Human OX40 Ligand/TNFSF4 Antibody Summary
Gln51-Leu183
Accession # Q6FGS4
Applications
This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human OX40/TNFRSF4 Monoclonal Antibody (Catalog # MAB10544).
This product is intended for assay development on various assay platforms requiring antibody pairs.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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IL-8 Secretion Induced by OX40 Ligand/TNFSF4 and Neutralization by Human OX40 Ligand/TNFSF4 Antibody. Recombinant Human OX40 Ligand/TNFSF4 (Catalog # 1054-OX) induces IL-8 secretion in the HT1080 human fibrosarcoma cell line transfected with human OX40 in a dose-dependent manner as measured by the Human IL-8 DuoSet (Catalog # DY208). IL-8 secretion elicited by Recombinant Human OX40 Ligand/TNFSF4 (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human OX40 Ligand/TNFSF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1054). The ND50 is typically 0.015-0.09 µg/mL.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: OX40 Ligand/TNFSF4
OX40 Ligand (OX40L), also known as gp34, is a type II transmembrane glycoprotein belonging to the TNF superfamily. Human OX40L cDNA encodes a 183 amino acids (aa) polypeptide with an amino-terminal cytoplasmic domain (aa 1-23) and a carboxy-terminal extracellular domain (aa 51-183). It shares 46% aa sequence identity with the mouse counterpart. OX40L is expressed on the surface of activated B cells, T cells, dendritic cells, and endothelial cells. Similarly to other TNF superfamily members, membrane-bound OX40 Ligand exists as a homotrimer. OX40L binds to OX40 (CD134), a member of the TNF receptor superfamily that is expressed predominantly on activated CD4+ T cells. OX40 Ligand is one of the co-stimulatory molecules in the immune system that includes B7, CD40 Ligand, CD30 Ligand, CD27 Ligand, and 4-1BB Ligand. Because OX40 appears as a late activation-induced T cell surface antigen, it has been speculated that the major function of OX40-OX40L interaction is to transmit a late co-stimulatory signal to promote the survival and proliferation of activated CD4+ T cells and prolong the immune response. Engagement of OX40 on activated T cells in situ in tumors has been shown to augment immune responses and subsequent tumor regression.
- Godfrey, W.R. et al. (1994) J. Exp. Med. 180:757.
- Baum, P.R. et al. (1994) EMBO J. 13:3992.
- Al-Shamkhani, A. et al. (1997) J. Biol. Chem. 272:5275.
- Kjaergaard, J. et al. (2000) Cancer Res. 60:5514.
Product Datasheets
Citations for Human OX40 Ligand/TNFSF4 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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CD40 Enhances Type I Interferon Responses Downstream of CD47 Blockade, Bridging Innate and Adaptive Immunity
Authors: S de Silva, G Fromm, CW Shuptrine, K Johannes, A Patel, KJ Yoo, K Huang, TH Schreiber
Cancer Immunol Res, 2019-12-18;0(0):.
Species: Human, Mouse
Sample Types: Protein
Applications: Western Blot -
HDAC11 plays an essential role in regulating OX40 ligand expression in Hodgkin lymphoma.
Authors: Buglio D, Khaskhely NM, Voo KS, Martinez-Valdez H, Liu YJ, Younes A
Blood, 2011-01-14;117(10):2910-7.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Mapping the domains of CD134 as a functional receptor for feline immunodeficiency virus.
Authors: Willett BJ, McMonagle EL, Bonci F, Pistello M, Hosie MJ
J. Virol., 2006-08-01;80(15):7744-7.
Species: Feline
Sample Types: Whole Cells
Applications: Flow Cytometry
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