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Human Pappalysin-2/PAPP-A2 Antibody

Catalog #: AF1668
8 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1668
AF1668-SP
Product Details
Citations (8)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Pappalysin-2/PAPP-A2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Pappalysin‑2/PAPP‑A2 in direct ELISAs and Western blots. In Western blots, less than 1% cross‑reactivity with recombinant human PAPP-A is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Pappalysin‑2/PAPP‑A2
Ser234-Cys1396
Accession # Q9BXP8
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Pappalysin‑2/PAPP-A2 (Catalog # 1668-ZN)
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Pappalysin‑2/PAPP-A2 (Catalog # 1668-ZN), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Pappalysin-2/PAPP-A2

Pappalysins belong to a fifth family of metzincins that consists of ADAMs/ADAMTSs, MMPs, astacins and serrylysins (1, 2). PAPP-A is an important pregnancy protein and increases in plasma by a factor of about 150 during pregnancy as compared to the nonpregnant state. PAPP-A is also a major marker of Down syndrome in the first trimester of pregnancy because maternal serum levels of PAPP-A are significantly reduced when a fetus affected by Down syndrome is present (3). PAPP-A cleaves Insulin-like Growth Factor-Binding Protein-4 and -5 (IGFBP-4 and -5) at a single site, resulting in the release of bioactive IGF (4). Compared to PAPP‑A, PAPP-A2 (also called PAPP-E), the second member of the family is less characterized. PAPP-A2 shares 45% amino acid identity to PAPP-A in the mature form, which is synthesized as a preproprotein consisting of multiple domains (1). The prepro region (residues 1‑233) and the C-terminal region (residues 1397‑1791) are not included in recombinant human PAPP-A2. As an active protease, recombinant human PAPP-A2 cleaves IGFBP-5.

References
  1. Overgaard, M.T. et al. (2001) J. Biol. Chem. 276:21849.
  2. Boldt, H.B. et al. (2001) Biochem. J. 358:359.
  3. Fialova L. and I.M. Malbohan (2002) Bratisl. Lek. Listy 103:194.
  4. Laursen, L.S. et al. (2001) FEBS Lett. 504:36.
Long Name
Pregnancy-associated Plasma Protein
Entrez Gene IDs
60676 (Human)
Alternate Names
EC 3.4.24.-; PAPPA2; PAPP-A2; PAPP-A2pappalysin-2; pappalysin 2; Pappalysin2; Pappalysin-2; PAPPE; PAPP-E; PLAC3; placenta-specific 3; pregnancy-associated plasma preproprotein-A2; Pregnancy-associated plasma protein A2; Pregnancy-associated plasma protein E1

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Citations for Human Pappalysin-2/PAPP-A2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Unique Transcriptional Programs Identify Subtypes of AKI
    Authors: Katherine Xu, Paul Rosenstiel, Neal Paragas, Christian Hinze, Xiaobo Gao, Tian Huai Shen et al.
    Journal of the American Society of Nephrology
  2. IGFBP-4 and -5 are expressed in first-trimester villi and differentially regulate the migration of HTR-8/SVneo cells.
    Authors: Crosley, Erin J, Dunk, Caroline, Beristain, Alexande, Christians, Julian K
    Reprod Biol Endocrinol, 2014-12-04;12(0):123.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  3. First-Trimester Levels of Pregnancy-Associated Plasma Protein A2 (PAPP-A2) in the Maternal Circulation Are Elevated in Pregnancies That Subsequently Develop Preeclampsia
    Authors: Erin J. Crosley, Ursula Durland, Ken Seethram, Scott MacRae, Andrée Gruslin, Julian K. Christians
    Reproductive Sciences
  4. Pregnancy Associated Plasma Protein A2 (PAPP-A2) Affects Bone Size and Shape and Contributes to Natural Variation in Postnatal Growth in Mice
    Authors: Julian Kenneth Christians, Devin Rhys de Zwaan, Sunny Ho Yeung Fung
    PLoS ONE
  5. Regulation of pregnancy-associated plasma protein A2 (PAPPA2) in a human placental trophoblast cell line (BeWo)
    Authors: Pamela K Wagner, Aki Otomo, Julian K Christians
    Reproductive Biology and Endocrinology
  6. Altered placental expression of PAPPA2 does not affect birth weight in mice
    Authors: Pamela K Wagner, Julian K Christians
    Reproductive Biology and Endocrinology
  7. Expression of pregnancy-associated plasma protein A2 during pregnancy in human and mouse.
    Authors: Wang J, Qiu Q, Haider M, Bell M, Gruslin A, Christians JK
    J. Endocrinol., 2009-05-27;202(3):337-45.
    Species: Human, Mouse
    Sample Types: Serum, Whole Tissue
    Applications: IHC-P, Western Blot
  8. Increased levels of pregnancy-associated plasma protein-A2 in the serum of pre-eclamptic patients.
    Authors: Nishizawa H, Pryor-Koishi K, Suzuki M
    Mol. Hum. Reprod., 2008-09-18;14(10):595-602.
    Species: Human
    Sample Types: Serum, Tissue Homogenates, Whole Tissue
    Applications: ELISA Development, IHC-P, Western Blot

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