, Takashi Kamigaki, Isamu Makino, Hidehiro Tajima, Shinichi Nakanuma et al.
    Oncology Letters
  2. Multiplex interrogation of the NK cell signalome reveals global downregulation of CD16 signaling during lentivirus infection through an IL-18/ADAM17-dependent mechanism
    Authors: Sugawara S, Hueber B, Woolley G et al.
    PLoS pathogens
  3. NKG2D ligand expression in Crohn's disease and NKG2D-dependent stimulation of CD8+ T cell migration
    Authors: Kasper Vadstrup, Elisabeth Douglas Galsgaard, Helle Jensen, Lewis L. Lanier, James C. Ryan, Shih-Yu Chen et al.
    Experimental and Molecular Pathology
  4. Inhibition of the B7-H3 immune checkpoint limits tumor growth by enhancing cytotoxic lymphocyte function
    Authors: Young-hee Lee, Natalia Martin-Orozco, Peilin Zheng, Jing Li, Peng Zhang, Haidong Tan et al.
    Cell Research
  5. CD28 Homolog Is a Strong Activator of Natural Killer Cells for Lysis of B7H7+ Tumor Cells
    Authors: Xiaoxuan Zhuang, Eric O. Long
    Cancer Immunology Research
  6. ADAM10 new selective inhibitors reduce NKG2D ligand release sensitizing Hodgkin lymphoma cells to NKG2D-mediated killing
    Authors: Maria Raffaella Zocchi, Caterina Camodeca, Elisa Nuti, Armando Rossello, Roberta Venè, Francesca Tosetti et al.
    OncoImmunology
  7. NKG2D- and T-cell receptor-dependent lysis of malignant glioma cell lines by human gamma δ T cells: Modulation by temozolomide and A disintegrin and metalloproteases 10 and 17 inhibitors
    Authors: Guranda Chitadze, Marcus Lettau, Stefanie Luecke, Ting Wang, Ottmar Janssen, Daniel Fürst et al.
    OncoImmunology
  8. CD16 pre-ligation by defucosylated tumor-targeting mAb sensitizes human NK cells to gamma c cytokine stimulation via PI3K/mTOR axis
    Authors: Cristina Capuano, Chiara Pighi, Roberta Maggio, Simone Battella, Stefania Morrone, Gabriella Palmieri et al.
    Cancer Immunology, Immunotherapy
  9. Downregulation of the stress-induced ligand ULBP1 following SV40 infection confers viral evasion from NK cell cytotoxicity
    Authors: Yoav Bauman, Nir Drayman, Orly Ben-Nun-Shaul, Alon Vitenstein, Rachel Yamin, Yael Ophir et al.
    Oncotarget
  10. A bispecific ?? T-cell engager targeting EGFR activates a potent V?9V?2 T cell-mediated immune response against EGFR-expressing tumors
    Authors: King, LA;Toffoli, EC;Veth, M;Iglesias-Guimarais, V;Slot, MC;Amsen, D;van de Ven, R;Derks, S;Fransen, MF;Tuynman, JB;Riedl, T;Roovers, RC;Adang, AEP;Ruben, JM;Parren, PWHI;de Gruijl, TD;van der Vliet, HJ;
    Cancer immunology research
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  11. Involvement of ILC1-like innate lymphocytes in human autoimmunity, lessons from alopecia areata
    Authors: R Laufer Bri, A Keren, M Bertolini, Y Ullmann, R Paus, A Gilhar
    Elife, 2023-03-17;12(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  12. Specific alterations in NKG2D+ T lymphocytes in relapsing-remitting and progressive multiple sclerosis patients
    Authors: A Carmena Mo, Y Carpentier, F Lemaître, N Farzam-Kia, S Da Cal, JV Guimond, E Haddad, P Duquette, JM Girard, A Prat, C Larochelle, N Arbour
    Multiple sclerosis and related disorders, 2023-01-26;71(0):104542.
    Species: Human
    Sample Types: Whole Cells
    Applications: Blocking/Neutralizing
  13. Immunophenotype and antitumor activity of cytokine-induced killer cells from patients with hepatocellular carcinoma
    Authors: CK Yang, CH Huang, CH Hu, JH Fang, TC Chen, YC Lin, CY Lin
    PLoS ONE, 2023-01-04;18(1):e0280023.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  14. Engineering of immune checkpoints B7-H3 and CD155 enhances immune compatibility of MHC-I-/- iPSCs for beta cell replacement
    Authors: R Chimienti, T Baccega, S Torchio, F Manenti, S Pellegrini, A Cospito, A Amabile, MT Lombardo, P Monti, V Sordi, A Lombardo, M Malnati, L Piemonti
    Cell Reports, 2022-09-27;40(13):111423.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  15. Immunomodulatory Effects of IFNalpha on T and NK Cells in Chronic Myeloid Leukemia Patients in Deep Molecular Response Preparing for Treatment Discontinuation
    Authors: MC Puzzolo, M Breccia, P Mariglia, G Colafigli, S Pepe, E Scalzulli, E Mariggiò, R Latagliata, A Guarini, R Foà
    Journal of Clinical Medicine, 2022-09-23;11(19):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. The metabolism of cells regulates their sensitivity to NK cells depending on p53 status
    Authors: S Belkahla, JM Brualla, A Fayd'herbe, P Falvo, N Allende-Ve, M Constantin, AUH Khan, L Coenon, C Alexia, G Mitola, P Massa, S Orecchioni, F Bertolini, W Mnif, J Hernandez, A Anel, M Villalba
    Scientific Reports, 2022-02-25;12(1):3234.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  17. Metformin sensitizes leukemic cells to cytotoxic lymphocytes by increasing expression of intercellular adhesion molecule-1 (ICAM-1)
    Authors: N Allende-Ve, J Marco Brua, P Falvo, C Alexia, M Constantin, AF de Maudave, L Coenon, D Gitenay, G Mitola, P Massa, S Orecchioni, F Bertolini, I Marzo, A Anel, M Villalba
    Scientific Reports, 2022-01-25;12(1):1341.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  18. Venetoclax enhances NK cell killing sensitivity of AML cells through the NKG2D/NKG2DL activation pathway
    Authors: HY Wu, KX Li, WY Pan, MQ Guo, DZ Qiu, YJ He, YH Li, YX Huang
    International immunopharmacology, 2022-01-06;104(0):108497.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  19. The Epstein-Barr Virus Oncogene EBNA1 Suppresses Natural Killer Cell Responses and Apoptosis Early after Infection of Peripheral B Cells
    Authors: D Westhoff S, A Chakravort, M Hayes, W Hammerschm, B Sugden
    MBio, 2021-11-16;0(0):e0224321.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  20. Immunomodulatory effect of NEDD8-activating enzyme inhibition in Multiple Myeloma: upregulation of NKG2D ligands and sensitization to Natural Killer cell recognition
    Authors: S Petillo, C Capuano, R Molfetta, C Fionda, A Mekhloufi, C Pighi, F Antonangel, A Zingoni, A Soriani, MT Petrucci, R Galandrini, R Paolini, A Santoni, M Cippitelli
    Cell Death & Disease, 2021-09-04;12(9):836.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  21. The diterpenoid adenanthin upregulates the expression of natural killer group 2D receptor ligands in hepatocellular carcinoma cells
    Authors: X Zhong, H Liao, S Hu, K Luo, H Zhu
    Molecular and cellular probes, 2021-07-12;59(0):101759.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  22. The human cytomegalovirus protein UL147A downregulates the most prevalent MICA allele: MICA*008, to evade NK cell-mediated killing
    Authors: E Seidel, L Dassa, C Schuler, E Oiknine-Dj, DG Wolf, VTK Le-Trillin, O Mandelboim
    PloS Pathogens, 2021-05-03;17(5):e1008807.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  23. Human Metapneumovirus Escapes NK Cell Recognition through the Downregulation of Stress-Induced Ligands for NKG2D
    Authors: M Diab, D Schmiedel, E Seidel, E Bacharach, O Mandelboim
    Viruses, 2020-07-20;12(7):.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Neutralization
  24. Preclinical immunotherapy with Cytokine-Induced Killer lymphocytes against epithelial ovarian cancer
    Authors: S Capellero, J Erriquez, C Melano, G Mesiano, S Genta, A Pisacane, G Mittica, E Ghisoni, M Olivero, MF Di Renzo, M Aglietta, D Sangiolo, G Valabrega
    Sci Rep, 2020-04-15;10(1):6478.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  25. NKG2D/DAP10 Signaling recruits EVL to the cytotoxic synapse to generate F-actin and promote NK cell cytotoxicity
    Authors: KM Wilton, BL Overlee, DD Billadeau
    J. Cell. Sci., 2019-07-18;0(0):.
    Species: Human, Mouse
    Sample Types: Whole Cells
    Applications: Cytotoxicity Assay, Stimulation
  26. Pretreatment of Glioblastoma with Bortezomib Potentiates Natural Killer Cell Cytotoxicity through TRAIL/DR5 Mediated Apoptosis and Prolongs Animal Survival
    Authors: A Gras Navar, H Espedal, JV Joseph, L Trachsel-M, M Bahador, B Tore Gjert, E Klæboe Kri, A Simonsen, H Miletic, P Øyvind Eng, MA Rahman, M Chekenya
    Cancers (Basel), 2019-07-17;11(7):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  27. Arming oHSV with ULBP3 drives abscopal immunity in lymphocyte-depleted glioblastoma
    Authors: HG Wirsching, H Zhang, F Szulzewsky, S Arora, P Grandi, PJ Cimino, N Amankulor, JS Campbell, L McFerrin, SS Pattwell, C Ene, A Hicks, M Ball, J Yan, J Zhang, D Kumasaka, RH Pierce, M Weller, M Finer, C Quéva, JC Glorioso, AM Houghton, EC Holland
    JCI Insight, 2019-07-11;4(13):.
    Species: Human, Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  28. Cryopreserved Human Natural Killer Cells Exhibit Potent Antitumor Efficacy against Orthotopic Pancreatic Cancer through Efficient Tumor-Homing and Cytolytic Ability (Running Title: Cryopreserved NK Cells Exhibit Antitumor Effect)
    Authors: E Oh, B Min, Y Li, C Lian, J Hong, GM Park, B Yang, SY Cho, YK Hwang, CO Yun
    Cancers (Basel), 2019-07-09;11(7):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  29. Targetable mechanisms driving immunoevasion of persistent senescent cells link chemotherapy-resistant cancer to aging
    Authors: DP Muñoz, SM Yannone, A Daemen, Y Sun, F Vakar-Lope, M Kawahara, AM Freund, F Rodier, JD Wu, PY Desprez, DH Raulet, PS Nelson, LJ van 't Vee, J Campisi, JP Coppé
    JCI Insight, 2019-06-11;5(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  30. The Role of NK Cells in the Control of Viral Infection in HTLV-1 Carriers
    Authors: CF Amorim, NB Carvalho, JA Neto, SB Santos, MFR Grassi, LP Carvalho, EM Carvalho
    J Immunol Res, 2019-02-28;2019(0):6574828.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  31. CD56-negative NK cells with impaired effector function expand in CMV and EBV co-infected healthy donors with age
    Authors: B Müller-Dur, J Grählert, OP Devine, AN Akbar, C Hess
    Aging (Albany NY), 2019-01-27;11(2):724-740.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  32. Incomplete downregulation of CD4 expression affects HIV-1 Env conformation and ADCC responses
    Authors: J Prévost, J Richard, H Medjahed, A Alexander, J Jones, JC Kappes, C Ochsenbaue, A Finzi
    J. Virol., 2018-06-13;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  33. The combination of ionizing radiation and proteasomal inhibition by bortezomib enhances the expression of NKG2D ligands in multiple myeloma cells
    Authors: YS Lee, W Heo, J Nam, YH Jeung, J Bae
    J. Radiat. Res., 2018-05-01;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  34. EBV transformation induces overexpression of hMSH2/3/6 on B lymphocytes and enhances ??T cell-mediated cytotoxicity via TCR and NKG2D
    Authors: YM Dai, HY Liu, YF Liu, Y Zhang, W He
    Immunology, 2018-03-07;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  35. Actin retrograde flow controls natural killer cell response by regulating the conformation state of SHP-1
    Authors: O Matalon, A Ben-Shmuel, J Kivelevitz, B Sabag, S Fried, N Joseph, E Noy, G Biber, M Barda-Saad
    EMBO J., 2018-02-15;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  36. Impaired NK cell recognition of vemurafenib-treated melanoma cells is overcome by simultaneous application of histone deacetylase inhibitors
    Authors: S López-Cobo, N Pieper, C Campos-Sil, EM García-Cue, HT Reyburn, A Paschen, M Valés-Góme
    Oncoimmunology, 2017-11-06;7(2):e1392426.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  37. Human cytomegalovirus escapes immune recognition by NK cells through the downregulation of B7-H6 by the viral genes US18 and US20
    Authors: Y Charpak-Am, T Kubsch, E Seidel, E Oiknine-Dj, N Cavaletto, R Yamin, D Schmiedel, D Wolf, G Gribaudo, M Messerle, L Cicin-Sain, O Mandelboim
    Sci Rep, 2017-08-17;7(1):8661.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  38. Chromosome Mis-segregation Generates Cell-Cycle-Arrested Cells with Complex Karyotypes that Are Eliminated by the Immune System
    Authors: S Santaguida, A Richardson, DR Iyer, O M'Saad, L Zasadil, KA Knouse, YL Wong, N Rhind, A Desai, A Amon
    Dev. Cell, 2017-06-19;41(6):638-651.e5.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  39. Control of Tumor Initiation by NKG2D Naturally Expressed on Ovarian Cancer Cells
    Authors: X Cai, A Caballero-, MM Gewe, IC Jenkins, CW Drescher, RK Strong, T Spies, V Groh
    Neoplasia, 2017-05-09;19(6):471-482.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  40. Two alternate strategies for innate immunity to Epstein-Barr virus: One using NK cells and the other NK cells and ?? T cells
    Authors: Z Djaoud, LA Guethlein, A Horowitz, T Azzi, N Nemat-Gorg, D Olive, D Nadal, PJ Norman, C Münz, P Parham
    J. Exp. Med., 2017-05-03;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  41. Prognostic significance and functional implication of immune activating receptor NKG2D in gastric cancer
    Authors: F Lin, C Dai, X Ge, W Tang, Y Lin, Y Wang, J Li
    Biochem. Biophys. Res. Commun., 2017-04-20;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  42. EBI3 regulates the NK cell response to mouse cytomegalovirus infection
    Authors: H Jensen, SY Chen, L Folkersen, GP Nolan, LL Lanier
    Proc. Natl. Acad. Sci. U.S.A, 2017-01-31;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  43. Low-dose bortezomib increases the expression of NKG2D and DNAM-1 ligands and enhances induced NK and ?? T cell-mediated lysis in multiple myeloma
    Authors: C Niu, H Jin, M Li, S Zhu, L Zhou, F Jin, Y Zhou, D Xu, J Xu, L Zhao, S Hao, W Li, J Cui
    Oncotarget, 2017-01-24;8(4):5954-5964.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  44. Interleukin-15 stimulates natural killer cell-mediated killing of both human pancreatic cancer and stellate cells
    Authors: JRM Van Audena, J De Waele, E Marcq, J Van Loenho, E Lion, JMJ Van den Be, R Jesenofsky, A Masamune, G Roeyen, P Pauwels, F Lardon, M Peeters, ELJ Smits
    Oncotarget, 2017-01-01;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  45. A functional polymorphism in the NKG2D gene modulates NK-cell cytotoxicity and is associated with susceptibility to Human Papilloma Virus-related cancers
    Sci Rep, 2016-12-20;6(0):39231.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  46. SEP enhanced the antitumor activity of 5-fluorouracil by up-regulating NKG2D/MICA and reversed immune suppression via inhibiting ROS and caspase-3 in mice
    Authors: M Ke, H Wang, Y Zhou, J Li, Y Liu, M Zhang, J Dou, T Xi, B Shen, C Zhou
    Oncotarget, 2016-08-02;7(31):49509-49526.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  47. CD155 on HIV-Infected Cells Is Not Modulated by HIV-1 Vpu and Nef but Synergizes with NKG2D Ligands to Trigger NK Cell Lysis of Autologous Primary HIV-Infected Cells.
    Authors: Davis Z, Sowrirajan B, Cogswell A, Ward J, Planelles V, Barker E
    AIDS Res Hum Retroviruses, 2016-07-20;33(2):93-100.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  48. Human Induced Pluripotent Stem Cells Are Targets for Allogeneic and Autologous Natural Killer (NK) Cells and Killing Is Partly Mediated by the Activating NK Receptor DNAM-1.
    Authors: Kruse V, Hamann C, Monecke S, Cyganek L, Elsner L, Hubscher D, Walter L, Streckfuss-Bomeke K, Guan K, Dressel R
    PLoS ONE, 2015-05-07;10(5):e0125544.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  49. Cytotoxicity and infiltration of human NK cells in in vivo-like tumor spheroids.
    Authors: Giannattasio A, Weil S, Kloess S, Ansari N, Stelzer E, Cerwenka A, Steinle A, Koehl U, Koch J
    BMC Cancer, 2015-05-03;15(0):351.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  50. Central nervous system acute lymphoblastic leukemia: role of natural killer cells.
    Authors: Frishman-Levy L, Shemesh A, Bar-Sinai A, Ma C, Ni Z, Frenkel S, Muench V, Bruckmueller H, Vokuhl C, Debatin K, Eckert C, Stanulla M, Schrappe M, Campbell K, Loewenthal R, Schewe D, Hochman J, Meyer L, Kaufman D, Cario G, Porgador A, Izraeli S
    Blood, 2015-04-20;125(22):3420-31.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  51. A truncated human NKG2D splice isoform negatively regulates NKG2D-mediated function.
    Authors: Karimi M, Aguilar O, Zou B, Bachmann M, Carlyle J, Baldwin C, Kambayashi T
    J Immunol, 2014-08-04;193(6):2764-71.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  52. Dok1 and Dok2 proteins regulate natural killer cell development and function.
    Authors: Celis-Gutierrez J, Boyron M, Walzer T, Pandolfi P, Jonjic S, Olive D, Dalod M, Vivier E, Nunes J
    EMBO J, 2014-06-24;33(17):1928-40.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  53. c-Cbl regulates MICA- but not ULBP2-induced NKG2D down-modulation in human NK cells.
    Authors: Molfetta R, Quatrini L, Capuano C, Gasparrini F, Zitti B, Zingoni A, Galandrini R, Santoni A, Paolini R
    Eur J Immunol, 2014-06-17;44(9):2761-70.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  54. L-plastin is involved in NKG2D recruitment into lipid rafts and NKG2D-mediated NK cell migration.
    Authors: Serrano-Pertierra E, Cernuda-Morollon E, Brdicka T, Hooejsi V, Lopez-Larrea C
    J Leukoc Biol, 2014-05-06;96(3):437-45.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  55. Natural killer cells induce eosinophil activation and apoptosis.
    Authors: Awad A, Yassine H, Barrier M, Vorng H, Marquillies P, Tsicopoulos A, Duez C
    PLoS ONE, 2014-04-11;9(4):e94492.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  56. Cytokine-induced killer cells eradicate bone and soft-tissue sarcomas.
    Authors: Sangiolo, Dario, Mesiano, Giulia, Gammaitoni, Loretta, Leuci, Valeria, Todorovic, Maja, Giraudo, Lidia, Cammarata, Cristina, Dell'Aglio, Carmine, D'Ambrosio, Lorenzo, Pisacane, Alberto, Sarotto, Ivana, Miano, Sara, Ferrero, Ivana, Carnevale-Schianca, Fabrizio, Pignochino, Ymera, Sassi, Francesc, Bertotti, Andrea, Piacibello, Wanda, Fagioli, Franca, Aglietta, Massimo, Grignani, Giovanni
    Cancer Res, 2013-12-19;74(1):119-29.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  57. A unique secreted adenovirus E3 protein binds to the leukocyte common antigen CD45 and modulates leukocyte functions.
    Authors: Windheim, Mark, Southcombe, Jennifer, Kremmer, Elisabet, Chaplin, Lucy, Urlaub, Doris, Falk, Christin, Claus, Maren, Mihm, Janine, Braithwaite, Myles, Dennehy, Kevin, Renz, Harald, Sester, Martina, Watzl, Carsten, Burgert, Hans-Ger
    Proc Natl Acad Sci U S A, 2013-11-11;110(50):E4884-93.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  58. Modulation of NKG2D ligand expression and metastasis in tumors by spironolactone via RXRgamma activation.
    Authors: Leung W, Vong Q, Lin W, Janke L, Chen T, Leung W
    J Exp Med, 2013-11-04;210(12):2675-92.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  59. The road to memory: an early rest for the long journey.
    Authors: Ho, Liam Poc, Yit, Pui San, Ng, Lee Hui, Linn, Yeh Chin, Zhao, Yi, Sun, Li, Ling, Khoon Li, Chai Koh, Mickey B, Monica Shih, Meng-Chu, Li, Shang, Wang, Xue Ying, Tien, Sim Leng, Goh, Yeow Tee
    J Immunol, 2013-11-01;191(11):5603-14.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  60. CEACAM1 on activated NK cells inhibits NKG2D-mediated cytolytic function and signaling.
    Authors: Hosomi S, Chen Z, Baker K, Chen L, Huang Y, Olszak T, Zeissig S, Wang J, Mandelboim O, Beauchemin N, Lanier L, Blumberg R
    Eur J Immunol, 2013-06-18;43(9):2473-83.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  61. Ex vivo expansion of highly cytotoxic human NK cells by cocultivation with irradiated tumor cells for adoptive immunotherapy.
    Authors: Lim S, Kim T, Lee J, Sonn C, Kim K, Kim J, Choi J, Choi I, Yun C, Kim J, Yee C, Kumar V, Lee K
    Cancer Res, 2013-04-11;73(8):2598-607.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  62. Mechanisms of the antitumor activity of human Vgamma9Vdelta2 T cells in combination with zoledronic acid in a preclinical model of neuroblastoma.
    Authors: Di Carlo E, Bocca P, Emionite L, Cilli M, Cipollone G, Morandi F, Raffaghello L, Pistoia V, Prigione I
    Mol Ther, 2013-03-12;21(5):1034-43.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  63. Dedicator of cytokinesis 8 interacts with talin and Wiskott-Aldrich syndrome protein to regulate NK cell cytotoxicity.
    Authors: Ham, Hyoungju, Guerrier, Sabrice, Kim, JungJin, Schoon, Renee A, Anderson, Erik L, Hamann, Michael, Lou, Zhenkun, Billadeau, Daniel D
    J Immunol, 2013-03-01;190(7):3661-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  64. Analysis of Vdelta1 T cells in clinical grade melanoma-infiltrating lymphocytes.
    Authors: Donia M, Ellebaek E, Andersen M, Straten P, Svane I
    Oncoimmunology, 2012-11-01;1(8):1297-1304.
    Species: Human
    Sample Types: Whole Cells
    Applications: Blocking
  65. Super-resolution imaging of remodeled synaptic actin reveals different synergies between NK cell receptors and integrins.
    Authors: Brown A, Dobbie I, Alakoskela J, Davis I, Davis D
    Blood, 2012-09-10;120(18):3729-40.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  66. MiR-10b Downregulates the Stress-Induced Cell Surface Molecule MICB, a Critical Ligand for Cancer Cell Recognition by Natural Killer Cells.
    Authors: Tsukerman P, Stern-Ginossar N, Gur C, Glasner A, Nachmani D, Bauman Y, Yamin R, Vitenshtein A, Stanietsky N, Bar-Mag T, Lankry D, Mandelboim O
    Cancer Res, 2012-08-21;72(21):5463-72.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  67. Wiskott-Aldrich syndrome protein (WASp) and N-WASp are involved in the regulation of NK-cell migration upon NKG2D activation.
    Eur J Immunol, 2012-08-01;42(8):2142-51.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  68. Human TCR gamma delta+ T cells represent a novel target for IL-27 activity.
    Authors: Morandi F, Prigione I, Airoldi I
    Eur. J. Immunol., 2012-06-01;42(6):1547-52.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  69. NK cells from HCV-infected patients effectively induce apoptosis of activated primary human hepatic stellate cells in a TRAIL-, FasL- and NKG2D-dependent manner.
    Authors: Glassner A, Eisenhardt M, Kramer B, Korner C, Coenen M, Sauerbruch T, Spengler U, Nattermann J
    Lab. Invest., 2012-03-26;92(7):967-77.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  70. Cytotoxicity of CD56(bright) NK cells towards autologous activated CD4+ T cells is mediated through NKG2D, LFA-1 and TRAIL and dampened via CD94/NKG2A.
    Authors: Nielsen N, Odum N, Urso B, Lanier LL, Spee P
    PLoS ONE, 2012-02-22;7(2):e31959.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  71. 2-deoxy D-glucose prevents cell surface expression of NKG2D ligands through inhibition of N-linked glycosylation.
    Authors: Andresen L, Skovbakke SL, Persson G, Hagemann-Jensen M, Hansen KA, Jensen H, Skov S
    J. Immunol., 2012-01-06;188(4):1847-55.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  72. Tumor suppressive microRNAs miR-34a/c control cancer cell expression of ULBP2, a stress-induced ligand of the natural killer cell receptor NKG2D.
    Authors: Heinemann A, Zhao F, Pechlivanis S, Eberle J, Steinle A, Diederichs S, Schadendorf D, Paschen A
    Cancer Res., 2011-11-18;72(2):460-71.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  73. DNAM-1 ligand expression on Ag-stimulated T lymphocytes is mediated by ROS-dependent activation of DNA-damage response: relevance for NK-T cell interaction.
    Authors: Ardolino M, Zingoni A, Cerboni C, Cecere F, Soriani A, Iannitto ML, Santoni A
    Blood, 2011-03-15;117(18):4778-86.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, Neutralization
  74. Potent anti-tumor killing activity of the multifunctional Treg cell line HOZOT against human tumors with diverse origins.
    Authors: Inoue T, Tashiro Y, Takeuchi M
    Int. J. Oncol., 2011-03-03;38(5):1299-306.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  75. Natural killer cells from children with type 1 diabetes have defects in NKG2D-dependent function and signaling.
    Authors: Qin H, Lee IF, Panagiotopoulos C, Wang X, Chu AD, Utz PJ, Priatel JJ, Tan R
    Diabetes, 2011-01-26;60(3):857-66.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  76. NKG2A inhibits NKG2C effector functions of gammadelta T cells: implications in health and disease.
    Authors: Angelini DF, Zambello R, Galandrini R, Diamantini A, Placido R, Micucci F, Poccia F, Semenzato G, Borsellino G, Santoni A, Battistini L
    J. Leukoc. Biol., 2010-10-15;89(1):75-84.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  77. Analysis of HLDA9 mAbs on plasmacytoid dendritic cells.
    Authors: Cabezon R, Sintes J, Llinas L, Benitez-Ribas D
    Immunol. Lett., 2010-10-07;134(2):167-73.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  78. Cytotoxicity of activated natural killer cells against pediatric solid tumors.
    Authors: Cho D, Shook DR, Shimasaki N
    Clin. Cancer Res., 2010-06-11;16(15):3901-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  79. Human gammadelta T lymphocytes induce robust NK cell-mediated antitumor cytotoxicity through CD137 engagement.
    Authors: Maniar A, Zhang X, Lin W, Gastman BR, Pauza CD, Strome SE, Chapoval AI
    Blood, 2010-06-02;116(10):1726-33.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  80. SHP-2 expression negatively regulates NK cell function.
    Authors: Purdy AK, Campbell KS
    J. Immunol., 2009-11-13;183(11):7234-43.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  81. Natural killer lysis receptor (NKLR)/NKLR-ligand matching as a novel approach for enhancing anti-tumor activity of allogeneic NK cells.
    Authors: Markel G, Seidman R, Besser MJ
    PLoS ONE, 2009-05-19;4(5):e5597.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  82. Interferon-gamma down-regulates NKG2D ligand expression and impairs the NKG2D-mediated cytolysis of MHC class I-deficient melanoma by natural killer cells.
    Authors: Schwinn N, Vokhminova D, Sucker A, Textor S, Striegel S, Moll I, Nausch N, Tuettenberg J, Steinle A, Cerwenka A, Schadendorf D, Paschen A
    Int. J. Cancer, 2009-04-01;124(7):1594-604.
    Species: Human
    Sample Types: Whole Cells
    Applications: Blocking
  83. Detuning CD8+ T lymphocytes by down-regulation of the activating receptor NKG2D: role of NKG2D ligands released by activated T cells.
    Authors: Cerboni C, Ardolino M, Santoni A, Zingoni A
    Blood, 2009-01-05;113(13):2955-64.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  84. Generation, affinity maturation, and characterization of a human anti-human NKG2D monoclonal antibody with dual antagonistic and agonistic activity.
    Authors: Kwong KY, Baskar S, Zhang H, Mackall CL, Rader C
    J. Mol. Biol., 2008-09-16;384(5):1143-56.
    Species: Human
    Sample Types: Peptide, Whole Cells
    Applications: Neutralization, Surface Plasmon Resonance
  85. An unusual CD56(bright) CD16(low) NK cell subset dominates the early posttransplant period following HLA-matched hematopoietic stem cell transplantation.
    Authors: Dulphy N, Haas P, Busson M, Belhadj S, Peffault de Latour R, Robin M, Carmagnat M, Loiseau P, Tamouza R, Scieux C, Rabian C, Di Santo JP, Charron D, Janin A, Socie G, Toubert A
    J. Immunol., 2008-08-01;181(3):2227-37.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: Functional Assay, IHC-Fr
  86. NK cells recognize and lyse Ewing sarcoma cells through NKG2D and DNAM-1 receptor dependent pathways.
    Authors: Verhoeven DH, de Hooge AS, Mooiman EC, Santos SJ, ten Dam MM, Gelderblom H, Melief CJ, Hogendoorn PC, Egeler RM, van Tol MJ, Schilham MW, Lankester AC
    Mol. Immunol., 2008-07-26;45(15):3917-25.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  87. Induction of MHC class I-related chain B (MICB) by 5-aza-2'-deoxycytidine.
    Authors: Tang KF, He CX, Zeng GL, Wu J, Song GB, Shi YS, Zhang WG, Huang AL, Steinle A, Ren H
    Biochem. Biophys. Res. Commun., 2008-04-04;370(4):578-83.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  88. 4-1BB regulates NKG2D costimulation in human cord blood CD8+ T cells.
    Authors: Kim YJ, Han MK, Broxmeyer HE
    Blood, 2007-11-16;111(3):1378-86.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  89. Bortezomib down-regulates the cell-surface expression of HLA class I and enhances natural killer cell-mediated lysis of myeloma.
    Authors: Shi J, Tricot GJ, Garg TK, Malaviarachchi PA, Szmania SM, Kellum RE, Storrie B, Mulder A, Shaughnessy JD, Barlogie B, van Rhee F
    Blood, 2007-10-18;111(3):1309-17.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  90. 4-1BB is superior to CD28 costimulation for generating CD8+ cytotoxic lymphocytes for adoptive immunotherapy.
    Authors: Zhang H, Snyder KM, Suhoski MM, Maus MV, Kapoor V, June CH, Mackall CL
    J. Immunol., 2007-10-01;179(7):4910-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  91. Transfer of NKG2D and MICB at the cytotoxic NK cell immune synapse correlates with a reduction in NK cell cytotoxic function.
    Authors: Roda-Navarro P, Vales-Gomez M, Chisholm SE, Reyburn HT
    Proc. Natl. Acad. Sci. U.S.A., 2006-07-18;103(30):11258-63.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC, Neutralization
  92. Human plasmacytoid predendritic cells activate NK cells through glucocorticoid-induced tumor necrosis factor receptor-ligand (GITRL).
    Authors: Hanabuchi S, Watanabe N, Wang YH, Ito T, Shaw J, Cao W, Qin FX, Liu YJ
    Blood, 2006-01-05;107(9):3617-23.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Neutralization
  93. BCR/ABL promotes dendritic cell-mediated natural killer cell activation.
    Authors: Terme M, Borg C, Guilhot F, Masurier C, Flament C, Wagner EF, Caillat-Zucman S, Bernheim A, Turhan AG, Caignard A, Zitvogel L
    Cancer Res., 2005-07-15;65(14):6409-17.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  94. Receptors and lytic mediators regulating anti-tumor activity by the leukemic killer T cell line TALL-104.
    Authors: Brando C, Mukhopadhyay S, Kovacs E, Medina R, Patel P, Catina TL, Campbell KS, Santoli D
    J. Leukoc. Biol., 2005-06-03;78(2):359-71.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  95. High ERp5/ADAM10 expression in lymph node microenvironment and impaired NKG2D ligands recognition in Hodgkin lymphomas
    Authors: Maria Raffaella Zocchi, Silvia Catellani, Paolo Canevali, Sara Tavella, Anna Garuti, Barbara Villaggio et al.
    Blood
  96. Clearance of senescent decidual cells by uterine natural killer cells in cycling human endometrium
    Authors: Paul J Brighton, Yojiro Maruyama, Katherine Fishwick, Pavle Vrljicak, Shreeya Tewary, Risa Fujihara et al.
    eLife
  97. The septin cytoskeleton regulates natural killer cell lytic granule release
    Authors: Prasad V. Phatarpekar, Brittany L. Overlee, Alexander Leehan, Katelynn M. Wilton, Hyoungjun Ham, Daniel D. Billadeau
    Journal of Cell Biology
  98. A Trypanosoma brucei beta 3 glycosyltransferase superfamily gene encodes a beta 1-6 GlcNAc-transferase mediating N-glycan and GPI anchor modification
    Authors: Samuel M. Duncan, Rupa Nagar, Manuela Damerow, Dmitry V. Yashunsky, Benedetta Buzzi, Andrei V. Nikolaev et al.
    Journal of Biological Chemistry
  99. Blockade of N-Glycosylation Promotes Antitumor Immune Response of T Cells
    Authors: S Sasawatari, Y Okamoto, A Kumanogoh, T Toyofuku
    J. Immunol., 2020-01-22;0(0):.
  100. Sensitizing primary acute lymphoblastic leukemia to natural killer cell recognition by induction of NKG2D ligands
    Authors: Laura Jardine, Sophie Hambleton, Venetia Bigley, Sarah Pagan, Xiao-Nong Wang, Matthew Collin
    Leukemia & Lymphoma

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Human NKG2D/CD314 Antibody
By Anonymous on 01/24/2021
Application: Flow Sample Tested: Peripheral blood mononuclear cells (PBMCs) Species: Human
Flow Cytometry Human NKG2D/CD314 Antibody MAB139
Human NKG2D/CD314 Antibody
By Leslie Priddy on 04/13/2018
Application: IHC Sample Tested: Paraffined tumor tissue from HCC xenograft Species: Human