Human Pappalysin‑1/PAPP-A Biotinylated Antibody

Catalog #: BAF2487 Datasheet / COA / SDS

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BAF2487

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Pappalysin‑1/PAPP-A Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Pappalysin-1/PAPP-A in Western blots. In this format, less than 5% cross-reactivity with recombinant human PAPP‑A2 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Pappalysin-1/PAPP-A (R&D Systems, Catalog # 2487-ZN)
Glu82-Asp1214
Accession # Q13219

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Pappalysin‑1/PAPP-A (Catalog # 2487-ZN)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Pappalysin-1/PAPP-A

Pappalysins belong to a fifth family of metzincins that consists of ADAMs/ADAMTSs, MMPs, astacins and serrylysins (1). PAPP-A is an important pregnancy protein and increases in plasma by a factor of about 150 during pregnancy as compared to the nonpregnant state. PAPP-A is also a major marker of Down syndrome in the first trimester of pregnancy because maternal serum levels of PAPP-A are significantly reduced when a fetus affected by Down syndrome is present (2). PAPP-A cleaves Insulin-like Growth Factor-Binding Protein-4 and -5 (IGFBP-4 and -5) at a single site, resulting in the release of bioactive IGF (3). Lack of IGFBP-4 cleavage in embryonic fibroblasts derived from PAPP-A knockout mice indicates that PAPP-A functions as a physiological IGFBP-4 protease (4). Three Lin12-Notch repeats (LNR) in the PAPP-A protein bind Ca²⁺ and are required for the cleavage of IGFBP-4, not IGFBP-5, by PAPP-A (5). The C-terminal LNR (residues 1476 to 1503) is not present in rhPAPP-A (residues 82 to 1214), which starts at the N-terminus of the mature chain and ends before the five Sushi (SCR) modules. As an active protease, rhPAPP-A cleaves IGFBP-5, which can be inhibited by 1,10-phenanthroline.

References

Boldt, H.B. et al. (2001) Biochem. J. 358:359.
Fialova L. and I.M. Malbohan (2002) Bratisl. Lek. Listy 103:194.
Laursen, L.S. et al. (2001) FEBS Lett. 504:36.
Conover, C.A. et al. (2004) Development 131:1187.
Boldt, H.B. et al. (2004) J. Biol. Chem. 279:38525.

Long Name

Pregnancy-associated Plasma Protein 1

Entrez Gene IDs

5069 (Human); 18491 (Mouse)

Alternate Names

ASBABP2; DIPLA1; EC 3.4.24.79; IGFBP-4ase; IGFBP-4aseaspecific BCL2 ARE-binding protein 2; IGF-dependent IGFBP-4 protease; insulin-like growth factor-dependent IGF binding protein-4 protease; Insulin-like growth factor-dependent IGF-binding protein 4 protease; PAPA; PAPPA; PAPPA1; PAPP-A1; PAPP-Adifferentially placenta 1 expressed protein; Pappalysin1; Pappalysin-1; pregnacy-associated plasma protein A; Pregnancy-associated plasma protein A; pregnancy-associated plasma protein A, pappalysin 1