Human NLRP6/NALP6 Alexa Fluor® 350-conjugated Antibody

Catalog # Availability Size / Price Qty
IC9145U-100UG
R&D Systems Antibodies
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Human NLRP6/NALP6 Alexa Fluor® 350-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human NLRP6/NALP6 in direct ELISAs.
Source
Monoclonal Mouse IgG2b Clone # 920631
Immunogen
E.coli-derived recombinant human NLRP6/NALP6
Lys35-Arg193
Accession # P59044
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 350 (Excitation= 346 nm, Emission= 442 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
0.25-1 µg/106 cells
Jurkat human acute T cell leukemia cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: NLRP6/NALP6

NLRP6 (Nod-Like Receptor Pyrin domain-containing protein 6), also known as NALP6, PYPAF5 and PAN3, is an 80-85 kDa cytoplasmic member of the NLRP family of proteins. Human NLRP is 892 amino acids (aa) in length, and contains an N-terminal Pyrin domain (aa 1-103), one Nacht region (aa 197-350), and five consecutive C-terminqal leucine-rich repeats (aa 462-868). It is expressed in select cell types, including eosinophils, neutrophils, CD4+ and CD8+ T cells, neurons, myofibroblasts, and intestinal goblet cells plus columnar epithelium. NLRP6 is perhaps best known for its participation in the formation of inflammasomes. Inflammasomes are oligomeric intracellular complexes that serve as detectors for "danger signals" that appear following microbial invasion or unexpected cell death. Following detection, recruited components to the complex (such as Caspase-1) are activated, and subsequently themselves serve to activate pro-IL-1 beta and IL-18. The actual detection of these signals is mediated by the NLRP family, possibly in a tissue specific manner. NLRP6 has been identified in both damaged peripheral nerve and intestinal epithelium. In goblet cells, NLRP6 drives mucus secretion; in intestinal columnar epithelium, NLRP6 acts as a homeostatic agent that promotes epithelial barrier integrity. It accomplishes this by inducing mature IL-18 secretion, a step that then reduces IL-22BP production and leads to an increase in IL-22, a cytokine known for its pro-mucosal homeostatic properties. Although most insults to the intestinal mucosa likely arise from bacteria, NLRP6 is also known to recognize dsRNA-based viruses such as norovirus, and induce the expression of antiviral cytokines such as IFN-alpha, -beta and -lambda. Over aa 35-193, human NLRP6 shares 69% aa sequence identity with mouse NLRP6.

Long Name
NLR Family, Pyrin Domain Containing 6
Entrez Gene IDs
171389 (Human); 101613 (Mouse); 171390 (Rat)
Alternate Names
Angiotensin II/Vasopressin Receptor; AVR; CLR11.4; NACHT, Leucine Rich Repeat And PYD Containing 6; NACHT, LRR And PYD Containing Protein 6; NACHT, LRR And PYD Domains-Containing Protein 6; NALP6; NAVR; NAVR/AVR; NLR Family, Pyrin Domain Containing 6; NLRP6 ; NLRP6; Nucleotide-Binding Oligomerization Domain, Leucine Rich Repeat And Pyrin Domain Containing 6; PAN3; PYPAF5; PYRIN-Containing APAF1-Like Protein 5

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Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

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