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Human Pax4 Antibody

Catalog #: AF2614
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2614
AF2614-SP
Product Details
Citations (2)
FAQs
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Pax4 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Pax4 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) Pax3 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Pax4
Met1-Glu343
Accession # NP_006184
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Purity
Immunogen affinity purified

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Pax4
Immunocytochemistry
5-15 µg/mL
Immersion fixed beta TC-6 mouse beta cell insulinoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Pax4

Pax4 is a member of the paired box family of transcription factors. Members of the Pax family typically contain a paired box domain and a paired-type homeodomain. These genes play critical roles during fetal development. Pax4 is known to be a key regulator for pancreatic cell differentiation and development.

Long Name
Paired Box Gene 4
Entrez Gene IDs
5078 (Human)
Alternate Names
KPD; MGC129960; MODY9paired box protein Pax-4; paired box 4; paired box gene 4; paired domain gene 4; Pax4

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Citations for Human Pax4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. The temporal and hierarchical control of transcription factors-induced liver to pancreas transdifferentiation.
    Authors: Berneman-Zeitouni D, Molakandov K, Elgart M, Mor E, Fornoni A, Dominguez M, Kerr-Conte J, Ott M, Meivar-Levy I, Ferber S
    PLoS ONE, 2014-02-04;9(2):e87812.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. Overexpression of PAX4 reduces glucagon expression in differentiating hESCs.
    Authors: Gage B, Baker R, Kieffer T
    Islets, 2014-01-01;6(2):e29236.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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