Human Phospho-eNOS (S1177) Antibody Summary
Accession # P29474
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human eNOS by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells untreated (-) or treated (+) with 100 µM Pervanadate (PV) for 10 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Phospho-eNOS (S1177) Monoclonal Antibody (Catalog # MAB9028) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for eNOS at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Human eNOS by Simple WesternTM. Simple Western lane view shows lysates of HUVEC human umbilical vein endothelial cells untreated (-) or treated (+) with Pervanadate (PV), loaded at 0.2 mg/mL. A specific band was detected for eNOS at approximately 141 kDa (as indicated) using 10 µg/mL of Rabbit Anti-Human Phospho-eNOS (S1177) Monoclonal Antibody (Catalog # MAB9028). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: eNOS
Endothelial NOS (eNOS), also known as nitric oxide synthase 3 (NOS3) or constitutive NOS (cNOS), is an enzyme encoded by the NOS3 gene. Endothelial NOS generates nitric oxide in blood vessels and is involved with regulating vascular tone by inhibiting smooth muscle contraction and platelet aggregation. A constitutive calcium dependent NOS provides a basal release of NO. eNOS is associated with plasma membranes surrounding cells and the membranes of the Golgi apparatus within cells.
Product Datasheets
Citations for Human Phospho-eNOS (S1177) Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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Nitric Oxide Synthase Dependency in Hydroxyurea Inhibition of Erythroid Progenitor Growth
Authors: T Suboti?ki, OM Ajti?, D ?iki?, JF Santibanez, M Toši?, VP ?oki?
Genes, 2021-07-27;12(8):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Protective effect of eNOS overexpression against ischemia/reperfusion injury in small-for-size liver transplantation
Authors: Hai-Xin Qian
Exp Ther Med, 2016-09-30;12(5):3181-3188.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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Used 1/500 dilution for WB and obtained a very clean and clear individual band at the expected MW. Regular Bis Tris Criterion Gel electrophoresis from BioRad was run with MES buffer. Immobilion transfer membrane purchased from Millipore.
