Human Phospho-Nbs1 (S343) Antibody

Catalog #: AF4944 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4944
AF4944-SP

Detection of Human Phospho-Nbs1 (S343) by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Phospho-Nbs1 (S343) Antibody Summary

Species Reactivity

Human

Specificity

Detects human Nbs1 when phosphorylated at S343. Does not recognize Nbs1 when unphosphorylated at S343.

Source

Polyclonal Rabbit IgG

Purification

Antigen Affinity-purified

Immunogen

Phosphopeptide containing human Nbs1 S343 site

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS and Sodium Azide with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Phospho-Nbs1 (S343) by Western Blot. Western blot shows Nbs1 immunoprecipitated from lysates of HeLa human cervical epithelial carcinoma cell line using Human Nbs1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1573). HeLa cell line was mock treated or exposed to 100 J/m²UV-C for 3 hours. PVDF membrane was probed with 1 µg/mL Rabbit Anti-Human Phospho-Nbs1 (S343) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4944) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-Nbs1 (S343) was detected at approximately 95 kDa (as indicated). For additional reference the membrane was stripped and reprobed with 1 µg/mL Human/Mouse/Rat Nbs1 Monoclonal Antibody (lower panel, Catalog # MAB1573) This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Nbs1

The Nijmegen Breakage Syndrome 1 (Nbs1) protein is a member of the Mre11/Rad50/Nbs1 (MRN) protein complex that binds to DNA double-strand breaks in cells exposed to DNA damaging agents. In addition, the MRN complex colocalizes with replication forks during DNA replication. The MRN complex plays an important role in routine cell cycle progression and genotoxic stress responses by facilitating DNA repair. Nbs1 is phosphorylated at S343 by ATM in response to double-strand breaks and by ATR under replication stress. This phosphorylation triggers the inactivation of late origin firing, which is essential for mediating the intra-S-phase checkpoint. Mutation of the nbs1 gene and resultant loss of Nbs1 protein expression in humans results in the chromosomal instability disease, Nijmegen Breakage Syndrome.

Long Name

Nijmegen Breakage Syndrome 1

Entrez Gene IDs

4683 (Human); 27354 (Mouse); 85482 (Rat)

Alternate Names

ATV; AT-V1; AT-V2; Cell cycle regulatory protein p95; FLJ10155; MGC87362; NBN; NBS; Nbs1; NBS1P95; Nibrin; Nijmegen breakage syndrome 1 (nibrin); Nijmegen breakage syndrome protein 1; p95 protein of the MRE11/RAD50 complex; p95