Human Nrf2 Antibody Summary
Met17-Asn605
Accession # Q16236
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Nrf2 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-), treated (+) or mock treated (m) with 10 µM MG132 (Catalog # 1748) for 4 hours and 10 pmol siRNA. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Nrf2 Monoclonal Antibody (Catalog # MAB3925) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Nrf2 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Nrf2
NF-E2 related factor 2 (Nrf2) is a member of the cap ‘n’ collar family of basic leucine zipper transcription factors. By binding antioxidant response elements (AREs), Nrf2 activates the transcription of genes encoding antioxidant and phase II drug-metabolizing enzymes needed to protect cells against oxidative damage. Transcriptional targets regulated by Nrf2 include genes encoding glutathione S-transferases, superoxide dismutase-1, and heme oxygenase-1.
Product Datasheets
Citations for Human Nrf2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
9
Citations: Showing 1 - 9
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Diphenylarsinic acid induced activation of MAP kinases, transcription factors, and oxidative stress-responsive factors and hypersecretion of cytokines in cultured normal human cerebellar astrocytes
Authors: S Sasaki, T Negishi, T Tsuzuki, K Yukawa
Neurotoxicology, 2021-12-06;88(0):196-207.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
N-Acetyl Cysteine Modulates the Inflammatory and Oxidative Stress Responses of Rescued Growth-Arrested Dental Pulp Microtissues Exposed to TEGDMA in ECM
Authors: G Kaufman, D Skrtic
Int J Mol Sci, 2020-10-03;21(19):.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Selenium Nanoparticles Pre-Treatment Reverse Behavioral, Oxidative Damage, Neuronal Loss and Neurochemical Alterations in Pentylenetetrazole-Induced Epileptic Seizures in Mice
Authors: X Yuan, Z Fu, P Ji, L Guo, AO Al-Ghamdy, A Alkandiri, OA Habotta, AE Abdel Mone, RB Kassab
Int J Nanomedicine, 2020-08-24;15(0):6339-6353.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Biomarkers of Broccoli Consumption: Implications for Glutathione Metabolism and Liver Health
Authors: AA Eve, X Liu, Y Wang, MJ Miller, EH Jeffery, Z Madak-Erdo
Nutrients, 2020-08-20;12(9):.
Species: Rat
Sample Types: Protein, Tissue Homogenates
Applications: Neutralization, Western Blot -
N-Acetylcysteine Reduced Ischemia and Reperfusion Damage Associated with Steatohepatitis in Mice
Authors: N Chaves Cay, M Kiyomi Koi, JF Jacysyn, R Rasslan, AR Azevedo Ce, SK Pereira Co, JA Picanço Di, E Massazo Ut, E Frasson de
Int J Mol Sci, 2020-06-09;21(11):.
Species: Mouse
Sample Types: Nuclear Extract
Applications: Western Blot -
Protective effect of KLF15 on vascular endothelial dysfunction induced by TNF??.
Authors: Bing Liu, Lili Xu, Xinming Yu, Wei Li, Xiaozhi Sun, Shun Xiao, Mingjin Guo, Haofu Wang
Molecular Medicine Reports, 2018-06-20;0(0):1791-3004.
Species: Human
Sample Types: Cell Lysate, Cell Lysates
Applications: Western Blot -
Cervical Cancer Cell Line Secretome Highlights the Roles of Transforming Growth Factor-Beta-Induced Protein ig-h3, Peroxiredoxin-2, and NRF2 on Cervical Carcinogenesis
Authors: G Kontostath, J Zoidakis, M Makridakis, V Lygirou, G Mermelekas, T Papadopoul, K Vougas, A Vlamis-Gar, P Drakakis, D Loutradis, A Vlahou, NP Anagnou, KI Pappa
Biomed Res Int, 2017-02-02;2017(0):4180703.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Vitiligo-inducing phenols activate the unfolded protein response in melanocytes resulting in upregulation of IL6 and IL8.
J. Invest. Dermatol., 2012-06-14;132(11):2601-9.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Dimethyl fumarate, an immune modulator and inducer of the antioxidant response, suppresses HIV replication and macrophage-mediated neurotoxicity: a novel candidate for HIV neuroprotection.
Authors: Cross SA, Cook DR, Chi AW, Vance PJ, Kolson LL, Wong BJ, Jordan-Sciutto KL, Kolson DL
J. Immunol., 2011-10-05;187(10):5015-25.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
FAQs
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***Bio-Techne Response: In recent specificity testing, RnD Systems has determined this product does not recognize mouse and rat Nrf2 and is not recommended for those species. If you are testing mouse samples, consider AF3925. If you are testing rat samples, consider NBP1-32822 from Novus Biologicals.***
***Bio-Techne Response: In recent specificity testing, RnD Systems has determined this product does not recognize mouse and rat Nrf2 and is not recommended for those species. If you are testing mouse samples, consider AF3925. If you are testing rat samples, consider NBP1-32822 from Novus Biologicals.***
RMC-1 cells, rat Muller cells, were cultured and treated, collect with NP-40 buffer, run WB. The problem is the molecular weight, we only see two specific bands around 75 KD marker, we didn't see any band around 120Kd as datasheet from RnD even longer exposure time. We would like to know why. I would like to provide more detailed information if you want to know. Thanks a lot.
***Bio-Techne Response: In recent specificity testing, RnD Systems has determined this product does not recognize mouse and rat Nrf2 and is not recommended for those species. If you are testing mouse samples, consider AF3925. If you are testing rat samples, consider NBP1-32822 from Novus Biologicals.***
