Human Phospho-ErbB3/Her3 (Y1262) Antibody

Catalog #: AF5817 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5817
AF5817-SP

Detection of Human Phospho-ErbB3/Her3 (Y1262) by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Phospho-ErbB3/Her3 (Y1262) Antibody Summary

Species Reactivity

Human

Specificity

Detects human ErbB3 when phosphorylated at Y1262 in Western blots.

Source

Polyclonal Rabbit IgG

Purification

Antigen Affinity-purified

Immunogen

Phosphopeptide containing human ErbB3 Y1262 site

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Immunogen affinity purified

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Phospho-ErbB3/Her3 (Y1262) by Western Blot. Western blot shows lysates of MDA-MB-453 human breast cancer cell line untreated (-) or treated (+) with 300 ng/mL Recombinant Human NRG1-beta /HRG1-beta (Catalog # 396-HB) for 5 minutes. PVDF membrane was probed with 1 µg/mL of Human Phospho-ErbB3/Her3 (Y1262) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5817), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-ErbB3/Her3 (Y1262) at approximately 185 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ErbB3/Her3

ErbB3 (also known as HER3) is a type I transmembrane growth factor receptor and member of the human epidermal growth factor receptor (HER) family. ErbB3 is unique among HER family members in that it contains a defective kinase domain, and is therefore active only as a partner in a heterodimer complex with other ErbBs. Despite ErbB3's lack of intrinsic kinase activity, these heterodimers can be quite potent: the ErbB2/ErbB3 complex is considered the most active ErbB signaling dimer for the activation of downstream pathways. In addition, monomeric ErbB3 serves as a low affinity receptor for the heregulins (HRG), while ErbB2/ErbB3 heterodimers form a high affinity receptor complex. Phosphorylation of ErbB3 at Y1262 following ligand binding generates a binding site for the Grb2 adaptor protein.

Long Name

Receptor Tyrosine Protein Kinase ErbB3

Entrez Gene IDs

2065 (Human); 13867 (Mouse)

Alternate Names

c-erbB3; EC 2.7.10; EC 2.7.10.1; ErbB3; ErbB-3; erbB3-S; HER3; HER3c-erbB-3; LCCS2; lethal congenital contracture syndrome 2; MDA-BF-1; MGC88033; p180-ErbB3; p45-sErbB3; p85-sErbB3; Proto-oncogene-like protein c-ErbB-3; receptor tyrosine-protein kinase erbB-3; Tyrosine kinase-type cell surface receptor HER3; v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian)