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Human p63/TP73L Antibody

Catalog #: AF1916
10 Citations 3 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1916
AF1916-SP
p63/TP73L in Human Breast.
2 Images
Product Details
Citations (10)
FAQs
Supplemental Products
Reviews (3)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human p63/TP73L Antibody Summary

Species Reactivity
Human
Specificity
Detects human p63/TP73L in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human p63/TP73L
Met40-Cys339
Accession # Q9H3D4
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human p63/TP73L
Immunohistochemistry
5-15 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry p63/TP73L antibody in Human Breast by Immunohistochemistry (IHC-P). View Larger

p63/TP73L in Human Breast. p63/TP73L was detected in immersion fixed paraffin-embedded sections of human breast using Goat Anti-Human p63/TP73L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1916) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunocytochemistry p63/TP73L antibody in SCC-25 Human Cell Line by Immunocytochemistry (ICC). View Larger

p63/TP73L in SCC-25 Human Cell Line. p63/TP73L was detected in immersion fixed SCC-25 human tongue carcinoma cell line using Goat Anti-Human p63/TP73L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1916) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: p63/TP73L

Tumor Protein 63 (p63), also named TP73L, TP63, p51, p40 or KET, is a p53 homolog. It is one of several proteins that are produced from a single gene using two promoters and alternative splicing of the primary RNA transcript. p63 is highly expressed in embryonic ectoderm and in the nuclei of basal regenerative cells of many epithelial tissues in the adult. p63 is suggested to play a role in development, epithelial cell maintenance and tumorigenesis (1‑3).

References
  1. Harms, K. et al. (2004), Cell Mol. Life Sci. 61(7):822.
  2. Koster, M.I. and D.R. Roop, J. Dermatol. Sci. 34(1):3.
  3. Benard, J. et al. (2003) Hum. Mutat. 21(3):182.
Long Name
Tumor Protein 63
Entrez Gene IDs
8626 (Human)
Alternate Names
amplified in squamous cell carcinoma; Chronic ulcerative stomatitis protein; CUSP; EEC3; EEC3B(p51B); Keratinocyte transcription factor KET; KET; KETRHS; LMS; OFC8; OFC8SHFM4TP73LNBP; p40; p51; p51tumor protein p53-like; p53CP; p63; p63AIS; p73H; p73L; p73LB(p51A); SHFM4; TP53CP; TP53L; TP63; TP73L; Transformation-related protein 63; tumor protein 63; tumor protein p53-competing protein; tumor protein p63 deltaN isoform delta; tumor protein p63; Tumor protein p73-like

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Citations for Human p63/TP73L Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Human archetypal pluripotent stem cells differentiate into trophoblast stem cells via endogenous BMP5/7 induction without transitioning through naive state
    Authors: Tietze, E;Barbosa, AR;Araujo, B;Euclydes, V;Spiegelberg, B;Cho, HJ;Lee, YK;Wang, Y;McCord, A;Lorenzetti, A;Feltrin, A;van de Leemput, J;Di Carlo, P;Ursini, G;Benjamin, KJ;Brentani, H;Kleinman, JE;Hyde, TM;Weinberger, DR;McKay, R;Shin, JH;Sawada, T;Paquola, ACM;Erwin, JA;
    Scientific reports
    Species: Human hepegivirus
    Sample Types: Whole Cells
    Applications: Immunocytochemistry
  2. Combined Mek inhibition and Pparg activation Eradicates Muscle Invasive Bladder cancer in a Mouse Model of BBN-induced Carcinogenesis
    Authors: Tate, T;Plumber, SA;Al-Ahmadie, H;Chen, X;Choi, W;Lu, C;Viny, A;Batourina, E;Gartensson, K;Alija, B;Molotkov, A;Wiessner, G;McKiernan, J;McConkey, D;Dinney, C;Czerniak, B;Mendelsohn, CL;
    bioRxiv : the preprint server for biology
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  3. Differential susceptibility to SARS-CoV-2 in the normal nasal mucosa and in chronic sinusitis
    Authors: Z Zhang, H Peng, J Lai, L Jiang, L Wang, S Jin, K Fan, Z Zhang, C Zhao, D Deng, P Zhao, Z Gao, S Yu
    European Journal of Immunology, 2022-05-14;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  4. Functional roles for Piezo1 and Piezo2 in urothelial mechanotransduction and lower urinary tract interoception
    Authors: MG Dalghi, WG Ruiz, DR Clayton, N Montalbett, SL Daugherty, JM Beckel, MD Carattino, G Apodaca
    JCI Insight, 2021-10-08;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Nicotinic Receptor Subunits Atlas in the Adult Human Lung
    Authors: Z Diabasana, JM Perotin, R Belgacemi, J Ancel, P Mulette, G Delepine, P Gosset, U Maskos, M Polette, G Deslée, V Dormoy
    Int J Mol Sci, 2020-10-09;21(20):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  6. Sonic hedgehog signalling as a potential endobronchial biomarker in COPD
    Authors: J Ancel, R Belgacemi, JM Perotin, Z Diabasana, S Dury, M Dewolf, A Bonnomet, N Lalun, P Birembaut, M Polette, G Deslée, V Dormoy
    Respir. Res., 2020-08-07;21(1):207.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  7. Chromosome 3q26 Gain Is an Early Event Driving Coordinated Overexpression of the PRKCI, SOX2, and ECT2 Oncogenes in Lung Squamous Cell Carcinoma
    Authors: Y Liu, N Yin, X Wang, A Khoor, V Sambandam, AB Ghosh, ZA Fields, NR Murray, V Justilien, AP Fields
    Cell Rep, 2020-01-21;30(3):771-782.e6.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. The oncogenic and druggable hPG80 (Progastrin) is overexpressed in multiple cancers and detected in the blood of patients
    Authors: B You, F Mercier, E Assenat, C Langlois-J, O Glehen, J Soulé, L Payen, V Kepenekian, M Dupuy, F Belouin, E Morency, V Saywell, M Flacelière, P Elies, P Liaud, T Mazard, D Maucort-Bo, W Tan, B Vire, L Villeneuve, M Ychou, M Kohli, D Joubert, A Prieur
    EBioMedicine, 2019-12-24;51(0):102574.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  9. Airway epithelial cell differentiation relies on deficient Hedgehog signalling in COPD
    Authors: R Belgacemi, E Luczka, J Ancel, Z Diabasana, JM Perotin, A Germain, N Lalun, P Birembaut, X Dubernard, JC Mérol, G Delepine, M Polette, G Deslée, V Dormoy
    EBioMedicine, 2019-12-23;51(0):102572.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  10. p73 regulates epidermal wound healing and induced keratinocyte programming
    Authors: JS Beeler, CB Marshall, PI Gonzalez-E, TM Shaver, GL Santos Gua, ST Lea, KN Johnson, H Jin, BJ Venters, ME Sanders, JA Pietenpol
    PLoS ONE, 2019-06-19;14(6):e0218458.
    Species: Human
    Sample Types: Cell Lysates
    Applications: ChIP

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Reviews for Human p63/TP73L Antibody

Average Rating: 4 (Based on 3 Reviews)

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Human p63/TP73L Antibody
By Michele Torino on 12/07/2017
Application: WB Sample Tested: 293T human embryonic kidney cell line Species: Human
Human p63/TP73L Antibody
By Anonymous on 05/16/2017
Application: WB Sample Tested: Mammary gland tissue Species: Human
Human p63/TP73L Antibody
By Anonymous on 12/15/2016
Application: ICC/IF Sample Tested: Prostate cancer Species: Human

p63 is prostate stem cell marker. i have isolated stem cells from BPH patients and stained with p63 marker. I found excellent results with DAB kit and also p63 antibody performed superb job.