Human NKp30/NCR3 Antibody
Human NKp30/NCR3 Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Human NKp30 Antibody Induces IFN‑ gamma Secretion in NK‑92 Cells. Human NKp30 Monoclonal Antibody induces IFN-gamma secretion in the NK-92 human natural killer lymphoma cell line in a dose-dependent manner, as measured using the Human IFN-gamma DuoSet ELISA Kit (Catalog # DY285B). The ED50 for this effect is typically 0.2-1.2 µg/mL.
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Detection of NKp30/NCR3 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with either (A) Mouse Anti-Human NKp30/NCR3 Monoclonal Antibody (Catalog # MAB1849) or (B) Mouse IgG2A Isotype Control (MAB003) followed by anti-Mouse IgG APC-conjugated secondary antibody (F0101B) and Mouse Anti-Human NCAM-1/CD56 PE-conjugated Monoclonal Antibody (FAB2408P). Staining was performed using our Staining Membrane-associated Proteins protocol.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NKp30/NCR3
NKp30, along with NKp44 and NKp46, constitute a group of receptors termed “Natural Cytotoxicity Receptors” (1). These receptors play a major role in triggering NK‑mediated killing of most tumor cells lines. NKp30 is a type I transmembrane protein having a single extracellular V-like immunoglobulin domain (2). A physical association with the ITAM-bearing accessory protein, CD3 zeta, occurs via a charged residue in the NKp30 transmembrane domain. Ligation of NKp30 with a specific antibody results in phosphorylation of CD3 zeta (3). NKp30 is expressed on both resting and activated NK cells of the CD56dim, CD16+ subset that account for more that 85% of NK cells found in peripheral blood and spleen (4). NKp30 is absent from the CD56bright, CD16- subset that constitutes the majority of NK cells in lymph node and tonsil, however, its expression is up-regulated in these cells upon IL-2 activation (4). Studies with neutralizing antibodies reveal that NKp30 is partially responsible for triggering lytic activity against several tumor cell types and that it is the main receptor responsible for NK‑mediated lysis of immature dendritic cells (2, 5). The ligand(s) recognized by NKp30 has not been described.
- Moretta, L. and A. Moretta (2004) EMBO J. 23:255.
- Pende, D. et al. (1999) J. Exp. Med. 190:1505.
- Augugliaro, R. et al. (2003) Eur. J. Immunol. 33:1235.
- Ferlazzo, G. et al. (2004) J. Immunol. 172:1455.
- Ferlazzo, G. et al. (2002) J. Exp. Med. 195:343.
Product Datasheets
Citations for Human NKp30/NCR3 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 7
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Engineering of immune checkpoints B7-H3 and CD155 enhances immune compatibility of MHC-I-/- iPSCs for beta cell replacement
Authors: R Chimienti, T Baccega, S Torchio, F Manenti, S Pellegrini, A Cospito, A Amabile, MT Lombardo, P Monti, V Sordi, A Lombardo, M Malnati, L Piemonti
Cell Reports, 2022-09-27;40(13):111423.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
PVR and ICAM-1 on Blast Crisis CML Stem and Progenitor Cells with TKI Resistance Confer Susceptibility to NK Cells
Authors: N Kim, MY Kim, YU Cho, W Chen, KH Lee, HS Kim
Cancers (Basel), 2020-07-16;12(7):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Two alternate strategies for innate immunity to Epstein-Barr virus: One using NK cells and the other NK cells and ?? T cells
Authors: Z Djaoud, LA Guethlein, A Horowitz, T Azzi, N Nemat-Gorg, D Olive, D Nadal, PJ Norman, C Münz, P Parham
J. Exp. Med., 2017-05-03;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Homo-oligomerization of the activating natural killer cell receptor NKp30 ectodomain increases its binding affinity for cellular ligands.
Authors: Herrmann, Julia, Berberich, Hannah, Hartmann, Jessica, Beyer, Steffen, Davies, Karen, Koch, Joachim
J Biol Chem, 2013-11-25;289(2):765-77.
Species: Human, Insect, Moth - Trichoplusia ni (Cabbage Looper), Mouse
Sample Types: Cell Culture Supernates, Whole Cells
Applications: ELISA Development, Flow Cytometry, IHC, IHC-Fr -
Bortezomib down-regulates the cell-surface expression of HLA class I and enhances natural killer cell-mediated lysis of myeloma.
Authors: Shi J, Tricot GJ, Garg TK, Malaviarachchi PA, Szmania SM, Kellum RE, Storrie B, Mulder A, Shaughnessy JD, Barlogie B, van Rhee F
Blood, 2007-10-18;111(3):1309-17.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
A novel binding assay to assess specificity of monoclonal antibodies.
Authors: Warren HS, Warren</LastName><ForeNam HS</Initia, Bettadapura J
J. Immunol. Methods, 2005-08-15;305(1):33-8.
Species: Human
Sample Types: Complex Sample Type
Applications: Epitope Mapping -
Evidence that the cellular ligand for the human NK cell activation receptor NKp30 is not a heparan sulfate glycosaminoglycan.
Authors: Warren HS, Jones AL, Freeman C, Bettadapura J, Parish CR
J. Immunol., 2005-07-01;175(1):207-12.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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