Human p21/CIP1/CDKN1A Alexa Fluor® 700-conjugated Antibody Summary
Ser2-Pro164
Accession # P38936
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
Detection of p21/Cip1/CDKN1A in MCF‑7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line either treated with 1 µM camptothecin (CPT) for 16 hours (filled histogram) or untreated (open histogram) was stained with Alexa Fluor® 700-conjugated Mouse Anti-Human p21/CIP1/CDKN1A Monoclonal Antibody (Catalog # IC1047N). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: p21/CIP1/CDKN1A
p21CIP1, also called CIP1 (CDK-interacting protein 1) and CDKN1A, is a 21 kDa Cyclin/Cyclin-dependent kinase (Cdk) inhibitor that blocks cell cycle progression from G1 to S phase in the cell cycle. Because p21 is a transcriptional target of the p53 tumor suppressor, p21 expression increases in cells that contain stabilized p53 due to genotoxic stress exposure.
Product Datasheets
Product Specific Notices
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Human p21/CIP1/CDKN1A Alexa Fluor® 700-conjugated Antibody
Average Rating: 5 (Based on 1 Review)
Have you used Human p21/CIP1/CDKN1A Alexa Fluor® 700-conjugated Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Filter by:
