Human Phospho-MSK1(S376)/MSK2(S360) Antibody
Human Phospho-MSK1(S376)/MSK2(S360) Antibody Summary
Accession # O75582
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Phospho-MSK1 (S376)/MSK2 (S360) by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 200 nM PMA for 20 minutes and 100 ng/mL Recombinant Human EGF (Catalog # 236-EG) for 5 minutes. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human Phospho-MSK1/MSK2 (MSK1 S376, MSK2 S360) Monoclonal Antibody (Catalog # MAB1094) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-MSK1 (S376)/MSK2 (S360) at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Human MSK1/MSK2 by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 200 nm PMA for 20 minutes, loaded at 0.2 mg/mL. A specific band was detected for MSK1/MSK2 at approximately 98 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human Phospho-MSK1/MSK2 (MSK1 S376, MSK2 S360) Monoclonal Antibody (Catalog # MAB1094). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MSK1/MSK2
Mitogen- and Stress-activated protein Kinases 1 and 2 (MSK1/2) have been shown to play key roles in the transcriptional regulation of immediate early genes such as c-fos. MSK1, also known as Ribosomal Protein S6 Kinase 5 (RPS6KA5), and MSK2, also known as RSKB and RPS6KA4, belong to the AGC family of kinases. Both proteins have two kinase domains connected by a regulatory linker region, and are activated by the mitogen-activated protein kinases ERK1, ERK2, and p38. Nuclear MSK phosphorylates and activates a number of transcription factors, including ATF1 and CREB. The phosphorylation of MSK1 at Ser376 or the equivalent Ser360 in MSK2 is required for kinase activity. These sites are located in the AGC kinase domain and are autophosphorylated. Their phosphorylation is essential for the catalytic activity of the N-terminal kinase domain. The sequence surrounding MSK1(S376) and MSK2(S360) is 100% identical.
Product Datasheets
Citation for Human Phospho-MSK1(S376)/MSK2(S360) Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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A Signaling Crosstalk Links SNAIL to the 37/67 kDa Laminin-1 Receptor Ribosomal Protein SA and Regulates the Acquisition of a Cancer Stem Cell Molecular Signature in U87 Glioblastoma Neurospheres
Authors: L Gresseau, ME Roy, S Duhamel, B Annabi
Cancers, 2022-11-30;14(23):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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