Human PAG1 Antibody Summary
Ser38-Leu432
Accession # Q9NWQ8
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
Detection of Human PAG1 by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human PAG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5285) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PAG1 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PAG1
PAG1 (Phosphoprotein-associated with glycosphingolipid-enriched microdomains 1; also Cbp/Csk-binding protein) is a 47 kDa protein that runs anomalously at 70‑85 kDa in SDS-PAGE. PAG1 is a constitutively phosphorylated and palmitoylated protein that occurs in leukocyte lipid rafts. It negatively regulates Src family kinases by linking Csk to FynT. Human PAG1 is 432 amino acids (aa) in length. It is a type III (no signal sequence) transmembrane protein that contains a 16 aa extracellular region (aa 31‑16) and a 395 aa cytoplasmic domain (aa 38‑432). PAG1 is palmitoylated at Cys37 and phosphorylated at Tyr317 where it interacts with Csk. There is an FynT binding site at aa 131‑137. One splice variant shows a 36 aa substitution for aa 293‑432. Over aa 38‑432, human PAG1 is 79% aa identical to mouse PAG1.
Product Datasheets
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