Human Phospho-FAK (Y925) Antibody

Catalog #: MAB44671 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB44671
MAB44671-SP

Detection of Human Phospho-FAK (Y925) by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Phospho-FAK (Y925) Antibody Summary

Species Reactivity

Human

Specificity

Detects human FAK in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG_2B Clone # 743234

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Phosphopeptide containing the human FAK Y925 site

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Phospho-FAK (Y925) by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells untreated (-) or treated (+) with 1 mM Pervanadate (PV) for 5 minutes. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Phospho-FAK (Y925) Monoclonal Antibody (Catalog # MAB44671) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Phospho-FAK (Y925) at approximately 125 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FAK

Focal adhesion kinase 1 (FAK) is a ubiquitously expressed nonreceptor protein tyrosine kinase that is concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. This cellular localization is directed by a "Focal Adhesion Targeting" (FAT) sequence, a 125 amino acid sequence at the C-terminus. FAK plays an important role in migration, cell spreading, differentiation, cytoskeleton protein phosphorylation, apoptosis and acceleration of the G1 to S phase transition of the cell cycle. Increased expression and/or activity of FAK in various tumors has been correlated with enhanced migration and invasiveness of human tumor cells in addition to promoting increased cell proliferation. Src-dependent phosphorylation of FAK at Tyr925 regulates the effect of FAK on cell migration and tumor angiogenesis. Human FAK contains one FERM domain (aa 35-355) and one kinase domain (aa 415-684). Alternate splicing generates additional isoforms with N-terminal and C-terminal truncations. Within aa 919-930 human, mouse, and rat FAK share 100% aa sequence identity.

Long Name

Focal adhesion kinase 1

Entrez Gene IDs

5747 (Human); 14083 (Mouse); 25614 (Rat)

Alternate Names

EC 2.7.10; FADK 1; FADK1; FADKFAK-related non-kinase polypeptide; FAK; FAK1FRNK; FAKEC 2.7.10.2; focal adhesion kinase 1; pp125FAK; Protein-tyrosine kinase 2; PTK2 protein tyrosine kinase 2; PTK2