Human Pax6 PE-conjugated Antibody

Catalog #: IC8150P Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
IC8150P-100

Detection of Pax6 in HeLa Human Cell Line by Flow Cytometry.

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Human Pax6 PE-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Pax6 in direct ELISAs. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) Pax1, rhPax2, rhPax3, rhPax4, rhPax5, and rhPax7 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Pax6
Met1-Arg272
Accession # P26367

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of Pax6 antibody in HeLa Human Cell Line antibody by Flow Cytometry.

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Detection of Pax6 in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Sheep Anti-Human Pax6 PE-conjugated Affinity-Purified Polyclonal Antibody (Catalog # IC8150P, filled histogram) or Sheep IgG PE-conjugated control Antibody (Catalog # IC016P, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Pax6

Pax6 (paired box 6; also Oculorhombin) is a 48-50 kDa member of the paired homeobox family of transcription factors. It is expressed in developing optic vesicle, olfactory dopaminergic neurons, and pancreatic endocrine cells. Pax6 is a transactivating protein that interacts with MAF, CDX2 and SOX2. Human Pax6 is 422 amino acids (aa) in length. It contains an N-terminal paired box DNA-binding domain (aa 4-130), a Gly-rich central region (aa 131-209), a homeodomain (aa 213-269) and a C-terminal Pro/Ser/Thr-rich regulatory domain (aa 279-422). Phosphorylation of the C-terminal domain at Thr281/304/373 promotes Pax6 activity. Multiple splice forms of Pax6 exist. There are alternative start sites at Met137 and a position 34 aa upstream of the standard site. There is also a deletion of aa 22-26 and 37‑39, plus a 14 aa insertion after Gln47 that generates a C-terminal DNA binding site. Human and mouse Pax6 are absolutely identical in aa sequence.

Long Name

Paired Box Gene 6

Entrez Gene IDs

5080 (Human); 18508 (Mouse); 25509 (Rat)

Alternate Names

keratitis); MGC17209; Oculorhombin; paired box 6; paired box protein Pax-6; Pax6