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Human NRG1‑ alpha /HRG1‑ alpha EGF Domain Antibody

Catalog #: AF-296-NA
5 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF-296-NA
AF-296-SP
NRG1-alpha /HRG1-alpha in Human Breast.
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Citations (5)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human NRG1‑ alpha /HRG1‑ alpha EGF Domain Antibody Summary

Species Reactivity
Human
Specificity
Detects human NRG1-alpha /HRG1-alpha in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human NRG1-alpha /HRG1-alpha
Ser177-Lys241
Accession # Q7RTV8
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Purity
Immunogen affinity purified

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human NRG1-alpha /HRG1-alpha EGF Domain (Catalog # 296-HR)
Immunohistochemistry
5-15 µg/mL
See below
Neutralization
Measured by its ability to neutralize NRG1‑ alpha /HRG1‑ alpha -induced proliferation in the MCF‑7 human breast cancer cell line. The Neutralization Dose (ND50) is typically 7-30 µg/mL in the presence of 125 ng/mL Recombinant Human NRG1‑ alpha /HRG1‑ alpha EGF Domain.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry NRG1-a/HRG1-a antibody in Human Breast by Immunohistochemistry (IHC-P). View Larger

NRG1-alpha /HRG1-alpha in Human Breast. NRG1-a/HRG1-a was detected in paraffin-embedded sections of human breast using Goat Anti-Human NRG1-a/HRG1-a EGF Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-296-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS009) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Neutralization Cell Proliferation Induced by NRG1‑ alpha /HRG1‑ alpha and Neutralization by Human NRG1‑ alpha /HRG1‑ alpha Antibody. View Larger

Cell Proliferation Induced by NRG1‑ alpha /HRG1‑ alpha and Neutralization by Human NRG1‑ alpha /HRG1‑ alpha Antibody. Recombinant Human NRG1-a/HRG1-a EGF Domain (Catalog # 296-HR) stimulates proliferation in the MCF-7 human breast cancer cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human NRG1-a/HRG1-a EGF Domain (125 ng/mL) is neutralized (green line) by increasing concentrations of Human NRG1-a/HRG1-a EGF Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-296-NA). The ND50 is typically 7-30 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neuregulin-1 alpha/NRG1 alpha

The neuregulin family of structurally related glycoproteins comprises products from four distinct but related genes, Nrg-1, Nrg-2, Nrg-3, and Nrg-4. Through alternative splicing or the use of alternative promoters, Nrg-1 encodes more than 14 soluble or transmembrane proteins. The extracellular domain of the transmembrane NRG1 isoforms can be proteolytically cleaved to release soluble growth factors. The alpha - or  beta -splice variants differ in their C-terminal region. All NRG1 isoforms contain an EGF-like domain that is required for their direct binding to the ErbB3 or ErbB4 receptor tyrosine kinases. The ErbB3 or ErbB4 subsequently recruits and heterodimerizes with ErbB2, resulting in tyrosine phosphorylation and NRG1 signaling. NRG1 isoforms can be classified into three major subtypes. Type I (Neu Differentiation Factor, NDF; Heregulin, HRG; Acetylcholine Receptor Inducing Activity, ARIA) and type II (Glial Growth Factor, GGF). NRG1s have an immunoglobulin (Ig)-like domain N‑terminal to the EGF-like domain. Type I NRG1s differ from type II NRG1s by having a glycosylation-rich domain between the Ig-like and the EGF-like domains. Type III NRG1 (Sensory and Motor neuron-Derived Factor) lacks the Ig-like domain but has a cysteine rich domain (CRD) instead. NRG1 isoforms show distinct spatial and temporal expression patterns. These proteins play important roles during development of both the nervous system and the heart. They have been shown to regulate the selective expression of neurotransmitter receptors in neurons and at the neuromuscular junction, and to promote the differentiation and development of Schwann cells from neural crest stem cells. NRG1s have also been shown to be involved in the establishment of the oligodendroglial lineage.

References
  1. Lemke, G. (2006) Science STKE 325:pe11.
  2. Esper, R.M. et al. (2006) Brain Res. Brain Res. Rev. 51:161.
  3. Tosato, S. et al. (2005) Schizophr. Bull. 31:613.
Entrez Gene IDs
3084 (Human); 211323 (Mouse); 112400 (Rat)
Alternate Names
ARIA; GGF2; HGL; HRG; HRG1; MST131; MSTP131; neuregulin 1; Neuregulin1 alpha; Neuregulin-1 alpha; pro-neuregulin-1, membrane-bound isoform; pro-NRG1; sensory and motor neuron derived factor; SMDF

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Citations for Human NRG1‑ alpha /HRG1‑ alpha EGF Domain Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Anti-Trop2 blockade enhances the therapeutic efficacy of ErbB3 inhibition in head and neck squamous cell carcinoma
    Authors: N Redlich, AM Robinson, KP Nickel, AP Stein, DL Wheeler, DR Adkins, R Uppaluri, RJ Kimple, BA Van Tine, LS Michel
    Cell Death Dis, 2018-01-05;9(1):5.
    Species: Xenograft
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  2. Type I neuregulin1? is a novel local mediator to suppress hepatic gluconeogenesis in mice
    Authors: T Arai, Y Ono, Y Arimura, K Sayama, T Suzuki, S Shinjo, M Kanai, SI Abe, K Semba, N Goda
    Sci Rep, 2017-02-20;7(0):42959.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  3. Neuregulin autocrine signaling promotes self-renewal of breast tumor-initiating cells by triggering HER2/HER3 activation.
    Authors: Lee, Cleo Yi-, Lin, Yuan, Bratman, Scott V, Feng, Weiguo, Kuo, Angera H, Scheeren, Ferenc A, Engreitz, Jesse M, Varma, Sushama, West, Robert B, Diehn, Maximili
    Cancer Res, 2013-10-31;74(1):341-52.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  4. Glial growth factor 2 promotes functional recovery with treatment initiated up to 7 days after permanent focal ischemic stroke.
    Authors: Iaci JF, Ganguly A, Finklestein SP, Parry TJ, Ren J, Saha S, Sietsma DK, Srinivas M, Vecchione AM, Caggiano AO
    Neuropharmacology, 2010-08-04;59(7):640-9.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Western Blot
  5. HER family receptor abnormalities in lung cancer brain metastases and corresponding primary tumors.
    Authors: Sun M, Behrens C, Feng L, Ozburn N, Tang X, Yin G, Komaki R, Varella-Garcia M, Hong WK, Aldape KD, Wistuba II
    Clin. Cancer Res., 2009-07-21;15(15):4829-37.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P

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