Human NLRP6/NALP6 Antibody Summary
Lys35-Arg193
Accession # P59044
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of NLRP6/NALP6 in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human NLRP6/NALP6 Monoclonal Antibody (Catalog # MAB9145, filled histogram) or isotype control antibody (Catalog # MAB004, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NLRP6/NALP6
NLRP6 (Nod-Like Receptor Pyrin domain-containing protein 6), also known as NALP6, PYPAF5 and PAN3, is an 80-85 kDa cytoplasmic member of the NLRP family of proteins. Human NLRP is 892 amino acids (aa) in length, and contains an N-terminal Pyrin domain (aa 1-103), one Nacht region (aa 197-350), and five consecutive C-terminqal leucine-rich repeats (aa 462-868). It is expressed in select cell types, including eosinophils, neutrophils, CD4+ and CD8+ T cells, neurons, myofibroblasts, and intestinal goblet cells plus columnar epithelium. NLRP6 is perhaps best known for its participation in the formation of inflammasomes. Inflammasomes are oligomeric intracellular complexes that serve as detectors for "danger signals" that appear following microbial invasion or unexpected cell death. Following detection, recruited components to the complex (such as Caspase-1) are activated, and subsequently themselves serve to activate pro-IL-1 beta and IL-18. The actual detection of these signals is mediated by the NLRP family, possibly in a tissue specific manner. NLRP6 has been identified in both damaged peripheral nerve and intestinal epithelium. In goblet cells, NLRP6 drives mucus secretion; in intestinal columnar epithelium, NLRP6 acts as a homeostatic agent that promotes epithelial barrier integrity. It accomplishes this by inducing mature IL-18 secretion, a step that then reduces IL-22BP production and leads to an increase in IL-22, a cytokine known for its pro-mucosal homeostatic properties. Although most insults to the intestinal mucosa likely arise from bacteria, NLRP6 is also known to recognize dsRNA-based viruses such as norovirus, and induce the expression of antiviral cytokines such as IFN-alpha, -beta and -lambda. Over aa 35-193, human NLRP6 shares 69% aa sequence identity with mouse NLRP6.
Product Datasheets
Citation for Human NLRP6/NALP6 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Defects in NLRP6, autophagy and goblet cell homeostasis are associated with reduced duodenal CRH receptor 2 expression in patients with functional dyspepsia
Authors: JK Bruce, GL Burns, W Sinn Soh, PM Nair, S Sherwin, K Fan, LR Dowling, BJ Goggins, N Koloski, M Potter, S Bollipo, R Foster, LT Gan, M Veysey, DJ Philpott, SE Girardin, G Holtmann, GE Kaiko, MM Walker, NJ Talley, S Keely
Brain, Behavior, and Immunity, 2022-01-31;101(0):335-345.
Species: Human
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC, Western Blot
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