Human NKp30/NCR3 Antibody

Catalog #: MAB18492 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB18492-100
MAB18492-SP

Detection of NKp30/NCR3 in Human PBMCs by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human NKp30/NCR3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human NKp30/NCR3 in direct ELISAs.

Source

Recombinant Monoclonal Rabbit IgG Clone # 2615A

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived human NKp30/NCR3
Leu19-Thr138
Accession # Q05D23

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

Immunohistochemistry

1-25 µg/mL

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of NKp30/NCR3 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human NCAM-1/CD56 PE-conjugated Monoclonal Antibody (Catalog # FAB2408P) and either (A) Rabbit Anti-Human NKp30/NCR3 Monoclonal Antibody (Catalog # MAB18492) or (B) Rabbit IgG Isotype Control (Catalog # MAB1050) followed by Goat anti-Rabbit IgG APC-conjugated secondary antibody (Catalog # F0111). View our protocol for Staining Membrane-associated Proteins.

Immunohistochemistry

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NKp30/NCR3 in Human Tonsil. NKp30/NCR3 was detected in immersion fixed paraffin-embedded sections of human tonsil using Rabbit Anti-Human NKp30/NCR3 Monoclonal Antibody (Catalog # MAB18492) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NKp30/NCR3

NKp30, along with NKp44 and NKp46, constitute a group of receptors termed “Natural Cytotoxicity Receptors” (1). These receptors play a major role in triggering NK-mediated killing of most tumor cells lines. NKp30 is a type I transmembrane protein having a single extracellular V-like immunoglobulin domain (2). A physical association with the ITAM‑bearing accessory protein, CD3 zeta, occurs via a charged residue in the NKp30 transmembrane domain. Ligation of NKp30 with a specific antibody results in phosphorylation of CD3 zeta (3). NKp30 is expressed on both resting and activated NK cells of the CD56^dim, CD16⁺ subset that account for more that 85% of NK cells found in peripheral blood and spleen (4). NKp30 is absent from the CD56^bright, CD16^- subset that constitutes the majority of NK cells in lymph node and tonsil, however, its expression is up-regulated in these cells upon IL-2 activation (4). Studies with neutralizing antibodies reveal that NKp30 is partially responsible for triggering lytic activity against several tumor cell types and that it is the main receptor responsible for NK-mediated lysis of immature dendritic cells (2, 5). The ligand(s) recognized by NKp30 has not been described.

References

Moretta, L. and A. Moretta (2004) EMBO J. 23:255.
Pende, D. et al. (1999) J. Exp. Med. 190:1505.
Augugliaro, R. et al. (2003) Eur. J. Immunol. 33:1235.
Ferlazzo, G. et al. (2004) J. Immunol. 172:1455.
Ferlazzo, G. et al. (2002) J. Exp. Med. 195:343.

Entrez Gene IDs

259197 (Human); 294251 (Rat); 102133932 (Cynomolgus Monkey)

Alternate Names

Activating natural killer receptor p30; CD337 antigen; CD337; CD337activating NK-A1 receptor; LY117,1C7NK-p30; lymphocyte antigen 117; natural cytotoxicity triggering receptor 3; Natural killer cell p30-related protein; NCR3; NKp30; NKp30MALS