Human Phospho-IKK alpha (S176/S180) Antibody

Catalog #: MAB3768 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3768
MAB3768-SP

Detection of Human Phospho-IKK alpha (S176/S180) by Western Blot.

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Human Phospho-IKK alpha (S176/S180) Antibody Summary

Species Reactivity

Human

Specificity

Detects human IKK alpha when phosphorylated at S176/S180.

Source

Monoclonal Rat IgG_2A Clone # 829177

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Phosphopeptide containing the human IKK alpha S176/S180
Accession # O15111

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Phospho-IKK alpha (S176/S180) by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 100 nM Calyculin A (Catalog # 1336) and 20 ng/mL Recombinant Human TNF-a (Catalog # 210-TA) for 10 minutes. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human Phospho-IKKa (S176/S180) Mono-clonal Antibody (Catalog # MAB3768) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Phospho-IKKa (S176/S180) at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IKK alpha

I kappa B kinase alpha (IKK alpha ) is also known as IKBKA, CHUK, and IKK1. The classical active IKK complex, composed of IKK alpha, IKK beta, and two forms of processed IKK gamma, phosphorylates and inactivates I kappa B, resulting in the release and nuclear translocation of active NF-kappa B. IKK alpha contains kinase, leucine zipper, and helix-loop-helix domains, and heterodimerizes with IKK beta. NF-kappa B-inducing kinase (NIK) phosphorylates and activates IKK alpha.

Long Name

IkB Kinase alpha

Entrez Gene IDs

1147 (Human); 12675 (Mouse); 309361 (Rat)

Alternate Names

CHUK; conserved helix-loop-helix ubiquitous kinaseIKBKA; EC 2.7.11; EC 2.7.11.10; I-kappa-B kinase 1; I-kappa-B kinase alpha; IkappaB kinase; IkBKA; IkBKalpha; IKK alpha; IKK1; IKK1I-kappa-B kinase-alpha; IKK-A; IKKAIkB kinase alpha subunit; IKK-alphainhibitor of nuclear factor kappa-B kinase subunit alpha; NFKBIKA; NFKBIKAIKK-a kinase; Nuclear factor NFkappaB inhibitor kinase alpha; Nuclear factor NF-kappa-B inhibitor kinase alpha; TCF16; TCF-16; Transcription factor 16