Human NT-4 Antibody Summary
Gly81-Ala210 (predicted)
Accession # P34130
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NT-4
Neurotrophin-4 (NT-4), also known as NT-5, is a member of the NGF family of neuronal and epithelial growth factors. Neurotrophins have six conserved cysteine residues that are involved in the formation of three disulfide bonds (1‑3). The human NT-4 cDNA encodes a 210 amino acid (aa) precursor that includes a 24 aa signal sequence, a 56 aa propeptide, and a 130 aa mature protein (4, 5). NT-4 is synthesized as a 28 kDa prepropeptide that is proteolytically processed to generate the mature protein. Mature human NT-4 shares 48‑52% aa sequence identity with human beta-NGF, BDNF, and NT-3. It shares 91% and 95% aa sequence identity with mouse and rat NT-4/5, respectively. The mature protein is secreted as a homodimer and can also form heterodimers with BDNF or NT-3 (6). NT-4 binds and induces receptor dimerization and activation of TrkB (4, 7). NT-4 promotes the development and survival of selected peripheral and CNS neurons (8‑10). BDNF, which also activates TrkB, overlaps with many but not all NT-4 functions, a distinction that is likely due to differences in expression patterns (8‑10). NT-4 induced TrkB signaling augments NMDA receptor activity and increases neuronal sensitivity to excitotoxic cell death (11). It also promotes the proliferation of keratinocytes and accelerates hair follicle regression during the follicular cycle (12, 13). NT-4 is secreted by activated T cells and granulocytes at sites of inflammation where it contributes to tissue regeneration (14‑16).
- Lessmann, V. et al. (2003) Prog. Neurobiol. 69:341.
- Tabakman, R. et al. (2004) Prog. Brain Res. 146:387.
- Botchkarev, V.A. et al. (2004) Prog. Brain Res. 146:493.
- Ip, N.Y. et al. (1992) Proc. Natl. Acad. Sci. 89:3060.
- Berkemeier, L.R. et al. (1991) Neuron 7:857.
- Radziejewski, C. and R.C. Robinson (1993) Biochemistry 32:13350.
- Vesa, J. et al. (2000) J. Biol. Chem. 275:24414.
- Davies, A.M. et al. (1993) J. Neurosci. 13:4961.
- Stucky, C.L. et al. (1998) J. Neurosci. 18:7040.
- Fan, G. et al. (2000) Nat. Neurosci. 3:350.
- Choi, S.Y. et al. (2004) J. Neurochem. 88:708.
- Botchkarev, V.A. et al. (1999) Lab. Invest. 79:557.
- Botchkarev, V.A. et al. (1999) FASEB J. 13:395.
- Laurenzi, M.A. et al. (1998) J. Leukoc. Biol. 64:228.
- Moalem, G. et al. (2000) J. Autoimmun. 15:331.
- Nassenstein, C. et al. (2003) J. Exp. Med. 198:455.
Product Datasheets
Citation for Human NT-4 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Neuropeptide Y fragments derived from neprilysin processing are neuroprotective in a transgenic model of Alzheimer's disease.
Authors: Rose JB, Crews L, Rockenstein E, Adame A, Mante M, Hersh LB, Gage FH, Spencer B, Potkar R, Marr RA, Masliah E
J. Neurosci., 2009-01-28;29(4):1115-25.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot
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